Imaging H2O2 Microdomains in Receptor Tyrosine Kinases Signaling

Mishina, Natalia M.; Markvicheva, Kseniya N.; Fradkov, Arkady F.; Zagaynova, Elena V.; Schultz, Carsten; Lukyanov, Sergey; Belousov, Vsevolod V.

doi:10.1016/b978-0-12-405883-5.00011-9

Cited by 17 publications

(8 citation statements)

References 15 publications

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“…While it is uncontested that catalase is located in the peroxisomal matrix, other locations of catalase activity are also relevant, such as the mitochondria [104] and even the extracellular space [105]. Using D-amino acid oxidase as genetically encoded producer of H 2 O 2 , in conjunction with the fused HyPer probe, it becomes possible to examine intracellular H 2 O 2 dynamics quantitatively [106]·H 2 O 2 microdomains in receptor tyrosine kinase signaling are now amenable to imaging [107].…”

Section: Discussionmentioning

confidence: 99%

Hydrogen peroxide as a central redox signaling molecule in physiological oxidative stress: Oxidative eustress

2017

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Hydrogen peroxide emerged as major redox metabolite operative in redox sensing, signaling and redox regulation. Generation, transport and capture of H2O2 in biological settings as well as their biological consequences can now be addressed. The present overview focuses on recent progress on metabolic sources and sinks of H2O2 and on the role of H2O2 in redox signaling under physiological conditions (1–10 nM), denoted as oxidative eustress. Higher concentrations lead to adaptive stress responses via master switches such as Nrf2/Keap1 or NF-κB. Supraphysiological concentrations of H2O2 (>100 nM) lead to damage of biomolecules, denoted as oxidative distress. Three questions are addressed: How can H2O2 be assayed in the biological setting? What are the metabolic sources and sinks of H2O2? What is the role of H2O2 in redox signaling and oxidative stress?

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Section: Discussionmentioning

confidence: 99%

Hydrogen peroxide as a central redox signaling molecule in physiological oxidative stress: Oxidative eustress

2017

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“…Images were analysed as described by Mishina et al 61 . Briefly, images were smoothed using a Gaussian filter, and pixels containing the biosensor signal separated from the background using Otsu’s method 62 .…”

Section: Methodsmentioning

confidence: 99%

Photosynthesis-dependent H2O2 transfer from chloroplasts to nuclei provides a high-light signalling mechanism

et al. 2017

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Chloroplasts communicate information by signalling to nuclei during acclimation to fluctuating light. Several potential operating signals originating from chloroplasts have been proposed, but none have been shown to move to nuclei to modulate gene expression. One proposed signal is hydrogen peroxide (H2O2) produced by chloroplasts in a light-dependent manner. Using HyPer2, a genetically encoded fluorescent H2O2 sensor, we show that in photosynthetic Nicotiana benthamiana epidermal cells, exposure to high light increases H2O2 production in chloroplast stroma, cytosol and nuclei. Critically, over-expression of stromal ascorbate peroxidase (H2O2 scavenger) or treatment with DCMU (photosynthesis inhibitor) attenuates nuclear H2O2 accumulation and high light-responsive gene expression. Cytosolic ascorbate peroxidase over-expression has little effect on nuclear H2O2 accumulation and high light-responsive gene expression. This is because the H2O2 derives from a sub-population of chloroplasts closely associated with nuclei. Therefore, direct H2O2 transfer from chloroplasts to nuclei, avoiding the cytosol, enables photosynthetic control over gene expression.

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“…The images were analysed according to the method described by Mishina et al (). Briefly, images were smoothed using a Gaussian filter, and pixels containing the biosensor signal were separated from the background according to Otsu's method (Otsu, ).…”

Section: Methodsmentioning

confidence: 99%

Cadmium induces reactive oxygen species‐dependent pexophagy in Arabidopsis leaves

Calero-Muñoz

Expósito-Rodríguez

Collado-Arenal

et al. 2019

Plant Cell & Environment

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Cadmium treatment induces transient peroxisome proliferation in Arabidopsis leaves.To determine whether this process is regulated by pexophagy and to identify the mechanisms involved, we analysed time course-dependent changes in ATG8, an autophagy marker, and the accumulation of peroxisomal marker PEX14a. After 3 hr of Cd exposure, the transcript levels of ATG8h, ATG8c, a, and i were slightly upregulated and then returned to normal. ATG8 protein levels also increased after 3 hr of Cd treatment, although an opposite pattern was observed in PEX14.Arabidopsis lines expressing GFP-ATG8a and CFP-SKL enabled us to demonstrate the presence of pexophagic processes in leaves. The Cd-dependent induction of pexophagy was demonstrated by the accumulation of peroxisomes in autophagy gene (ATG)-related Arabidopsis knockout mutants atg5 and atg7. We show that ATG8a colocalizes with catalase and NBR1 in the electron-dense peroxisomal core, thus suggesting that NBR1 may be an autophagic receptor for peroxisomes, with catalase being possibly involved in targeting pexophagy. Protein carbonylation and peroxisomal redox state suggest that protein oxidation may trigger pexophagy. Cathepsine B, legumain, and caspase 6 may also be involved in the regulation of pexophagy. Our results suggest that pexophagy could be an important step in rapid cell responses to cadmium.

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Imaging H2O2 Microdomains in Receptor Tyrosine Kinases Signaling

Cited by 17 publications

References 15 publications

Hydrogen peroxide as a central redox signaling molecule in physiological oxidative stress: Oxidative eustress

Hydrogen peroxide as a central redox signaling molecule in physiological oxidative stress: Oxidative eustress

Photosynthesis-dependent H2O2 transfer from chloroplasts to nuclei provides a high-light signalling mechanism

Cadmium induces reactive oxygen species‐dependent pexophagy in Arabidopsis leaves

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