Imaging human epithelial properties with polarized light-scattering spectroscopy

Gurjar, Rohit; Backman, Vadim; Perelman, Lev T.; Georgakoudi, Irene; Badizadegan, Kamran; Itzkan, Irving; Dasari, Ramachandra R.; Feld, Michael S.

doi:10.1038/nm1101-1245

Cited by 365 publications

(232 citation statements)

References 14 publications

Supporting

Mentioning

225

Contrasting

Unclassified

Order By: Relevance

“…Among them, Mie scattering theory for spherical scatterers is widely employed for tissue scattering analysis [11,46,47], which for a single scattering event manifests a Mueller matrix in the form of a diattenuated retarder, represented by M DR [43] …”

Section: Polarized Light and Biological Tissuementioning

confidence: 99%

Mueller polarimetric imaging for surgical and diagnostic applications: a review

2017

View full text Add to dashboard Cite

Polarization is a fundamental property of light and a powerful sensing tool that has been applied to many areas. A Mueller matrix is a complete mathematical description of the polarization characteristics of objects that interact with light, and is known as a transfer function of Stokes vectors which characterise the state of polarization of light. Mueller polarimetric imaging measures Mueller matrices over a field of view and thus allows for visualising the polarization characteristics of the objects. It has emerged as a promising technique in recent years for tissue imaging, improving image contrast and providing a unique perspective to reveal additional information that cannot be resolved by other optical imaging modalities. This review introduces the basis of the Stokes-Mueller formulism, interpretation methods of Mueller matrices into fundamental polarization properties, polarization properties of biological tissues, and considerations in the construction of Mueller polarimetric imaging devices for surgical and diagnostic applications, including primary configurations, optimization procedures, calibration methods as well as the instrument polarization properties of several widelyused biomedical optical devices. The paper also reviews recent progress in Mueller polarimetric endoscopes and fibre Mueller polarimeters, followed by the future outlook in applying the technique to surgery and diagnostics. Tissue polarization properties convey morphological, micro-structural and compositional information of tissue with great potential for label free characterization of tissue pathological changes. Recent progress in tissue polarimetric imaging and polarization resolved endoscopy paved the way for translation of polarimetric imaging to surgery and tissue diagnosis.

show abstract

Section: Polarized Light and Biological Tissuementioning

confidence: 99%

Mueller polarimetric imaging for surgical and diagnostic applications: a review

2017

View full text Add to dashboard Cite

show abstract

“…On a cellular level, the refractive index has been shown to be sensitive to nuclear changes occurring as a result of cell mitosis [6], application of a contrast agent [7], and dysplasia [8]. Refractive index differences have also been exploited in the selective delivery of laser energy to tissue [9].…”

Section: Introductionmentioning

confidence: 99%

Refractive index tomography of turbid media by bifocal optical coherence refractometry

Zvyagin

Alexandrov²,

Silva

et al. 2004

SPIE Proceedings

View full text Add to dashboard Cite

Abstract:We demonstrate tomographic imaging of the refractive index of turbid media using bifocal optical coherence refractometry (BOCR). The technique, which is a variant of optical coherence tomography, is based on the measurement of the optical pathlength difference between two foci simultaneously present in a medium of interest. We describe a new method to axially shift the bifocal optical pathlength that avoids the need to physically relocate the objective lens or the sample during an axial scan, and present an experimental realization based on an adaptive liquid-crystal lens. We present experimental results, including video clips, which demonstrate refractive index tomography of a range of turbid liquid phantoms, as well as of human skin in vivo.

show abstract

“…A further advance also under study by Gurjar et al is the development of a wide-field spectral technique, based on LSS, that can image the entire cervix and indicate areas suspected of being cervical neoplasia. 37 The strategy is described as the spectral version of aceto-whitening because it provides a means with which to identify areas of potential abnormality. Areas identified as suspect then will be examined in more detail with the TMS optical fiber contact probe, which can sample a field measuring 1 mm in greatest dimension.…”

Section: Trimodal Spectroscopymentioning

confidence: 99%