Immobilization of Acidithiobacillus ferrooxidans by a PVA–boric acid method for ferrous sulphate oxidation

Long, Zhong-er; Huang, Yunhong; Cai, Zhaoling; Cong, Wei; Ouyang, Fan

doi:10.1016/j.procbio.2003.11.004

Cited by 68 publications

(37 citation statements)

References 16 publications

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“…Compared with other immobilization materials, polyvinyl alcohol (PVA)-alginate based matrices boast many good properties for parameters such as the immobilized enzyme activity, reusability and stability. It was presumed that PVA might contribute to improve the durability and strength of the beads, while sodium alginate might improve the surface properties of the beads, reducing the tendency to agglomeration (Long et al, 2004). PVA-alginate beads have been used as a carrier for immobilized cells and enzymes (Idris et al, 2008;Nunes et al, 2010;Qin et al, 2010;Takei et al, 2011).…”

Section: Brazilian Journal Of Chemical Engineeringmentioning

confidence: 99%

Immobilization of phospholipase a1 using a polyvinyl alcohol-alginate matrix and evaluation of the effects of immobilization

Zhan

Jiang

Pan

2013

Braz. J. Chem. Eng.

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-The paper presents the synthesis and performance of an immobilized phospholipase A1 with practical application for oil degumming. The polyvinyl alcohol (PVA) had a number of properties indicating this polymer as a good enzyme carrier. The combination with alginate made a macro-porous structure, evidenced by SEM analyses. When the process time in boric acid solution was 30 minutes, the results revealed that beads prepared with 10% (w/v) PVA and 2% (w/v) sodium alginate in 4% (w/v) boric acid and 2% (w/v) calcium chloride solution exhibited high immobilized enzyme activity, immobilization yield and stability. The pH and temperature optimum for the PVA-alginate immobilized phospholipase A1were 5.6 and 58 °C, respectively. The enzyme immobilized in the beads retained 50.37% of the initial activity in the eighth cycle. The enzyme biocatalyst immobilized in the beads retained 78.58% of the initial activity after storing 6 weeks at 4 °C.

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Section: Brazilian Journal Of Chemical Engineeringmentioning

confidence: 99%

Immobilization of phospholipase a1 using a polyvinyl alcohol-alginate matrix and evaluation of the effects of immobilization

Zhan

Jiang

Pan

2013

Braz. J. Chem. Eng.

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“…Conversely, synthetic polymers have strong mechanical strength but are toxic to microorganisms (Zhang et al 2007). Recently, a promising type of synthetic polymer, polyvinyl alcohol (PVA), which is cheap and nontoxic to microorganisms, has been used for cell immobilization (Hashimoto and Furukawa 1987;Amanda and Wisecarver 1992;Asano et al 1992;Vogelsang et al 1997;Chang and Tseng 1998;Long et al 2004). A simple and economical technique of cell immobilization with PVA is PVA-boric acid method.…”

Section: Introductionmentioning

confidence: 99%

Reject water treatment by improvement of whole cell anammox entrapment using polyvinyl alcohol/alginate gel

et al. 2011

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Reject water treatment performance was investigated by whole cell anammox sludge entrapped polyvinyl alcohol/sodium alginate gel in the stirred tank reactor (STR). The whole experiment was conducted through Phase 1 and Phase 2 in which synthetic wastewater and modified reject water were used as feeding medium, respectively. The anammox reactor demonstrated quick start-up after 22 days as well as stable and relatively high nitrogen removal rate of more than 8.0 kg-N m(-3) day(-1) during the two both phases even under moderately low temperature of 25 ± 0.5°C during the last 2 months of Phase 2. The matured brownish red PVA beads had good characteristics with buoyant density of 1.10 g cm(-3), settling velocity of 141 m h(-1) and diameter of 4 mm. The bacterial community was identified by 16S rDNA analysis revealing the concurrent existence of KSU-1 and new kind anammox bacterium Kumadai-I after changing influent from synthetic wastewater to reject water. It was speculated that Kumadai-I might play a role as "promotion" factor together with KSU-1 on high nitrogen removal rate. These results demonstrate the potential application of whole cell anammox entrapment by PVA/alginate gel for achieving stable and high-rate nitrogen removal from high ammonium with low C/N ratio contained wastewaters, such as reject water, digester liquor or landfill leachate.

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“…Whole cell entrapment in calcium alginate, K-carrageenan and gerlite as gel matrix have also been attempted. 15,16 Cell entrapment in PVA cryogel as plain carrier 2,3 and in combination with boric acid, 17 with sodium alginate 18 and also passive cell attachment on siliceous stone particles, 19 ceramic beads 20 have been reported as good packed-bed supports for Acidithiobacillus ferrooxidans immobilization. The effect of surface roughness of active carbon fibers on immobilization of Acidithiobacillus ferrooxidans in inverse fluidized bed biofilm reactor 21 and the influence of different process variables on bio-oxidation by Acidithiobacillus ferrooxidans supported on LDPE particles in packed bed 22 have been recently reported.…”

Section: Introductionmentioning

confidence: 99%

Ferrous iron oxidation by foam immobilized Acidithiobacillus ferrooxidans: Experiments and modeling

Jaisankar

Modak

2009

Biotechnology Progress

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Ferrous iron bio-oxidation by Acidithiobacillus ferrooxidans immobilized on polyurethane foam was investigated. Cells were immobilized on foams by placing them in a growth environment and fully bacterially activated polyurethane foams (BAPUFs) were prepared by serial subculturing in batches with partially bacterially activated foam (pBAPUFs). The dependence of foam density on cell immobilization process, the effect of pH and BAPUF loading on ferrous oxidation were studied to choose operating parameters for continuous operations. With an objective to have high cell densities both in foam and the liquid phase, pretreated foams of density 50 kg/m(3) as cell support and ferrous oxidation at pH 1.5 to moderate the ferric precipitation were preferred. A novel basket-type bioreactor for continuous ferrous iron oxidation, which features a multiple effect of stirred tank in combination with recirculation, was designed and operated. The results were compared with that of a free cell and a sheet-type foam immobilized reactors. A fivefold increase in ferric iron productivity at 33.02 g/h/L of free volume in foam was achieved using basket-type bioreactor when compared to a free cell continuous system. A mathematical model for ferrous iron oxidation by Acidithiobacillus ferrooxidans cells immobilized on polyurethane foam was developed with cell growth in foam accounted by an effectiveness factor. The basic parameters of simulation were estimated using the experimental data on free cell growth as well as from cell attachment to foam under nongrowing conditions. The model predicted the phase of both oxidation of ferrous in shake flasks by pBAPUFs as well as by fully activated BAPUFs for different cell loadings in foam. Model for stirred tank basket bioreactor predicted within 5% both transient and steady state of the experiments closely for the simulated dilution rates. Bio-oxidation at high Fe(2+) concentrations were simulated with experiments when substrate and product inhibition coefficients were factored into cell growth kinetics.

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Immobilization of Acidithiobacillus ferrooxidans by a PVA–boric acid method for ferrous sulphate oxidation

Cited by 68 publications

References 16 publications

Immobilization of phospholipase a1 using a polyvinyl alcohol-alginate matrix and evaluation of the effects of immobilization

Immobilization of phospholipase a1 using a polyvinyl alcohol-alginate matrix and evaluation of the effects of immobilization

Reject water treatment by improvement of whole cell anammox entrapment using polyvinyl alcohol/alginate gel

Ferrous iron oxidation by foam immobilized Acidithiobacillus ferrooxidans: Experiments and modeling

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