Imaging of a Large Collection of Human Embryo Using a Super-Parallel MR Microscope

Matsuda, Yoshimasa; Ono, Shimpei; Otake, Yosuke; Handa, Shinya; Kose, Katsumi; Haishi, Tomoyuki; Yamada, Shigeto; Chikako, Uwabe; Shiota, Kohei

doi:10.2463/mrms.6.139

Cited by 46 publications

(42 citation statements)

References 7 publications

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“…Approximately 1,200 well-preserved human embryos diagnosed as externally normal at CS13 to CS23 were selected for MR microscopic imaging. The conditions used to acquire the MR images of the embryos are described elsewhere Matsuda et al, 2003Matsuda et al, , 2007Yamada et al, 2010).…”

Section: Materials and Methods Human Embryo Specimensmentioning

confidence: 99%

“…MR imaging of embryos is highly advantageous (Effmann et al, 1988;Smith et al, 1992;Haishi et al, 2001), providing a resolution of 40 lm/pixel or better with long scan times. Kyoto and Tsukuba Universities began a project in 1999 to acquire 3D MR microscopic images of thousands of human embryos using a super-parallel MR microscope operated at 2.34T Matsuda et al, 2003Matsuda et al, , 2007Yamada et al, 2006).…”

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confidence: 99%

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Embryonic Liver Morphology and Morphometry by Magnetic Resonance Microscopic Imaging

Hirose

Nakashima

Yamada

et al. 2011

The Anatomical Record

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Embryonic liver has a unique external morphology and quantitative morphometry, based on magnetic resonance imaging data of human embryos from the Kyoto Collection of Human Embryos. Liver morphogenesis is strongly affected by the adjacent organs and tissues. The left ventricle develops to the left medial-caudal side, which results in the formation of a depression at left medial region and a prominence bilaterally at the cranial surface of the liver between Carnegie Stage (CS)17 and CS19. An imprint of the stomach that formed at the dorsal left-medial region of the liver became more marked with development until CS23. A depression induced by the umbilicus formed at the ventral region of the liver between CS16 and CS19. An indentation caused by the right adrenal gland formed at the dorsal-caudal region of the liver surface from CS20. Morphometric analysis revealed that the volume of the liver increased exponentially from CS14 through CS23. The liver developed preferentially along the dorsoventral axis and right/left axis until CS17, along the craniocaudal axis between CS17 and CS19, and then in all directions after CS19. Several important developmental phenomena, such as differentiation of the diaphragm, the extension of the body axis of the embryo, and the physiologic herniation of the intestine into the umbilical cord, may affect morphometric data. These data contribute to a better understanding of liver development as well as the morphogenesis of adjacent organs, both temporally and spatially, and serve as a useful reference for fetal medicine and prenatal diagnosis. Anat Rec, 295:51-59, 2012. V V C 2011 Wiley Periodicals, Inc.

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Section: Materials and Methods Human Embryo Specimensmentioning

confidence: 99%

mentioning

confidence: 99%

Embryonic Liver Morphology and Morphometry by Magnetic Resonance Microscopic Imaging

Hirose

Nakashima

Yamada

et al. 2011

The Anatomical Record

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“…For example, Dhenain et al 4 used MRI to produce a digital mouse atlas, while Matsuda et al 2 produced a 3D image database for human embryology. 2.…”

Section: Described Reconstructed Human Bone By Micro-ctmentioning

confidence: 99%

“…Moreover, related technologies have been developed to meet changing medical demands. [1][2][3][4] Micro-CT is one such technology that is applied in the laboratory owing to its relatively high resolution and smaller apparatus. Roughly two decades have passed since the fi rst use of micro-CT, when it was applied to trabecular bone analysis.…”

Section: Introductionmentioning

confidence: 99%

Three-dimensional observation of the mouse embryo by micro-computed tomography: composition of the trigeminal ganglion

2010

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The purpose of this study was to demonstrate a micro-computed tomography (CT) method for observations of the mouse embryo. At 13.0 days post-coitum, mouse embryos were fixed in 4% paraformaldehyde for 24 h and stained en bloc by osmium tetroxide overnight. The embryos were then embedded in paraffin using standard methods for 24 h. Specimens were analyzed by micro-CT and image processing was performed. Organs containing nervous and blood systems could be viewed as a result of different osmium-staining densities. The trigeminal ganglion was imaged using three-dimensional techniques. Observation of the embryo was possible by micro-CT with osmium tetroxide staining.

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“…The Kyoto Human Embryo MR Microscopy Project was initiated to observe internal structures in a noninvasive manner in May of 2003. Consequently, 3-D MR microscopic images of 1,204 embryos were acquired at the spatial resolution of 40-150 μm 3 using a 2.34-T MRM (super-parallel MRM) at the Institute of Applied Physics, University of Tsukuba [Matsuda et al, 2007] (Fig. 2b).…”

Section: Mr Microscopymentioning

confidence: 99%

The Kyoto Collection of Human Embryos and Fetuses: History and Recent Advancements in Modern Methods

Yamaguchi

Yamada

2018

Cells Tissues Organs

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The Kyoto Collection of Human Embryos and Fetuses, the largest collection of human embryos worldwide, was initiated in the 1960s, and the Congenital Anomaly Research Center of Kyoto University was established in 1975 for long-term storage of the collection and for the promotion of research into human embryonic and fetal development. Currently, the Kyoto Collection comprises approximately 45,000 specimens of human embryonic or fetal development and is renowned for the following unique characteristics: (1) the collection is considered to represent the total population of fetal specimens nationwide in Japan, (2) it comprises a large number of specimens with a variety of external malformations, and (3) for most specimens there are clinical and epidemiological data from the mothers and the pregnancies concerned. Therefore, the specimens have been used extensively for morphological studies and could potentially be used for epidemiological analysis. Recently, several new approaches such as DNA extraction from formalin-fixed specimens or geometric morphometrics have been adopted and it is to be expected that further technological advances will facilitate new studies on the specimens of the Kyoto Collection as well as of other human embryo collections worldwide. Permanent preservation of the Kyoto Collection is, therefore, of paramount importance so that it will continue to contribute to human embryological studies in the future.

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Imaging of a Large Collection of Human Embryo Using a Super-Parallel MR Microscope

Cited by 46 publications

References 7 publications

Embryonic Liver Morphology and Morphometry by Magnetic Resonance Microscopic Imaging

Embryonic Liver Morphology and Morphometry by Magnetic Resonance Microscopic Imaging

Three-dimensional observation of the mouse embryo by micro-computed tomography: composition of the trigeminal ganglion

The Kyoto Collection of Human Embryos and Fetuses: History and Recent Advancements in Modern Methods

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