Illustrated histopathological features of fatal dengue cases in Colombia

Rivera, Jorge; Rengifo, Aura Caterine; Parra, Édgar; Castellanos, Jaime E.; Caldas, María Leonor

doi:10.7705/biomedica.5016

Cited by 4 publications

(6 citation statements)

References 21 publications

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“…Dengue symptoms range from a mild flu-like syndrome to a severe and, sometimes, fatal disease, classified by the World Health Organization (WHO) as severe dengue (SD) which may affect multiple organ systems [12]. The DENV has been detected in a number of organs [13][14][15][16] and, although the liver is the most commonly affected one [17,18], gastrointestinal, hepatic, respiratory, cardiac, neurological and renal manifestations during DENV infection have already been reported [19][20][21][22][23][24][25][26].…”

Section: Introductionmentioning

confidence: 99%

Brazilian Dengue Virus Type 2-Associated Renal Involvement in a Murine Model: Outcomes after Infection by Two Lineages of the Asian/American Genotype

et al. 2021

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Dengue virus type 2 (DENV-2) is, traditionally, the most studied serotype due to its association with explosive outbreaks and severe cases. In Brazil, almost 20 years after the first introduction in the 1990s, a new lineage (Lineage II) of the DENV-2 Asian/American genotype emerged and caused an epidemic with severe cases and hospitalizations. Severe dengue includes multiple organ failure, and renal involvement can be potentially related to increased mortality. In order to better understand the role of DENV infection in renal injury, here we aimed to investigate the outcomes of infection with two distinct lineages of DENV-2 Asian/American genotype in the kidney of a murine model. BALB/c mice were infected with Lineages I and II and tissues were submitted to histopathology, immunohistochemistry, histomorphometry and ultrastructural analysis. Blood urea nitrogen (BUN) was detected in blood sample accessed by cardiac puncture. A tendency in kidney weight increase was observed in mice infected with both lineages, but urea levels, on average, were increased only in mice infected with Lineage II. The DENV antigen was detected in the tissue of mice infected with Lineage II and morphological changes were similar to those observed in human dengue cases. Furthermore, the parameters such as organ weight, urea levels and morphometric analysis, showed significant differences between the two lineages in the infected BALB/c, which was demonstrated to be a suitable experimental model for dengue pathophysiology studies in kidneys.

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Section: Introductionmentioning

confidence: 99%

Brazilian Dengue Virus Type 2-Associated Renal Involvement in a Murine Model: Outcomes after Infection by Two Lineages of the Asian/American Genotype

et al. 2021

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“…Kupffer cell hyperplasia and hypertrophy in YF have been widely documented in the scientific literature by several authors [7,100,120]. Hyperplasia and Kupffer cell destruction have been identified in most fatal DF cases [37,80,121,122]. Liver injury observed in hemorrhagic fevers, such as DF and YF, revealed that hepatocytes and Kupffer cells might be the target cells for viral replication [69,123].…”

Section: Discussionmentioning

confidence: 94%

“…In YF, macro-and microvesicular steatosis is a change commonly observed in infected patients, present in the three zones of the hepatic acinus, but it is predominant in Z2 [57,[77][78]. DF is associated with fatty liver degeneration [79,80]. Recently, Win et al reported findings in human liver samples from patients who died of dengue hemorrhagic fever (DHF), which showed extensive cell damage and microvesicular steatosis [37].…”

Section: Discussionmentioning

confidence: 99%

A Comparative Analysis of Human Hepatic Lesions in Dengue, Yellow Fever, and Chikungunya: Revisiting Histopathological Changes in the Light of Modern Knowledge of Cell Pathology

Ribeiro¹,

Falcão²,

Smith³

et al. 2023

Preprint

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Arboviruses such as yellow fever virus (YFV), dengue virus (DENV), and chikungunya virus (CHIKV) presenting wide global dissemination and the pathogenic profile developed in infected individuals, which develop from nonspecific clinical conditions to severe forms, characterized by the promotion of significant lesions in different organs of the harborer, culminating in multiple organ dysfunction. To characterize, quantify, and compare the patterns of histopathological alterations in human liver samples from patients with yellow fever (YF), dengue fever (DF), and chikungunya fever (CF). Analytical cross-sectional study by histopathological analysis with 70 samples of liver patients, collected from 2000 to 2017, with confirmed laboratory diagnosis who died due to infection and complications by the YF, DF, and CF. Of the histopathological findings in human liver samples there was a significant difference between the control and infection groups, with a predominance of alterations in the midzonal area of the three cases analyzed, among the arboviruses studied, the hepatic involvement in cases of YF showed greater intensity of histopathological changes. Among the alterations evaluated, cell swelling, microvesicular steatosis and apoptosis were classified as degree of tissue damage from severe to very severe. The pathological abnormalities associated with infection by YFV, DENV, and CHIKV showed predominance of changes in the midzonal area. We also noted that in among of the arboviruses studied, liver involvement in cases of YFV infection was more intense.

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“…In YF, macro- and microvesicular steatosis is a change commonly observed in infected patients, present in the three zones of the hepatic acinus but predominant in Z2 [ 57 , 61 , 62 ]. DF is associated with fatty liver degeneration [ 63 , 64 ]. Recently, Win et al [ 65 ] reported extensive cell damage and microvesicular steatosis in human liver samples from patients who died of dengue haemorrhagic fever (DHF) [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

“…According to Pan et al [ 83 ], the development of injurious mechanisms in Z2 of the hepatic acinus is characteristic of the pathogenesis of flavivirus infections. This pattern of injury in the midzonal area is attributed to the intrinsic characteristics of hepatocytes, which have a lower presence of oxygen and nutrients than Z1 [ 40 , 64 , 68 ]. In a study by Olímpio et al [ 62 ], lytic and coagulative necrosis were recurrent findings in the three hepatic zones of patients with YF; however, the highest frequency was observed in Z2.…”

Section: Discussionmentioning

confidence: 99%

Comparative Analysis of Human Hepatic Lesions in Dengue, Yellow Fever, and Chikungunya: Revisiting Histopathological Changes in the Light of Modern Knowledge of Cell Pathology

et al. 2023

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Arboviruses, such as yellow fever virus (YFV), dengue virus (DENV), and chikungunya virus (CHIKV), present wide global dissemination and a pathogenic profile developed in infected individuals, from non-specific clinical conditions to severe forms, characterised by the promotion of significant lesions in different organs of the harbourer, culminating in multiple organ dysfunction. An analytical cross-sectional study was carried out via the histopathological analysis of 70 samples of liver patients, collected between 2000 and 2017, with confirmed laboratory diagnoses, who died due to infection and complications due to yellow fever (YF), dengue fever (DF), and chikungunya fever (CF), to characterise, quantify, and compare the patterns of histopathological alterations in the liver between the samples. Of the histopathological findings in the human liver samples, there was a significant difference between the control and infection groups, with a predominance of alterations in the midzonal area of the three cases analysed. Hepatic involvement in cases of YF showed a greater intensity of histopathological changes. Among the alterations evaluated, cell swelling, microvesicular steatosis, and apoptosis were classified according to the degree of tissue damage from severe to very severe. Pathological abnormalities associated with YFV, DENV, and CHIKV infections showed a predominance of changes in the midzonal area. We also noted that, among the arboviruses studied, liver involvement in cases of YFV infection was more intense.

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Illustrated histopathological features of fatal dengue cases in Colombia

Cited by 4 publications

References 21 publications

Brazilian Dengue Virus Type 2-Associated Renal Involvement in a Murine Model: Outcomes after Infection by Two Lineages of the Asian/American Genotype

Brazilian Dengue Virus Type 2-Associated Renal Involvement in a Murine Model: Outcomes after Infection by Two Lineages of the Asian/American Genotype

A Comparative Analysis of Human Hepatic Lesions in Dengue, Yellow Fever, and Chikungunya: Revisiting Histopathological Changes in the Light of Modern Knowledge of Cell Pathology

Comparative Analysis of Human Hepatic Lesions in Dengue, Yellow Fever, and Chikungunya: Revisiting Histopathological Changes in the Light of Modern Knowledge of Cell Pathology

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