Image windowing mitigates edge effects in Differential Dynamic Microscopy

Giavazzi, Fabio; Edera, Paolo; Lu, Peter J.; Cerbino, Roberto

doi:10.1140/epje/i2017-11587-3

Cited by 24 publications

(23 citation statements)

References 24 publications

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“…Note that the scattering functions are shown for a q-value of 4.65 mm À1 , which is an intermediate value where the ISF is not affected by noise or by drifting of the particles out of the field of view (see ref. 52 and ESI, † Fig. S3).…”

Section: Effect Of Crosslinking On Particle Diffusivity In Hyaluronanmentioning

confidence: 99%

Particle diffusion in extracellular hydrogels

et al. 2020

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Section: Effect Of Crosslinking On Particle Diffusivity In Hyaluronanmentioning

confidence: 99%

Particle diffusion in extracellular hydrogels

et al. 2020

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“…While performing the numerical Fourier transform, care has to be taken to avoid imaged inhomogeneities leading to spectral leakage. 39 Assuming a stationary and isotropic signal, the signal statistics and hence the power spectrum are independent of time t and the direction of the scattering vector Q. To improve statistics, thus, a temporal average over t and an azimuthal average over the angle φ in the Q plane are performed and indicated by t,φ .…”

Section: Determination Of the Steady-shear Viscosity In A Ddm Experimentsmentioning

confidence: 99%

Microliter viscometry using a bright-field microscope: η-DDM

et al. 2018

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The rheological properties of a medium can be inferred from the Brownian motion of colloidal tracer particles using the microrheology procedure. The tracer motion can be characterized by the mean-squared displacement (MSD). It can be calculated from the intermediate scattering function determined by Differential Dynamic Microscopy (DDM). Here we show that DDM together with the empirical Cox-Merz rule is particularly suited to measure the steady-shear viscosity, i.e. the viscosity towards zero frequency, due to its ability to provide reliable information on long time and length scales and hence small frequencies. This method, η-DDM, is tested and illustrated using three different systems: Newtonian fluids (glycerol-water mixtures), colloidal suspensions (protein samples) and a viscoelastic polymer solution (aqueous poly(ethylene oxide) solution). These tests show that common lab equipment, namely a bright-field optical microscope, can be used as a convenient and reliable microliter viscometer. Because η-DDM requires much smaller sample volumes than classical rheometry, only a few microliters, it is particularly useful for biological and soft matter systems.

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“…It has been recently shown that multiplying the images with a windowing function before performing the Fourier transform operation removes the artifacts due to the finite image size and improves the determination of D(q,t), especially for those q for which the signal is comparable or smaller than the noise [46]. We thus apply this algorithm in our analysis.…”

Section: E Differential Dynamic Microscopymentioning

confidence: 99%

Differential dynamic microscopy microrheology of soft materials: A tracking-free determination of the frequency-dependent loss and storage moduli

Edera

Bergamini

Trappe

et al. 2017

Phys. Rev. Materials

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Particle-tracking microrheology (PT-μr) exploits the thermal motion of embedded particles to probe the local mechanical properties of soft materials. Despite its appealing conceptual simplicity, PT-μr requires calibration procedures and operating assumptions that constitute a practical barrier to its wider application. Here we demonstrate differential dynamic microscopy microrheology (DDM-μr), a tracking-free approach based on the multiscale, temporal correlation study of the image intensity fluctuations that are observed in microscopy experiments as a consequence of the translational and rotational motion of the tracers. We show that the mechanical moduli of an arbitrary sample are determined correctly over a wide frequency range provided that the standard DDM analysis is reinforced with an iterative, self-consistent procedure that fully exploits the multiscale information made available by DDM. Our approach to DDM-μr does not require any prior calibration, is in agreement with both traditional rheology and diffusing wave spectroscopy microrheology, and works in conditions where PT-μr fails, providing thus an operationally simple, calibration-free probe of soft materials.

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Image windowing mitigates edge effects in Differential Dynamic Microscopy

Cited by 24 publications

References 24 publications

Particle diffusion in extracellular hydrogels

Particle diffusion in extracellular hydrogels

Microliter viscometry using a bright-field microscope: η-DDM

Differential dynamic microscopy microrheology of soft materials: A tracking-free determination of the frequency-dependent loss and storage moduli

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