As
one of the eight essential amino acids in the human body, l-isoleucine plays an important role in human life metabolism
and can be applied as a nutritional supplement and food additive in
the food field. However, the crystallization of l-isoleucine
is generally flaky with poor powder properties, such as bulk density
and flowability, which is not conducive to a wide range of industrial
applications. In this study, we successfully prepared large spherical
particle crystals via two different ways of crystallization. l-Isoleucine undergoes the evolution from initial round flakes to
three-dimensional petal-like structures and finally to spherical particles
by evaporation crystallization in the presence of hydroxypropylmethylcellulose
(HPMC). l-Isoleucine also aggregates into spherical particles
in the acid–base reaction crystallization process by adding
a small amount of suitable and volatile bridging liquids, such as
cyclohexane and ethyl acetate. In conclusion, both approaches improved
the crystal properties of l-isoleucine and produced spherical
particles with a larger particle size, higher bulk density, and higher
compression resistance, thereby providing new insight into the preparation
of high-quality l-isoleucine.