Illumination of Arabidopsis roots induces immediate burst of ROS production

Yokawa, Ken; Kagenishi, Tomoko; Kawano, Tomonori; Mancuso, Stefano; Baluška, Frantǐsek

doi:10.4161/psb.6.10.18165

Cited by 83 publications

(95 citation statements)

References 27 publications

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“…DAB staining has been often used to visualise the generation of H 2 O 2 in planta (Asai et al, 2010;Dubreuil-Maurizi et al, 2010;Kobayashi et al, 2012;L'Haridon et al, 2011;Liao et al, 2012;Rojas et al, 2012;Simon et al, 2013;Torres et al, 2005;Yokawa et al, 2011;Zhang et al, 2012). The Amplex Red hydrogen peroxide/peroxidase activity assay also allows quantifying H 2 O 2 concentrations and consists in a non-fluorescent molecule that is oxidised by H 2 O 2 and becomes fluorescent in presence of peroxidase.…”

Section: Detection Of Rosmentioning

confidence: 99%

Reactive oxygen species and plant resistance to fungal pathogens

et al. 2015

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Reactive oxygen species (ROS) have been studied for their role in plant development as well as in plant immunity. ROS were consistently observed to accumulate in the plant after the perception of pathogens and microbes and over the years, ROS were postulated to be an integral part of the defence response of the plant. In this article we will focus on recent findings about ROS involved in the interaction of plants with pathogenic fungi. We will describe the ways to detect ROS, their modes of action and their importance in relation to resistance to fungal pathogens. In addition we include some results from works focussing on the fungal interactor and from studies investigating roots during pathogen attack.

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Section: Detection Of Rosmentioning

confidence: 99%

Reactive oxygen species and plant resistance to fungal pathogens

et al. 2015

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“…the roots are exposed to light. This may cause different responses to their environment and induces changes in their biochemistry and development 26,27 . In order to reduce the amount of light on the root system, black plastic foil was used to cover the water surface as well as a lid made of black aluminum foil covered the sample chamber.…”

Section: Set Up the Microscopementioning

confidence: 99%

Light Sheet Fluorescence Microscopy of Plant Roots Growing on the Surface of a Gel

Wangenheim

Hauschild

Friml

2017

JoVE

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One of the key questions in understanding plant development is how single cells behave in a larger context of the tissue. Therefore, it requires the observation of the whole organ with a high spatial-as well as temporal resolution over prolonged periods of time, which may cause phototoxic effects. This protocol shows a plant sample preparation method for light-sheet microscopy, which is characterized by mounting the plant vertically on the surface of a gel. The plant is mounted in such a way that the roots are submerged in a liquid medium while the leaves remain in the air. In order to ensure photosynthetic activity of the plant, a custom-made lighting system illuminates the leaves. To keep the roots in darkness the water surface is covered with sheets of black plastic foil. This method allows long-term imaging of plant organ development in standardized conditions.

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“…2 Arabidopsis roots speed-up their growth significantly if illuminated. In addition, we also showed that Arabidopsis roots change their physiological aspects to perform the light avoidance over salt stress if kept under light conditions.…”

mentioning

confidence: 99%