The Kyoto Collection of Human Embryos and Fetuses: History and Recent Advancements in Modern Methods

Yamaguchi, Yasushi; Yamada, Shigehito

doi:10.1159/000490672

Cited by 47 publications

(51 citation statements)

References 13 publications

(14 reference statements)

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“…Fourteen Japanese human embryos at CS20 (n = 4) and CS21 (n = 10) (Crown‐Rump length [CRL]: 16.5‐24.1 mm) were selected from the Kyoto Collection at the Congenital Anomaly Research Center of Kyoto University . The Kyoto Collection is one of the largest working collections of human embryos.…”

Section: Methodsmentioning

confidence: 99%

Vesicular swelling in the cervical region with lymph sac formation in human embryos

Ohga

Sakamoto

Yamada

et al. 2019

Congenital Anomalies

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Vesicular swelling in the cervical region (VSC) is occasionally observed among human embryos around Carnegie stage (CS) 21. However, its mechanism and significance in fetal development are unclear. The present study aimed to analyze the relation of development of VSC with jugular lymph sac (JLS) formation. Serial histological sections that were digitalized from 14 embryos at CS20 and CS21 stored at the Kyoto Collection were used for the analysis. Subcutaneous edema and enlargement of the subarachnoid space were found to cause VSC. No obvious abnormalities in cranial regions that may be related to the VSC were detected on histological sections. Three‐dimensional reconstructions revealed the following: (a) the JLS was located bilaterally at the levels between the first and fourth cervical vertebrae; (b) the JLS was pyramidal in shape; and (c) no severe deformity and/or malformation was found in all samples. The JLS was not connected to the subcutaneous tissue and subarachnoid space in all samples. The mean volume of the JLS increased nine‐times from CS20 (0.02 mm3 in VSC [−] group) to CS21 (0.18 mm3 in VSC [−] group). The mean volume of the JLS was comparable between the VSC [−] and VSC (+) groups at both CS20 and CS21. A moderate correlation was observed between VSCd and the mean volume of the JLS in both groups at CS20 (R2 = 0.75) and CS21 (R2 = 0.56). In conclusion, the dynamics of the lymphatic system at the cervical region may contribute to VSC observed around CS21. © 2019 Japanese Teratology Society

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Section: Methodsmentioning

confidence: 99%

Vesicular swelling in the cervical region with lymph sac formation in human embryos

Ohga

Sakamoto

Yamada

et al. 2019

Congenital Anomalies

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“…This study was approved by The Committee of Medical Ethics of Kyoto University Graduate School of Medicine, Kyoto, Japan (E986, R0316, R0989). Approximately 44 000 human embryos comprising the Kyoto Collection are stored at the Congenital Anomaly Research Center of Kyoto University . The specimens were typically donated to the Kyoto Collection following pregnancy termination during the first trimester for socioeconomic reasons, under the Maternity Protection Law in Japan.…”

Section: Methodsmentioning

confidence: 99%

“…Approximately 44 000 human embryos comprising the Kyoto Collection are stored at the Congenital Anomaly Research Center of Kyoto University. [20][21][22] The specimens were typically donated to the Kyoto Collection following pregnancy termination during the first trimester for socioeconomic reasons, under the Maternity Protection Law in Japan. Some specimens (~20%) represent undamaged, well-preserved embryos.…”

Section: Human Embryo Specimensmentioning

confidence: 99%

Human embryonic ribs all progress through common morphological forms irrespective of their position on the axis

Matsubayashi

Okuno

Fujii

et al. 2019

Developmental Dynamics

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Background We aimed to analyze the morphogenesis of all ribs from 1st to 12th rib pairs plus vertebrae to compare their differences and features according to the position along the cranial‐caudal axis during the human embryonic period. Results Rib pair formation was analyzed using high‐resolution digitalized imaging data (n = 29) between Carnegie stage (CS) 18 and CS23 (corresponding to ED13‐14 in mouse; HH29‐35 in chick). A total of 348 rib pairs, from 1st to 12th rib pairs of each sample were subjected to Procrustes and principal component (PC) analyses. PC1 and PC2 accounted for 76.3% and 16.4% (total 92.7%) of the total variance, respectively, indicating that two components mainly accounted for the change in shape. The distribution of PC1 and PC2 values for each rib showed a “fishhook‐like shape” upon fitting to a quartic equation. PC1 and PC2 value position for each rib pair moved along the fitted curve according to the development. Thus, the change in PC1 and PC2 could be expressed by a single parameter using a fitted curve as a linear scale for shape. Conclusion Human embryonic ribs all progress through common morphological forms irrespective of their position on the axis.

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“…In this study, therefore, we described a 3-D reconstruction of the shoulder girdle formation and positioning including the clavicle and scapula and its relationship to different landmarks in the body. [21][22][23] were used for this study (Table 1). These specimens were measured, examined, and staged according to the criteria proposed by O'Rahilly and Müller [24].…”

Section: Introductionmentioning

confidence: 99%

Shoulder girdle formation and positioning during embryonic and early fetal human development

Tanaka¹,

Sakamoto²,

Kanahashi³

et al. 2020

PLoS ONE

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Positional information on the shoulder girdle (the clavicle and scapula) is important for a better understanding of the function of the upper limb in the locomotive system as well as its associated disease pathogenesis. However, such data are limited except for information on the axial position of the scapula. Here, we describe a three-dimensional reconstruction of the shoulder girdle including the clavicle and scapula, and its relationship to different landmarks in the body. Thirty-six human fetal specimens (crown-rump length range: 7.6-225 mm) from the Kyoto Collection were used for this study. The morphogenesis and three-dimensional position of the shoulder girdle were analyzed with phase-contrast X-ray computed tomography and magnetic resonance imaging. We first detected the scapula body along with the coracoid and humeral head at Carnegie stage 18; however, the connection between the body and coracoid was not confirmed at this stage. During development, all landmarks on the shoulder girdle remained at the same axial position except for the inferior angle, which implies that the scapula enlarged in the caudal direction and reached the adult axial position in the fetal period. The scapula body was rotated internally and in the upward direction at the initiation of morphogenesis, but in the fetal period the scapula body was different than that in the adult position. The shoulder girdle was located at the ventral side of the vertebrae at the time of initial morphogenesis, but changed its position to the lateral side of the vertebrae in the late embryonic and fetal periods. Such a unique position of the shoulder girdle may contribute to the stage-specific posture of the upper limb. Adequate internal and upward rotation of the scapula could help in reducing the shoulder width, thereby facilitating childbirth. The data presented in this study can be used as normal morphometric references for shoulder girdle evaluations in the embryonic and fetal periods.

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The Kyoto Collection of Human Embryos and Fetuses: History and Recent Advancements in Modern Methods

Cited by 47 publications

References 13 publications

Vesicular swelling in the cervical region with lymph sac formation in human embryos

Vesicular swelling in the cervical region with lymph sac formation in human embryos

Human embryonic ribs all progress through common morphological forms irrespective of their position on the axis

Shoulder girdle formation and positioning during embryonic and early fetal human development

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