Image of the Micro-Computed Tomography and Atomic-Force Microscopy of Bone in Osteoporosis Animal Model

Abstract

Osteoporosis, a major public health problem, is characterised by a reduction in and deterioration of bone tissue microarchitecture, with a consequent increase in bone frailty and susceptibility to fractures. We performed ex vivo micro-computed tomography (micro-CT) and atomic-force microscopy (AFM) between normal and surgically ovariectomized animals (OVX). The tibial architecture was investigated using maximum intensity projection images. Micro-CT analysis of the tibia metaphysis was used to calculate the trabecular spacing (Tb.Sp). Following OVX surgery, the rats exhibited increased Tb.Sp. Between the bundles, there are holes in the fabric with a diameter in the range of 3 to 5 μm in normal tibia. The diameter of hole in the osteoporosis tibia is the range of 5 to 10 μm, and its area increased than normal tibia. AFM can be used for the inspection of bone surfaces and offers the possibility of quantification of local geometries, such as pit size, collagen fibril diameters, or collagen ultrastructure. Our results suggest that AFM will surely become one of the most important tools in the realm of medical research for imaging such as bone and tissues.

Influence of Prednisolone and Alendronate on the de novo Mineralization of Zebrafish Caudal Fin

“…Osteoporotic‐like bones are characterized by the mineral resorption caused by increased osteoclasts activity that often reconfigure bone's microarchitecture. ( 3,13,46 ) Following the overall trend in this research, the proximal areas verified by AFM revealed a different appearance from those obtained from distal sites. The frequent and low amplitude peaks images found in the bones extracted from the proximal regions are characteristic from highly mineralized tissues.…”

“…(12) The use of animal models has therefore been proposed. (13,14) anaesthetized individually in a 200 mL solution containing 70 ppm of Tricaine (MS-222;Sigma Aldrich; Missouri, MO, USA), adjusted to pH ~7.2 (± 0.1) with sodium hydroxide (NaOH), 130 µL of isoflurane (Alfa Aesar; Loughborough, Leicestershire, UK) and ethanol (isoflurane:ethanol = 1:9), as described elsewhere. (32) The anaesthetized fish were placed carefully onto a cell and tissue culture disk (Biofil; Kaohsiung, Taiwan) and the tiles images of the bright field (T-PMT-T3) and fluorescent light (Cy3-T3) of the caudal fin bony rays were taken with an inverted confocal microscope (LSM 800; Carl Zeiss, Oberkochen, Germany).…”

Development of a Method for Analyzing Tomographic Images of Bone Structures

“…High-resolution MRI (HR-MRI) techniques can provide three-dimensional information about cancellous bone in peripheral regions with a resolution of the order of trabecular dimensions (in-plane resolution: ~ 110 μm, section thickness: ~ 300 μm) [7][8][9][10]. Recent advances also allow imaging of the proximal femur, a major area of clinical interest, although the signal-to-noise ratio (SNR) and resolution (in-plane resolution: ~ 250 μm, slice thickness: ~ 500 μm) are lower than peripheral areas [11].…”