Immobilization of acetylcholineesterase–choline oxidase on a gold–platinum bimetallic nanoparticles modified glassy carbon electrode for the sensitive detection of organophosphate pesticides, carbamates and nerve agents

Modified Cholinesterase Technology in the Construction of Biosensors for Organophosphorus Nerve Agents and Pesticides Detection

“…The cross-linking of enzyme with glutaraldehyde on an electropolymeric polyethyleneimine film on the electrode surface was used for example for potentiometric measurement in the paper (Reybier et al, 2002). Polyethylenimine as a carrier for ChE was used also for a screen electrode in the paper (Montesinos et al, 2001), chitosan with MWNT (Kandimalla & Ju, 2006) or with a glassy carbon electrode ; the bienzymatic AChE-ChO system is immobilized through glutaraldehyde to a glass electrode modified by Au-Pt nanoparticles (Upadhyay et al, 2009) etc. The self-assembled monolayer exploits the placement of a thin layer directly onto the converter.…”

Organophosphorous Compounds-Toxicity and Detection Approach

“…Various sensor based on bienzymatic approach have been developed for the detection of OP compounds (Ciucu et al, 2003, Ferapontova et al, 2001,Lin et al, 2004, Ferapontova et al, 2001, Kok et al, 2002, Upadhyay et al 2009). The ferrophthalocyanine (FePC) modified carbon paste electrodes are used for the construction of bienzymatic amperometric biosensor which is operated at low potential (0.35 V) for the detection of OP pesticides (Ciucu et al 2003).…”

“…The application of bimetallic nanoparticles (Bi-MNPs) is also reported (Fig. 6) for the sensitive detection of OP pesticides and nerve agents (Upadhyay et al, 2009). A novel sensitive amperometric biosensor based on electrodeposition of gold-platinum bimetallic nanoparticles onto 3-aminopropyltriethoxy silane modified glassy carbon electrode for the detection of paraoxon ethyl, aldicarb, and sarin has been developed.…”

“…bromide dioxime (TMB-4) (Amine et al, 2006, & Upadhyay et al, 2009. It has been found that the enzyme activity retains nearly 60% of initial activity with TMB-4, where as in the case of 2-PAM, the enzyme activity retention dropped to less than 50% of initial activity.…”

A bimetallic nanocoral Au decorated with Pt nanoflowers (bio)sensor for H2O2 detection at low potential

“…Nanostructures based on transition metals such as Au and Pt, thanks to their capability to enhance the electrocatalytic activity towards H 2 O 2 oxidation, have been incorporated into electrode surfaces to make biosensors [26][27][28][29][30]. Different studies demonstrated that the combination of two metallic nanostructures generates a new structure with different chemical and physical properties that were not observed by using one single metallic nanocatalyst alone [31][32][33]. According to the literature, AuPt hybrid nanostructures have become more attractive thanks to the synergistic catalytic effect and an excellent resistance to poisoning [34][35][36][37][38][39].…”