Imaging of ovarian cancers using enzyme activatable probes with second near-infrared window emission

Abstract

A β-galactosidase (β-Gal) activatable NIR-II fluorescent probe for visualizing ovarian cancers.

Enzyme-activatable fluorescent probes for β-galactosidase: from design to biological applications

“…7b), which were also incorporated with a Dgalactose residue as a b-gal activatable trigger. 71 Upon titration with b-gal, BOD-K-bgal and BOD-M-bgal displayed signicant turn-on NIR-I or NIR-II responses, respectively. Furthermore, the in vivo ovarian tumor imaging was carried out in the SKOV3 subcutaneous xenogra nude mice.…”

Activatable NIR‐II Fluorescent Probes Applied in Biomedicine: Progress and Perspectives

“…Therefore, detecting the concentration of these molecules in vivo has important research significance for prevention and detection of related diseases. In this part, we emphatically describe the representative development of reactive oxygen species (ROS), [70–73] gas, [74,75] enzymes [76–78] and pH sensing [79–81] based on NIR‐II AFPs. In order to broaden the application of NIR‐II AFPs, designs and applications of them are detailed introduction in the following, and representative of NIR‐II AFPs were summarized in Table 2.…”

Molecular Engineering of NIR‐II Fluorophores for Improved Biomedical Detection

“…Therefore,t his BODIPYbased platform can conveniently serve as an off-on NIR-II fluorescent probe upon reaction with H 2 S, [57] b-galactosidase (b-gal), nitroreductase (NRT), alkaline phosphatase (ALP), or NAD(P)H:quinone oxidoreductase isozyme (NQO), or as av iscosity probe (Figure 5B). [58] Beside substituent modification, the general fluorescence quenching associated with the nitro group can also be applied in NIR-II probes.F or example,t he fluorescence of IR-1048 is quenched by the nitro-imidazole unit and can recover after reduction of the nitro group (53 to 54,Figure 5C). [59] Most fluorophores suffer from aggregation-caused quenching (ACQ).…”