Immobilization and characterization of recombinant<i>Candida antarctica</i>lipase B on poly(glycidyl methacrylate-ter-divinyl benzene-ter-ethylene dimethacrylate) beads, “DILBEADS™TA”

Rajasekar, Vyasa Williams; Tambe, Anita; Datla, Anupama

doi:10.3109/10242422.2013.775254

Cited by 4 publications

(2 citation statements)

References 33 publications

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“…Divinylbenzene has been used as crosslinker of many other polymeric matrices, like different acrylates, giving supports with a lower hydrophobicity [15][16][17][18].…”

Section: Poly-styrene-divinylbenzene Matrices As Supports To Immobilimentioning

confidence: 99%

Immobilization of Proteins in Poly-Styrene-Divinylbenzene Matrices: Functional Properties and Applications

Rafael¹,

Hernández²,

Barbosa³

et al. 2015

COC

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Supports based on poly-styrene-divinylbenzene (PSD) are commercially available since a long time ago. However, they are not commonly used as enzyme immobilization matrices. The main reason for this lies in the negative effect of the very hydrophobic surface on enzyme stability that produces the instantaneous enzyme inactivation in many instances. However, they have recently regained some impact on enzyme immobilization. They are easy to modify, and have been prepared with different modifiers. We will pay special attention to the coating of these supports with ionic liquids, which permits to have the ionic liquid phase anchored to the solid and modulate the enzyme properties without risk of losing these expensive and potentially toxic compounds. Thus, this review will present the covalent or physical immobilization of enzymes on PSD supports, submitted to different modifications. Moreover, lipases immobilized via interfacial activation on some naked PSD supports have shown some unexpected improvement in their catalytic properties, with uses in reactions like hydrolysis, esterification or transesterification.

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“…Divinylbenzene has been used as crosslinker of many other polymeric matrices, like different acrylates, giving supports with a lower hydrophobicity [15][16][17][18].…”

Section: Poly-styrene-divinylbenzene Matrices As Supports To Immobilimentioning

confidence: 99%

Immobilization of Proteins in Poly-Styrene-Divinylbenzene Matrices: Functional Properties and Applications

Rafael¹,

Hernández²,

Barbosa³

et al. 2015

COC

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“…32 Lipase immobilization on a water-insoluble carrier is an effective method to improve the biocatalyst performance of the enzyme. Different techniques can be used in the lipase immobilization process, such as adsorption, 148 covalent bonding, 378 encapsulation, 104 and entrapment. 25 Among them, the adsorption operation is simple and low-cost.…”

Section: Introductionmentioning

confidence: 99%

Enzyme functionalized solution blown nonwovens

Fnu¹,

Salmon²

2022

Preprint

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ASADUZZAMAN, FNU. Enzyme Functionalized Solution Blown Nonwovens. (Under the direction of Dr. Sonja Salmon). This Ph. D. dissertation presents the innovation and development of a new category of functional materials that uses a solution blown spinning (SBS) process to produce novel, useful biocatalytic functionalized nonwovens. SBS is a rapid, mild nanofiber formation process that is not limited to thermal-plastic polymers or high dielectric constant solvent-soluble polymers. With the SBS technique, it was possible to achieve single-step enzyme immobilization into polymeric carriers from enzyme-compatible polymer solutions. The goals of this research were to explore techniques for preparing polymer-solvent-enzyme compatible triads, optimize solution spinning processes for producing enzyme functionalized solution blown nonwovens (EFSBN), and validate the anticipated application features of EFSBN.Chapter 1 is a review of the literature to assess the potential for compatible polymer-solventenzyme triads for co-immobilization solution processing to increase immobilization yield and reduce immobilization complexity. The particular focus is to produce enzyme compatible polymer solutions in an organic solvent for solution blown spinning process where in-situ fiber formation and enzyme immobilization happen. In-situ co-immobilization is one of the promising methods in enzyme immobilization due to its high enzyme loading, versatility, and single-step fabrication processability. However, the stability and compatibility of enzymes in polymer solutions (especially organic solvent-soluble polymer solutions) are challenging. Enzymes may denature in the presence of organic solvents or at elevated temperatures, limiting how enzymes can be combined with organic solvent-soluble polymers. This chapter discusses strategies for producing compatible polymer-solvent-enzyme triads that span aqueous and non-aqueous fabrication requirements.Chapter 2 reports the novel production of unique enzyme functionalized solution blown nonwoven (EFBSN) webs from aqueous soluble polyethylene oxide (PEO) polymer solution by the solution blow spinning method. Protease co-immobilization via entrapment in PEO nanofibers by solution blowing was demonstrated as a simple and efficient process for loading a broad concentration range of enzymes. The rapidly water-soluble and non-dusting EFSBN solid materials preserve a high enzyme activity level over long periods of ambient storage without adding a stabilizer and are easy to handle, making EFSBNs a potential alternative format for delivering enzymes in products that require fast-dissolving solid formulations, like detergents.Chapter 3 reveals an approach to produce an enzyme (CALB) compatible polycaprolactone (PCL)chloroform solution for enzyme co-immobilization via entrapment in PCL nanofibers. CALB enzymes survived in the microemulsion (water-in-oil) of the PCL-chloroform-CALB compatible triad and retained around half of their initial activity after solution blow spinning. These novel CALB-loaded-EFSBN-PCL ...

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Ultrasound–assisted enzymatic biodiesel production using blended feedstock of non–edible oils: Kinetic analysis

Malani

Umriwad

Kumar

et al. 2019

Energy Conversion and Management

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Immobilization and characterization of recombinantCandida antarcticalipase B on poly(glycidyl methacrylate-ter-divinyl benzene-ter-ethylene dimethacrylate) beads, “DILBEADS™TA”

Cited by 4 publications

References 33 publications

Immobilization of Proteins in Poly-Styrene-Divinylbenzene Matrices: Functional Properties and Applications

Immobilization of Proteins in Poly-Styrene-Divinylbenzene Matrices: Functional Properties and Applications

Enzyme functionalized solution blown nonwovens

Ultrasound–assisted enzymatic biodiesel production using blended feedstock of non–edible oils: Kinetic analysis

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