Plasmonic random laser enabled artefact-free wide-field fluorescence bioimaging: uncovering finer cellular features

Radhakrishnan, Gayathri; Sandeep, C. S. Suchand; Gummaluri, Venkata Siva; Asik, R. Mohammed; Gulyás, Balázs; Vijayan, C.; Matham, Murukeshan Vadakke

doi:10.1039/d1na00866h

Cited by 10 publications

(9 citation statements)

References 59 publications

(62 reference statements)

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“…This unique combination is absent in other light sources (e.g., conventional lasers, thermal light sources, or LEDs); see Figure 9 for a comparison of different illumination sources. Figure 10 shows a comparison of the speckle-free imaging capability of a random laser with that of an LED and a conventional laser source [141]. These images clearly show that random lasers generate speckle-free images, unlike conventional laser sources.…”

Section: Imaging Applicationsmentioning

confidence: 98%

Lasing from Micro- and Nano-Scale Photonic Disordered Structures for Biomedical Applications

Gayathri,

Suchand Sandeep,

Vijayan

et al. 2023

Nanomaterials

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A disordered photonic medium is one in which scatterers are distributed randomly. Light entering such media experiences multiple scattering events, resulting in a “random walk”-like propagation. Micro- and nano-scale structured disordered photonic media offer platforms for enhanced light–matter interaction, and in the presence of an appropriate gain medium, coherence-tunable, quasi-monochromatic lasing emission known as random lasing can be obtained. This paper discusses the fundamental physics of light propagation in micro- and nano-scale disordered structures leading to the random lasing phenomenon and related aspects. It then provides a state-of-the-art review of this topic, with special attention to recent advancements of such random lasers and their potential biomedical imaging and biosensing applications.

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Section: Imaging Applicationsmentioning

confidence: 98%

Lasing from Micro- and Nano-Scale Photonic Disordered Structures for Biomedical Applications

Gayathri,

Suchand Sandeep,

Vijayan

et al. 2023

Nanomaterials

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“…Random lasing is an unconventional lasing method, which operate without the traditional mirror cavities and relies on multiple scattering in a disordered medium to amplify light [15][16][17][18] . Tissues, being a disordered medium is capable of light trapping and exhibit random lasing in the presence of an appropriate gain medium.…”

Section: Introductionmentioning

confidence: 99%

Tumour polyp detection using random lasing

Gayathri,

Suchand Sandeep,

Vijayan

et al. 2024

Biomedical Applications of Light Scattering XIV

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Tumors arise from the uncontrolled growth of cells and can be benign or cancerous. The size of the tumor is one of the key factors in assessing its malignancy potential. Generally, larger tumor polyp tends to have higher risk of developing into a cancer. Smaller polyps often start benign but some of it evolve over time to adenomatous or cancerous and extend to other parts of the body. To mitigate this risk, even smaller polyps are usually removed if found during screening. However, the detection of small polyps, especially flat or sessile types, remains a challenge. Advanced techniques are being developed to identify early-stage tumors by studying biomechanical, biochemical, and morphological changes. Tumor progression alters the viscoelasticity, local refractive index, and surface roughness, increasing tissue disorder both structurally and optically. This disorder can localize light through multiple scattering and can be utilized to provide cavity feedback for lasing emission called 'random lasing'. In this work, we simulate a tumor polyp growth in a phantom tissue impregnated with a gain medium and investigate the resulting random lasing emission. We find that the emission properties such as the intensity, lasing threshold, emission wavelength, and linewidth are all influenced by the presence of the polyp and this technique could precisely identify even polyps of size ~ millimeters. Overall, this research showcases the potential of random lasing to investigate disorder-induced changes for early and sensitive detection of tumor and identification of tissue abnormalities.

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“…The high photon degeneracy and low spatial coherence of random lasers make them appealing for imaging applications. Recent research reports show that random lasers could generate speckle-free images even in highly scattering environments [29][30][31]. Random lasers have been used for high-contrast in vitro dental imaging in the backscattering configuration to image through turbid media, for imaging dynamic phenomena like the blood flow patterns in mouse ear skin, and for other imaging applications [32].…”

Section: Introductionmentioning

confidence: 99%

Random Lasing for Bimodal Imaging and Detection of Tumor

Gayathri,

Suchand Sandeep,

Vijayan

et al. 2023

Biosensors

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The interaction of light with biological tissues is an intriguing area of research that has led to the development of numerous techniques and technologies. The randomness inherent in biological tissues can trap light through multiple scattering events and provide optical feedback to generate random lasing emission. The emerging random lasing signals carry sensitive information about the scattering dynamics of the medium, which can help in identifying abnormalities in tissues, while simultaneously functioning as an illumination source for imaging. The early detection and imaging of tumor regions are crucial for the successful treatment of cancer, which is one of the major causes of mortality worldwide. In this paper, a bimodal spectroscopic and imaging system, capable of identifying and imaging tumor polyps as small as 1 mm2, is proposed and illustrated using a phantom sample for the early diagnosis of tumor growth. The far-field imaging capabilities of the developed system can enable non-contact in vivo inspections. The integration of random lasing principles with sensing and imaging modalities has the potential to provide an efficient, minimally invasive, and cost-effective means of early detection and treatment of various diseases, including cancer.

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Plasmonic random laser enabled artefact-free wide-field fluorescence bioimaging: uncovering finer cellular features

Abstract: Narrow bandwidth, high brightness, and spectral tunability are the unique properties of lasers that make them extremely desirable for fluorescence imaging applications. However, due to the high spatial coherence, conventional...

Cited by 10 publications

References 59 publications

Lasing from Micro- and Nano-Scale Photonic Disordered Structures for Biomedical Applications

Lasing from Micro- and Nano-Scale Photonic Disordered Structures for Biomedical Applications

Tumour polyp detection using random lasing

Random Lasing for Bimodal Imaging and Detection of Tumor

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