Development and Characterization of Nickel‐NTA‐Polyaniline Modified Electrodes

Abstract

The engineered addition of hexa-histidine sequences to biomolecules such as antibody fragments has been found to be an excellent means of purifying these materials. This tagging methodology has also been extended to its use as a tool for immobilization and orientation of antibodies on transducer surfaces. Polyvinyl sulfonate-doped polyanilne (PANI/ PVS) can be used as a mediator in amperometric biosensors. This short communication looks at the effect of nickel chelate materials and nickel chelation on this conducting polymer and evaluates it as a potential surface for the immobilization of his-tagged biomolecules. N-nitrilotriacetic acid (NTA) was doped into the electropolymerized PANI/PVS at a screen-printed carbon paste electrode. The resulting NTA-PANI/PVS film was shown to have comparable electrochemical properties of polymer without the chelating agent. When Ni 2þ was applied to the electrode, the incorporated NTA was found to efficiently chelate the metal ions at the electrode surface.

Electrochemical detection of copper ion using a modified copolythiophene electrode

“…The IDA group forms a tridendate tetrahedral complex with Cu 2+ [15,20,21], which has enabled wide applications of the materials containing IDA groups for separation of copper ions from complex media [16].…”

Electrochemical Sensors Based on Organic Conjugated Polymers

“…Single stranded DNA has been immobilized on self-assembling polymer based on polyallyamine modified with thioctic acid for hybridization assays [ 134 ]. Lori et al has developed a conducting polymer surface by doping N -nitriloacetic acid (NTA) into the electropolymerized polyvinylsulphonate doped polyaniline (PANI/PVS) at a screen printed carbon electrode for the immobilization of his -tagged biomolecules [ 135 ]. The resulting NTA-PANI-/PVS film was shown to have interesting electrochemical properties.…”

Recent Advances in Biological Sensors Based on Electrogenerated Polymers: A Review

“…In addition, the catalytic site of the enzyme can also be blocked by these uncontrolled immobilization methods. More recently, NTA was efficiently doped into an electropolymerized polyanilinepoly(vinyl sulphonate) film without alteration of its electronic properties of conducting polymer (Lori et al 2006). In addition, the resulting polymer was able to coordinate Ni(II) and hence may be an attractive platform for the future development of immunosensors based on an impedimetric or conductimetric transduction.…”