Illustration of the Genetic Differences in Ozone Sensitivity Between the Varieties Nicotiana tabacum Bel W₃ and Bel B Using Various Plant Systems

Abstract

The ozone‐sensitive tobacco variety Bel W3 was compared with the tolerant cv. Bel B using amphidiploid and amphihaploid genotypes of both. In search of the first genotypical differences, their reaction to acute ozone treatments was investigated with systems of decreasing degree of complexity: whole plants, grown under field, greenhouse and sterile conditions, excised tissues, calli, CCP, MCP and subcellular reactions. It was common to all systems that a fumigation, which clearly exceeds the threshold of the most tolerant material, led to equal reactions in all genotypes in respect of visible injury and membrane leaching. With whole plants and leaf discs growing conditions were found to influence the ozone threshold more than the genotype. Because the most resistant field‐grown plants vary widely in their reaction, only sterile or greenhouse grown genotypes were compared. With the exception of whole sterile plants (no genotypical threshold differences), amphihaploids were more susceptible to ozone than their respective amphidiploids as to threshold and sensitivity spectra in all systems investigated. Higher ozone thresholds were detected for Bel B in all systems with one exception: MCP exhibited a lower threshold but also a lower degree of damage in the first buffer range of the sensitivity spectrum than those of Bel W3. Post‐fumigation starch accumulation in mesophyll chloroplasts was the most prominent subcellular ozone reaction.

Effects of ozone on the plasma membrane proteins in Arabidopsis thaliana (L.) leaves

“…3) ptesent in control plants incteased after ozone treatment by regulated upon ozone exposure, and do not follow the overall reduction in plasmalemma protein eontent, suggests that they are closely linked to ozone response and assoeiated with defence mechanisms. Such a conclusion is supported by the close relationship between ozone sensitivity and membrane traits demonstrated by Stadtler & Ziegler (1993).…”

“…Sttess-induced plasma tnembratie changes may strongly perturb different cellular functions, but they can also play an important role in adaptation to stress and stress defence. In the case of ozone-induced sttess, Stadtler & Ziegler (1993) detnonstrated that variational resistance of tobacco plants to this pollutant otiginates mainly frotn membrane traits. Existing studies on the effect of ozone on plasma membrane proteins tnainly concetn functioning of the proteins involved in ttansport processes.…”

Sensitive Detection of the Leaching‐out from Membrane‐surrounded Compartments: a New Approach Explored with Cells and Tissues of Nicotiana tabacum L.

“…During development of this test, all experiments were done at least twice, but all results, unless stated otherwise, presented here were drawn from a single experiment. The results, obtained with the fully established test summarizing a statistical number of single experiments are presented separately (Stadtler and Ziegler, 1993). A.…”

Wood Distillate Mitigates Ozone-Induced Visible and Photosynthetic Plant Damage: Evidence from Ozone-Sensitive Tobacco (Nicotiana tabacum L.) BelW3

“…A field experiment was carried out in the urban area of Parma. A comparative analysis of the physiological results was made, comparing results obtained on BelW3 with those of BelB, i.e., tobacco plants known for their resistance to this pollutant [28]. The research also aimed to investigate the persistence of the protective effect (if any), by carrying out measurements three weeks after the end of treatments.…”