High‐resolution scanning electron microscopy of immunogold‐labelled cells by the use of thin plasma coating of osmium

Suzuki, Emiko

doi:10.1046/j.1365-2818.2002.01082.x

Cited by 108 publications

(58 citation statements)

References 19 publications

(18 reference statements)

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“…Since the shell material is a non-conductive material, the samples were initially coated with 5 nm thick gold layer in order to increase their electrical conductivity before the microscopy analysis was carried out. This procedure was in accordance with a similar study carried out by Suzuki [22].…”

Section: Scanning Electron Microscopy (Sem) Analysissupporting

confidence: 61%

Development of microencapsulated phase change material for solar thermal energy storage

Darkwa

Kokogiannakis

2017

Applied Thermal Engineering

119

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In this paper a novel microencapsulated phase change material (MF-3) has been developed and tested for solar assisted hot water storage systems. Even though the morphology of the sample was affected by the type of emulsifier used for fabrication it recorded the highest energy storage capacity of 126 kJ/kg with encapsulation efficiency of 97.4% as compared with other developed samples. For the purpose of assessing its thermal effectiveness it was theoretically evaluated in a compacted fixed bed TES unit and found to be capable of achieving a higher energy storage density as well as relatively smaller physical storage size than water based system. Despite the overall effective thermal conductivity being slightly less than water, its value was still about twice as high as most current PCM storage units. Experimental evaluation is therefore strongly encouraged.

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Section: Scanning Electron Microscopy (Sem) Analysissupporting

confidence: 61%

Development of microencapsulated phase change material for solar thermal energy storage

Darkwa

Kokogiannakis

2017

Applied Thermal Engineering

119

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“…This can provide a significantly thin conductive coating of down to < 1 nm (although usually, a few to several nm thick) composed of extremely fine amorphous particles that are virtually invisible and do not obscure the fine surface structure of specimens at very high-magnification observation. Therefore, thin osmium coatings have been used preferentially for the SEM imaging of biological textures such as found in cells, tissues, and membranes (e.g., Osawa and Nozaka, 1998;Akahori et al, 2000;Suzuki, 2002). The extreme thinness of the osmium coating may also be favorable for chemical quantitative analysis via electron microprobe, although the Z number of osmium is as large as those of gold and platinum (i.e., high X-ray attenuation).…”

Section: Introductionmentioning

confidence: 99%

EDS quantification of light elements using osmium surface coating

Ohfuji

Yamamoto

2015

Journal of Mineralogical and Petrological Sciences

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This study demonstrates the validity of a thin osmium coating for quantitative energy-dispersive spectroscopic (EDS) analysis, particularly for light elements such as O (and potentially C and N) in natural/synthetic minerals. An osmium coating prepared by chemical vapor deposition provides an extremely thin and uniform layer whose thickness can be controlled simply by coating time. Because of the high reproducibility and reliability of the osmium coating process, users have no difficulty in evaluating the actual coating thickness, which enables strict and precise absorption corrections (for the coating layer), even for low-energy characteristic X-rays, which are susceptible to attenuation by the coating layer itself. Our results show that oxygen concentrations in silicate and oxide minerals can be quantified correctly when using the osmium coating, whereas quantification using a carbon coating afforded values that were a few wt% lower than stoichiometry, probably due to the uncertainty of the actual coating thickness (i.e., the absorption correction was incorrect). The ability to accurately quantify oxygen may stimulate new analytical applications, such as the estimation of Fe 2+ /Fe 3+ concentrations and water content in minerals. Furthermore, the Os-coated samples prepared for EDS analysis are also suitable for electron back-scattered diffraction (EBSD) analysis without re-polishing and re-coating, which are usually routine but time-consuming tasks in the case of carbon-coated samples.

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“…When the SEM is used for biological materials, the specimens need to be killed, preserved, and stabilized (1). These complex procedures preclude the observation of living organisms and often produce unwanted artifacts.…”

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confidence: 99%

A thin polymer membrane, nano-suit, enhancing survival across the continuum between air and high vacuum

Takaku

Suzuki²,

Ohta

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

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Most multicellular organisms can only survive under atmospheric pressure. The reduced pressure of a high vacuum usually leads to rapid dehydration and death. Here we show that a simple surface modification can render multicellular organisms strongly tolerant to high vacuum. Animals that collapsed under high vacuum continued to move following exposure of their natural extracellular surface layer (or that of an artificial coat-like polysorbitan monolaurate) to an electron beam or plasma ionization (i.e., conditions known to enhance polymer formation). Transmission electron microscopic observations revealed the existence of a thin polymerized extra layer on the surface of the animal. The layer acts as a flexible "nano-suit" barrier to the passage of gases and liquids and thus protects the organism. Furthermore, the biocompatible molecule, the component of the nano-suit, was fabricated into a "biomimetic" free-standing membrane. This concept will allow biology-related fields especially to use these membranes for several applications.animal behavior | biophysics | microscopy | nanotechnology | plasma physics

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High‐resolution scanning electron microscopy of immunogold‐labelled cells by the use of thin plasma coating of osmium

Cited by 108 publications

References 19 publications

Development of microencapsulated phase change material for solar thermal energy storage

Development of microencapsulated phase change material for solar thermal energy storage

EDS quantification of light elements using osmium surface coating

A thin polymer membrane, nano-suit, enhancing survival across the continuum between air and high vacuum

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