Imaging of innate immunity activation in vivo with a redox-tuned PET reporter

Pisaneschi, Federica; Gammon, Seth T.; Paolillo, Vincenzo; Qureshy, Sarah; Piwnica‐Worms, David

doi:10.1038/s41587-021-01169-y

Cited by 13 publications

(10 citation statements)

References 58 publications

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“…All these data reinforced a promising approach relying on a combination of immune therapy and carbon therapy to target radioresistant tumours. More recently, ROS and RNS were shown as key effectors of innate immunity and activators of some immune pathways [98].…”

Section: Immune Responsementioning

confidence: 99%

The ‘stealth-bomber’ paradigm for deciphering the tumour response to carbon-ion irradiation

Wozny

Rodriguez-Lafrasse

2023

Br J Cancer

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Numerous studies have demonstrated the higher biological efficacy of carbon-ion irradiation (C-ions) and their ballistic precision compared with photons. At the nanometre scale, the reactive oxygen species (ROS) produced by radiation and responsible for the indirect effects are differentially distributed according to the type of radiation. Photon irradiation induces a homogeneous ROS distribution, whereas ROS remain condensed in clusters in the C-ions tracks. Based on this linear energy transfer-dependent differential nanometric ROS distribution, we propose that the higher biological efficacy and specificities of the molecular response to C-ions rely on a ‘stealth-bomber’ effect. When biological targets are on the trajectories of the particles, the clustered radicals in the tracks are responsible for a ‘bomber’ effect. Furthermore, the low proportion of ROS outside the tracks is not able to trigger the cellular mechanisms of defence and proliferation. The ability of C-ions to deceive the cellular defence of the cancer cells is then categorised as a ‘stealth’ effect. This review aims to classify the biological arguments supporting the paradigm of the ‘stealth-bomber’ as responsible for the biological superiority of C-ions compared with photons. It also explains how and why C-ions will always be more efficient for treating patients with radioresistant cancers than conventional radiotherapy.

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Section: Immune Responsementioning

confidence: 99%

The ‘stealth-bomber’ paradigm for deciphering the tumour response to carbon-ion irradiation

Wozny

Rodriguez-Lafrasse

2023

Br J Cancer

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“…Platelet endothelial cell adhesion molecule (CD31) acts as a central regulator of inflammatory responses, modulating the recruitment and extravasation of immune cells at sites of plateletcoated and inflamed microvessels. 𝛼-SMA is widely expressed in tissues with severe fibrosis, while myeloperoxidase (MPO) is part of an organism's defense system, [53] reflecting the severity of the inflammatory response in tissues with pathogen infection. [54,55] The expression levels of CD31 (in pink), 𝛼-SMA (in green), and MPO (in pink) in the kidney and large intestine were investigated through immunofluorescent staining.…”

Section: Photodynamic Blood Disinfection For Sepsis Treatment In Vivomentioning

confidence: 99%

Aggregation‐Induced Emission Photosensitizer‐Armored Magnetic Nanoparticles for Sepsis Treatment: Combating Multidrug‐Resistant Bacteria and Alleviating Inflammation

Wang,

Chen,

Song

et al. 2023

Adv Funct Materials

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Sepsis, a life‐threatening condition stemming from an uncontrolled host immune response to bacterial infections, continues to impose a significant global burden with high morbidity and mortality. Addressing the challenges posed by antimicrobial resistance and uncontrollable inflammation remains a challenge in sepsis treatment. Moreover, traditional antibacterial materials have low bacterial trapping efficiency and inevitable prolonged circulation within the bloodstream, resulting in suboptimal antibacterial effects, metabolic complications, and undesirable side effects. In this study, an innovative solution is introduced through the development of Fe3O4@SH@TBTCP‐PMB, an aggregation‐induced emission (AIE) photosensitizer (PS)‐armored magnetic nanoparticles (NPs). It has high reactive oxygen species (ROS) generation efficiency and an exceptional ability to capture Gram‐positive bacteria with over 80% enrichment efficiency within just 1 h, even at low bacterial concentrations. Under white light illumination, 100 µg mL−1 of Fe3O4@SH@TBTCP‐PMB effectively eliminated more than 99.9% of methicillin‐resistant Staphylococcus aureus (MRSA). Furthermore, its magnetic separation properties efficiently prevent systemic blood circulation and associated side effects. Most importantly, Fe3O4@SH@TBTCP‐PMB demonstrates superior anti‐inflammatory effects by regulating cytokines, reducing adhesion molecule expression, and managing oxidative stress levels. This multifunctional approach significantly enhances sepsis survival rates, offering a promising strategy for combating multidrug‐resistant (MDR) bacterial infections in sepsis patients while addressing inflammation‐related complications.

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“…Piwnica-worms et al identified a new molecule 4-[ 18 F]fluoro-1-naphthol ([ 18 F]4FN) that could selectively react with high-energy free radicals and be used for whole-body imaging by PET/CT to detect multiorgan inflammation in real time. 119 When oxidized by myeloperoxidase plus H 2 O 2 , [ 18 F]4FN selectively binds to nearby proteins and cells. Studies have shown that [ 18 F]4FN is superior to [ 18 F]FDG in detecting inflammation.…”

Section: Immune-related Imaging Strategiesmentioning

confidence: 99%