Immobilization effect of Ruta graveolens L. on human sperm: A new hope for male contraception

Harat, Zhila Naghibi; Sadeghi, Mohammad Reza; Sadeghipour, Hamid Reza; Kamalinejad, Mohammad; Eshraghian, Mohammad Reza

doi:10.1016/j.jep.2007.09.004

Cited by 45 publications

(27 citation statements)

References 41 publications

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“…Other natural sources of spermicidal action have been reported, such as scorpion toxins and plant extracts [Harat et al 2008;Lopez-Gonzalez et al 2003]. All these studies suggest new lines of research to identify the active compound(s) that mediate the modulation of ionicconductances, and that could provide a resource for a new generation of contraceptives.…”

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confidence: 99%

Venom of the ChileanLatrodectus mactansAlters Bovine Spermatozoa Calcium and Function by Blocking the TEA-sensitive K⁺Current

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Martı́nez-Torres²,

Gutiérrez

et al. 2010

Systems Biology in Reproductive Medicine

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The morphology and size of spermatozoa make it difficult to study the functional properties of the plasma membrane, however, some studies have revealed the presence of a number of ion channels in this cell. We measured the calcium (Ca ++ ) influx induced by depolarization of the plasma membrane and by venom isolated from the Chilean black widow spider (Latrodectus mactans), and functional changes in the presence of either high potassium or total venom. Our results indicate that the venom increased the Ca ++ influx,with an EC50 of 6.1 mg/mL and triggering the acrosome reaction in 43.26% of the cells. The application of potassium (10 mM K + ) or total venom (10 mg/ mL) did not affect the morphology or DNA stability of the sperm. The effects induced by high K + and venom suggest that direct blocking of K + currents alters the passive properties of the plasma membrane, leading to the entry of Ca ++ . These results show the importance of functional changes induced by depolarizing the spermatozoa and by venom. This venom possesses one or more molecules that may be used as pharmacological tools for studies on spermatozoa and have potential applications in reproductive biotechnology.

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confidence: 99%

Venom of the ChileanLatrodectus mactansAlters Bovine Spermatozoa Calcium and Function by Blocking the TEA-sensitive K⁺Current

Navarrete

Martı́nez-Torres²,

Gutiérrez

et al. 2010

Systems Biology in Reproductive Medicine

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“…Aqueous extract of Ruta graveolans has immobilizing effect on human sperm at a concentration of 100mg/ml of lyophilized form in 1:1 ratio can immediately immobile sperm cell in semen sample as it posseses acridine, alkaloids, acoumarine, essential flavonoids and furoquinolines. 21,28 Figure 6: Alium cepa. Compound made up of Abhal (Juniperus communis), Heeng (Ferula asafetida), Ushq (Dorema ammonicum) each 2 gm in the form of tablet result in miscarriage 29 or local application of paste made by 5 gm powder of Abhal, Suddab and Natroon (Pinus Sylvester) before coitus on penis act as contraceptive.…”

Section: Suddab/garden Rue (Ruta Graveolens) [Figure 7]mentioning

confidence: 99%

Evidence based approach to unani contraceptives: a review

Khan¹,

Shameem²

2016

Int J Reprod Contracept Obstet Gynecol

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“…Due to its cultural and medicinal value, rue has been introduced in various countries of North, Central and South America, China, India, Middle East and South Africa (Miguel, 2003). R. graveolens has been known as "Sodaab" in Iran and distributed in northern parts of Iran especially in Gilan Province (Harat et al, 2008). R. graveolenis a small evergreen sub-shrub or semi woody perennial 0.6 to 0.9 m tall and almost as wide.…”

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confidence: 99%

In vitro Antimicrobial Activity of Crude Extracts of Some Medicinal Plants from Al-Baha Region in Saudi Arabia

Al-Sokari¹

2015

JFNS

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Abstract:Medicinal plants are the wealthy source of antibacterial agents and curatives. There is an urgent need for new antimicrobial agents, so the antimicrobial activity for Ruta graveolens L. and Ficus carica Linn. Crude extracts were evaluated for its inhibitory activity against some disease causing selected microorganisms. Root extract of Ruta graveolens L gave 8 and 7 mm inhibition zone against Escherichia coli 254607 and Pseudomonas aeruginosa 254992 respectively. Latex of Ficus carica Linn plants showed that it is a good inhibitor for Candida albicans 254625; whereas the rest of microorganisms (Escherichia coli 254607, Proteus mirabilis 257440, Microsporium canis, Staphylococcus aureus 254996, Klebsiella pneumoniae 254656 and Staphylococcus epidermidis 254997) gave almost a similar inhibition zone around 2-3 mm. The tested crude extract from Ruta graveolens L. and Ficus carica Linn. have proved to be promising treating agents against the tested pathogenic microbes, but it needs to be concentrated and furthermore evaluated.

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Immobilization effect of Ruta graveolens L. on human sperm: A new hope for male contraception

Cited by 45 publications

References 41 publications

Venom of the ChileanLatrodectus mactansAlters Bovine Spermatozoa Calcium and Function by Blocking the TEA-sensitive K⁺Current

Venom of the ChileanLatrodectus mactansAlters Bovine Spermatozoa Calcium and Function by Blocking the TEA-sensitive K⁺Current

Evidence based approach to unani contraceptives: a review

In vitro Antimicrobial Activity of Crude Extracts of Some Medicinal Plants from Al-Baha Region in Saudi Arabia

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Immobilization effect of Ruta graveolens L. on human sperm: A new hope for male contraception

Cited by 45 publications

References 41 publications

Venom of the ChileanLatrodectus mactansAlters Bovine Spermatozoa Calcium and Function by Blocking the TEA-sensitive K+Current

Venom of the ChileanLatrodectus mactansAlters Bovine Spermatozoa Calcium and Function by Blocking the TEA-sensitive K+Current

Evidence based approach to unani contraceptives: a review

In vitro Antimicrobial Activity of Crude Extracts of Some Medicinal Plants from Al-Baha Region in Saudi Arabia

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Product

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Venom of the ChileanLatrodectus mactansAlters Bovine Spermatozoa Calcium and Function by Blocking the TEA-sensitive K⁺Current

Venom of the ChileanLatrodectus mactansAlters Bovine Spermatozoa Calcium and Function by Blocking the TEA-sensitive K⁺Current