1994
DOI: 10.1002/sca.4950160409
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Imaging of immunolabeled membrane receptors in uncoated sem specimens

Abstract: Summary: Epidermal growth factor receptors (EGFR) were labeled with I0 n m immunogold and examined on uncoated specimens of A43 I human epidermoid carcinomacells. A field emission gun and a high-sensitivity YAG ring detector were used to demonstrate the affinity labeling simultaneously in the secondary-electron (SE) and backscattered-electron (BSE) modes with a low accelerating voltage (V,). At V0=2 kV, the SE and BSE signals were too weak to identify all markers, while at Vc,=3-7 kV labeling was observed unam… Show more

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