Electrochemical detection of copper ion using a modified copolythiophene electrode

Lin, Meng; Cho, MiSuk; Choe, Wonyoung; Son, Younggon; Lee, Youngkwan

doi:10.1016/j.electacta.2009.07.025

Cited by 92 publications

(35 citation statements)

References 29 publications

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“…In life sciences it is a challenge the visualization of strictly regulated availability of copper in the tight copper-binding environment of the cell. The selective recognition of these ions is also a great challenge because they have similar magnitude, valence and coordinating properties with some other metals [5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Fluorescence of a Histidine-Modified Enhanced Green Fluorescent Protein (EGFP) Effectively Quenched by Copper(II) Ions. Part II. Molecular Determinants

et al. 2015

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The histidine-modified EGFP was characterized as a sensing element that preferentially binds nanomolar concentrations of Cu(2+) in a reversible manner (Kd = 15 nM). This research aims to determine the causes of nanomolar-affinity of this mutant by investigating significant structural and energetic alterations of the chromophore in the presence of different copper ion concentrations. In order to reveal the unknown parts of the quenching mechanism we have elaborated a specific approach that combines theoretical and experimental techniques. The theoretical experiment included the modeling of potential distortions of the chromophores and the corresponding changes in energy using quantum mechanical calculations. Differences between the modeled energy profiles of planar and distorted conformations represented the energies of activation for the chromophore distortions. We found that some values of the experimental activation energies, which were derived from fluorescence lifetime decay analysis (ex: 470 nm, em: 507 nm), were consistent with the theoretical ones. Thus, it has been revealed similarity between the theoretical activation energy (50 kJmol(-1)) for 40° phenolate-ring distortion and the experimental activation energy (52.17 kJmol(-1)) required for histidine-modified EGFP saturation with copper. This chromophore conformation was further investigated and it has been found that the large decrease in fluorescence emission is attributed to the significant charge transfer over the molecule which triggers proton transfer thereby neutralizing the cromophore.

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Section: Introductionmentioning

confidence: 99%

Fluorescence of a Histidine-Modified Enhanced Green Fluorescent Protein (EGFP) Effectively Quenched by Copper(II) Ions. Part II. Molecular Determinants

et al. 2015

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“…15 CPs have been widely investigated for applications in chemical sensors and biosensors. 16,17 Among the various CPs, polypyrrole (PPy) plays an important role, due to its high electrochemical activity and conductivity, and biocompatibility. 18 PPy is technologically important, notably due to its environmental stability and high electronic conductivity, especially for fibers having a diameter of less than 150 nm.…”

Section: Introductionmentioning

confidence: 99%

Hydrogen peroxide detection using a polypyrrole/Prussian blue nanowire modified electrode

et al. 2011

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A hydrogen peroxide sensor based on a polypyrrole (PPy)/Prussian blue (PB) modified electrode was developed. PPy nanowires were prepared by one step template-free electrochemical polymerization on a gold electrode and a PB layer was deposited using an electrochemical method. The introduction of the PPy nanostructure can provide a high surface area and lead to high sensitivity to hydrogen peroxide. The surface morphology of the PPy/PB electrode was characterized by scanning electron microscopy (SEM). The structural features of the PPy/ PB electrode were confirmed by attenuated total reflection infrared (ATR-IR) and X-ray photoelectron spectroscopy (XPS). Chronoamperometry (CA) was used to evaluate the sensitivity of the sensor towards hydrogen peroxide.

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“…4a), the peak current of the PPy-COOH NT arrays decreases distinctly after incorporation of Py-␣-COOH onto the surface of PPy NT arrays [36]. nanoarrays shows no obvious electrochemical response between −0.6 and +0.6 V. For the Cu 2+ captured electrode, a few apparent redox peaks appeared at −0.148 V and −0.168 V. These peaks are clearly attributed to the redox reaction of Cu 0 /Cu 2+ [26,27], confirming the attachment of GGH tripeptide to the PPy-COOH NT arrays electrode.…”

Section: Cyclic Voltammetrymentioning

confidence: 99%

“…The modification of PPy-COOH NT array electrode with tripeptide (GGH) was performed as reported elsewhere [26,27]. The carboxyl terminal groups of the PPy-COOH were activated in a solution of 50 mM EDC/50 mM NHS in 100 mM MES (pH 6.8) for 3 h. After a thorough rinse with 25 mM MES buffer, the polymer electrode was reacted overnight with GGH (3 mg mL −1 ) in 100 mM MES buffer to form the peptide conjugated PPy-COOH (PPy-GGH) electrode.…”

Section: Modification Of Ppy-cooh Nt Array Electrode With Ggh Tripeptidementioning

confidence: 99%

Functionalized polypyrrole nanotube arrays as electrochemical biosensor for the determination of copper ions

Lin

Ma³

et al. 2012

Analytica Chimica Acta

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Electrochemical detection of copper ion using a modified copolythiophene electrode

Cited by 92 publications

References 29 publications

Fluorescence of a Histidine-Modified Enhanced Green Fluorescent Protein (EGFP) Effectively Quenched by Copper(II) Ions. Part II. Molecular Determinants

Fluorescence of a Histidine-Modified Enhanced Green Fluorescent Protein (EGFP) Effectively Quenched by Copper(II) Ions. Part II. Molecular Determinants

Hydrogen peroxide detection using a polypyrrole/Prussian blue nanowire modified electrode

Functionalized polypyrrole nanotube arrays as electrochemical biosensor for the determination of copper ions

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