Effects of ozone on the plasma membrane proteins in Arabidopsis thaliana (L.) leaves

Abstract

The protein pattern of leaf plasma membranes from Arabidopsis thaliana (L.) Landsberg erecta was analysed in order to detect changes induced by acute short‐term ozone treatment. Plasma membranes were isolated 0, 3 and 8 h after the end of a 2 h fumigation of the plants with 500 nmol mol−1 of O3. Proteins extracted from plasma membranes were separated by high‐performance two‐dimensional polyacrylamide gel electrophoresis. Eight hours after the end of fumigation, one new protein appeared and the amounts of two other proteins increased significantly. The reported study is a first step towards the identification of plasmalemma proteins altered by ozone and to a more detailed characterization of structural changes occurring in the plasma membrane after ozone exposure.

Gene expression patterns of trembling aspen trees following long‐term exposure to interacting elevated CO2 and tropospheric O3

“…Photosynthetic protein mRNAs (Conklin & Last, 1995) and polyamines (Scalet et al ., 1994) are also commonly up‐regulated by O 3 . Ozone alters the thylakoid and chloroplast membranes in turn, decreasing photosynthetic capacity (Tognini et al ., 1997) and weakening plasma membranes (Tokarska‐Schlattner et al ., 1997).…”

Effects of air pollutants on proton and sucrose transport at the plasma membrane of Ricinus communis

“…A similar result was observed after in vivo treatment of whole tissue with SO 2 and O 3 prior to membrane isolation. Tokarska-Schlattner et al (1997) have recently reported that O 3 fumigation of Arabidopsis leaves results in an increase in some plasma membrane proteins as shown by two-dimensional gel electrophoresis. However, the sizes of these proteins do not correspond with that of the H + -ATPase.…”