Illuminating the detection chain of bacterial bioreporters

Meer, Jan Roelof van der; Tropel, David; Jaspers, Marco C. M.

doi:10.1111/j.1462-2920.2004.00655.x

Cited by 148 publications

(108 citation statements)

References 88 publications

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“…Compared with instrumental analysis, microbial biosensors show lower sensitivity [46], poor specificity [3], and delay responses due to the time required for reporter gene expression. Continuous researches to resolve such limitations of microbial biosensors have been investigated by exploiting more sensitive promoters [26], refining host strain [1], designing strains to produce modifying regulatory proteins and enzymes [12,27,30,37], surface expressed or periplasmic binding proteins [21,24], and controlling bacterial physiology [22].…”

Section: Configurationsmentioning

confidence: 99%

Applications of Microbial Whole-Cell Biosensors in Detection of Specific Environmental Pollutants

Shin¹

2011

Journal of Life Science

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Microbial whole-cell biosensors can be excellent analytical tools for monitoring environmental pollutants. They are constructed by fusing reporter genes (e.g., lux, gfp or lacZ) to inducible regulatory genes which are responsive to the relevant pollutants, such as aromatic hydrocarbons and heavy metals. A large spectrum of microbial biosensors has been developed using recombinant DNA technology and applied in fields as diverse as environmental monitoring, medicine, food processing, agriculture, and defense. Furthermore, their sensitivity and target range could be improved by modification of regulatory genes. Recently, microbial biosensor cells have been immobilized on chips, optic fibers, and other platforms of high-throughput cell arrays. This paper reviews recent advances and future trends of genetically modified microbial biosensors used for monitoring of specific environmental pollutants.

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Section: Configurationsmentioning

confidence: 99%

Applications of Microbial Whole-Cell Biosensors in Detection of Specific Environmental Pollutants

Shin¹

2011

Journal of Life Science

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“…The final goal is to fuel the use of the wholecell biosensors as a new paradigm in soil and agricultural sciences. Detailed description of the genetic construction whole-cell biosensor and signal detection systems are out of the scopes of the present review, and we refer to previous comprehensive reviews [8,39,42,66,71,83,84,130,131,134].…”

Section: Introductionmentioning

confidence: 99%

Light dazzles from the black box: whole-cell biosensors are ready to inform on fundamental soil biological processes

Renella

2016

Chem. Biol. Technol. Agric.

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Whole-cell biosensors are natural or engineered microorganisms producing signals in response to specific stimuli. This review introduces the use of whole-cell biosensors for the study of the soil system, discuss the recent developments and some current limitations and draws future prospects of the whole-cell biosensors for application to the study of the agro-ecosystems. The review focuses mainly on the lux-and gfp-inserted whole-cell biosensors producing bioluminescence and multicoloured fluorescent proteins, which allow an easy and reproducible detection of the signals from a large number of prokaryotic and eukaryotic soil-borne microorganisms. This review also points out how the whole-cell biosensors indicate the bioavailability of selected analyte, an information that cannot be straight forwardly extrapolated using the chemical methods of soil analysis. However, regardless of the immense progress in biotechnology and genetics that allows to construct whole-cell biosensors for virtually detecting any chemical at ultra low concentrations, the soil still remains the most extreme natural system to be studied with these biotechnological analytical tools. Although a lack of standardization for most of the constructed whole-cell biosensors along with the scarce knowledge of their performance concur to prevent their use in the official methods of soil and environmental analysis, owing to their stability and selectivity we restate that the whole-cell biosensors are ready to provide information on the main processes occurring in soil, and represent unprecedented sensitive tools for improving agriculture and for soil monitoring.

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“…The major attributes of a good biosensing system are its specificity, reliability, ability to function in optically opaque solution, real-time analysis and simplicity of operation (D'Souza, 2001). Bacterial biosensors are microorganisms engineered to detect target chemical compounds or changes in physico chemical conditions through inducible expression of reporter protein (Vandeer meer, 2004). Several metal specific bacterial sensors for the detection of bioavailable metals were developed with fusing metal regulatory protein gene with various reporter genes by many authors (Selifonova et al, 1993;Tauriainen et al, 1998;Ivask et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Construction of green fluorescent protein based bacterial biosensor for heavy metal remediation

Raja

Selvam

2011

Int. J. Environ. Sci. Technol.

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ABSTRACT:Environmental contamination by heavy metals is a worldwide problem. Therefore, it is necessary to develop sensitive, effective and inexpensive methods, which can efficiently monitor and determine the level of hazardous metals in the environment. Conventional techniques to analyze metals, suffer from the disadvantages of high cost. Alternatively, development of simple system for monitoring heavy metals pollution is therefore needed. The present approach is based on the use of bacteria that are genetically engineered so that a measurable signal is produced when the bacteria are in contact with the bioavailable metal ions. Reporter genes are widely used as genetic tools for quantification and detection of specific cell population, gene expression and constructing whole cell biosensors as specific and sensitive devices for measuring biologically relevant concentrations of pollutants. An attempt has been made to construct the reporter gene enhanced green fluorescent protein and was expressed under the control of cadR gene, responsible for cadmium resistance. Recombinant strain Escherichia coli cadR30 was used, that carried cadR gene in pET30b expression vector and cloned. Clones confirmed by the expression of enhanced green fluorescent protein was detected under ultraviolet illumination and separated on sodium dodecyl sulfate polyacrylamide gel electrophoresis. The construction of green fluorescent protein based Escherichia coli bacterial biosensor was developed based on green fluorescent protein expression under the control cadR gene of Pseudomonas aeruginosa BC15. The constructed bacterial biosensor is useful and applicable in determining the availability of heavy metals in soil and wastewater.

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Illuminating the detection chain of bacterial bioreporters

Cited by 148 publications

References 88 publications

Applications of Microbial Whole-Cell Biosensors in Detection of Specific Environmental Pollutants

Applications of Microbial Whole-Cell Biosensors in Detection of Specific Environmental Pollutants

Light dazzles from the black box: whole-cell biosensors are ready to inform on fundamental soil biological processes

Construction of green fluorescent protein based bacterial biosensor for heavy metal remediation

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