Imaging behind opaque obstacle: a potential method for guided in vitro needle placement

Abstract

Abstract:We report a simple real time optical imaging concept using an axicon lens to image the object kept behind opaque obstacles in free space. The proposed concept underlines the importance and advantages of using an axicon lens compared to a conventional lens to image behind the obstacle. The potential of this imaging concept is demonstrated by imaging the insertion of surgical needle in biological specimen in real time, without blocking the field of view. It is envisaged that this proposed concepts and methodology can make a telling impact in a wide variety of areas especially for diagnostics, therapeutics and microscopy applications.

Breaking diffraction limit of far-field imaging via structured illumination Bessel beam microscope (SIBM)

“…As described in Appendix B, the PSF of the imaging system can be narrowed by selecting an axicon lens with a higher physical angle (α). Importantly, an image captured using an axicon lens is known to be affected by swirl (haze) [17,20]. Higher physical angles of the axicon lens will result in an increased image swirl.…”

Plasmonic random laser enabled artefact-free wide-field fluorescence bioimaging: uncovering finer cellular features

“…Fluorescence imaging is an indispensable tool in life science research and medical diagnostics owing to its contributions to understanding cell physiology, transport characteristics, and interactions of ions, proteins, and other macromolecules. [1][2][3][4][5][6] Lasers, with their intense, narrow-band, and tunable emission, have paved the way for a variety of novel applications based on uorescence imaging. However, the high coherence of the laser leads to the interference of light scattered from optical surfaces, dust particles, inherent imperfections in the system and the sample surface.…”

Indirect gonioscopy system for imaging iridocorneal angle of eye

“…Optics and photonics engineering play a very vital role in the areas such as imaging [1][2][3][4][5][6] and translational medicine [7][8][9][10][11][12]. In recent years, specifically in vision science there have been many advanced medical diagnosis tests for a variety of ocular diseases.…”