Immobilisation of engineered molecules on electrodes and optical surfaces

Reversible immobilization of engineered molecules by Ni-NTA chelators

“…An impermeable dye layer, screen-printed on the copper surface, was used as a mask and FeCl 3 dissolved the exposed Cu, leaving the desired electrode geometries under the dye (later easily removed with acetone). Chemical Au plating on Cu was obtained with Au(CN) 2 À and Au(CN) 4 À . An Au wire was dissolved in boiling HCl/HNO 3 (3:1) and then dried at 70-80 jC; HCl 37% was added and the solution dried again; finally, HCl 0.1 M was added to have AuCl 3 (0.6 g/l) in solution which was mixed with KCN (10.0 g/l), Na 2 HPO 4 (6.0 g/l), NaOH (1.0 g/l), Na 2 SO 3 (3.0 g/l).…”

“…An original procedure, suitable for chemical immobilization of engineered (His) 6 -tagged proteins on Au or graphite surfaces, was previously reported [2] with the result of obtaining oriented and highly specific immobilization of two engineered proteins [1]. Renewable specific binding of (His) 6 -proteins to sensor surfaces and fast and sensitive electrochemical or optical detection of analytes were also obtained [2]. ''On chip'' protein pre-concentration was conveniently achieved for biosensing purposes, starting from crude unpurified extracts and avoiding protein purification steps [2].…”

“…Renewable specific binding of (His) 6 -proteins to sensor surfaces and fast and sensitive electrochemical or optical detection of analytes were also obtained [2]. ''On chip'' protein pre-concentration was conveniently achieved for biosensing purposes, starting from crude unpurified extracts and avoiding protein purification steps [2]. In this paper, selective electrochemical synthesis of CYS layers on Au, Pt and graphite was studied and compared, using a model molecule (Photosystem II, PSII) with the aim of biosensors improvement.…”

Biosensors based on gold nanoelectrode ensembles and screen printed electrodes

“…The PANI/NEE/SPS was immersed in a solution of 10% v/v APTES for 90 min, in order to obtain a terminal amino group (-NH 2 ) onto the PC surface [35,37]. The resulting derivative was accurately washed with PB solution (pH 6.8) and water.…”

Development and Characterization of Nickel‐NTA‐Polyaniline Modified Electrodes

“…The His-tag has enough flexibility to bind to both unoccupied sites of the Me 2þ -NTA complex synergistically. -NTA has been used as the basis of protein deposition at gold and graphite surfaces using self-assembled monolayers for applications in both optical and electrochemical immunosensors [10,11]. Some researchers have tried to directly exploit the targeted interaction of amino acids with conducting polymers such as polyaniline.…”