Construction of green fluorescent protein based bacterial biosensor for heavy metal remediation

Raja, Chellaiah Edward; Selvam, Govindan Sadasivam

doi:10.1007/bf03326262

Cited by 28 publications

(7 citation statements)

References 41 publications

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“…An Escherichia coli bacterial biosensor was developed based on green fluorescent protein expression under the control of the cadR gene of Pseudomonas aeruginosa BC15. The constructed bacterial biosensor proved to be useful in determining the availability of heavy metals in soil and wastewater [84]. A review article on the design and development of enzyme-, DNA-, immuno- and whole-cell-based biosensors for the detection of heavy metal toxicity is available [85]…”

Section: Measurement Of Bioavailable Metal Concentrationmentioning

confidence: 99%

Bioavailability of Heavy Metals in Soil: Impact on Microbial Biodegradation of Organic Compounds and Possible Improvement Strategies

Olaniran

Balgobind

Pillay

2013

IJMS

495

197

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Co-contamination of the environment with toxic chlorinated organic and heavy metal pollutants is one of the major problems facing industrialized nations today. Heavy metals may inhibit biodegradation of chlorinated organics by interacting with enzymes directly involved in biodegradation or those involved in general metabolism. Predictions of metal toxicity effects on organic pollutant biodegradation in co-contaminated soil and water environments is difficult since heavy metals may be present in a variety of chemical and physical forms. Recent advances in bioremediation of co-contaminated environments have focussed on the use of metal-resistant bacteria (cell and gene bioaugmentation), treatment amendments, clay minerals and chelating agents to reduce bioavailable heavy metal concentrations. Phytoremediation has also shown promise as an emerging alternative clean-up technology for co-contaminated environments. However, despite various investigations, in both aerobic and anaerobic systems, demonstrating that metal toxicity hampers the biodegradation of the organic component, a paucity of information exists in this area of research. Therefore, in this review, we discuss the problems associated with the degradation of chlorinated organics in co-contaminated environments, owing to metal toxicity and shed light on possible improvement strategies for effective bioremediation of sites co-contaminated with chlorinated organic compounds and heavy metals.

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Section: Measurement Of Bioavailable Metal Concentrationmentioning

confidence: 99%

Bioavailability of Heavy Metals in Soil: Impact on Microbial Biodegradation of Organic Compounds and Possible Improvement Strategies

Olaniran

Balgobind

Pillay

2013

IJMS

495

197

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“…However, the presence of a cadA gene (cadA provides resistance against Pb and Cd) could not be confirmed with the primers designed in this study probably due to the variations in sequences in the primer annealing region. The genes involved in metal resistance have been successfully used for the construction of biosensors, such as the reported use of copA in Cu biomonitoring (Raja and Selvam, 2011).…”

Section: Discussionmentioning

confidence: 99%

Characterization of a Multi-Metal Resistant Bacillus cereus Strain TWSL_5 and it’s Bioremediation Potential for Cr, Pb, Zn and Fe

Kumari¹,

Chandrasekharan²,

Wijayarathna³

2021

American Journal of Environmental Sciences

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An effluent sample collected from a textile dyeing factory was screened to isolate metal resistant endogenous bacteria using standard microbiology techniques. A bacterium with the highest metal resistance was characterized by 16S rRNA gene sequencing and the bacterial genome was screened for any possible metal resistant genes using the Polymerase Chain Reaction (PCR). The bioremoval ability of the isolate was evaluated using a multi-metal contaminated industrial effluent. Metal immobilisation was assayed using Scanning Electron Microscopy and Energy-Dispersive X-ray (SEM-EDX) analysis. An isolate (strain TWSL_5) was characterized as a Bacillus cereus strain. MICs of the strain TWSL_5 were found to be 75 mg/L (copper), 50 mg/L (cadmium) and 1000 mg/L (lead). The MIC for lead is among the highest reported for the species, B. cereus. The strain TWSL_5 showed a higher metal removal activity (p<0.001) for copper (93.13±0.17%) and lead (85±1.4%) in spiked cultures. The bacterium could successfully remove lead (100±0.00%), iron (68.22±0.07%), zinc (88.71±0.62%) and chromium (100±0.00%) from an effluent in 7 days of incubation at pH 6 and an insoluble black solid formed was detected as iron by SEM-EDX. The presence of copA in the genome of TWSL_5 was confirmed by PCR followed by sequence analysis. The presence of a gene involved in heavy metal resistance (copA), metal immobilization ability and the multi-metal removal activity of TWSL_5 in both spiked cultures and in an industrial effluent, indicates its potential to be used in metal bioremediation of industrial wastewater.

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“…In recent years, the utilization of recombinant microbes gains the popularity as the inserted genes can produce selected signals which are not available in naturally occurring cells [48][49][50]. The recombinant microbes can be used to enhance the sensitivity and specificity of the biosensors developed, however, as detailed review by Cases et al [51], the extensive usage of recombinant cells should proceed by considering the effect of the transgenic organisms to the environment.…”

Section: Issues and Limitations In The Development Of Whole Cell-base...mentioning

confidence: 99%

Whole Cell-based Biosensors for Environmental Heavy Metals Detection

Teo

Wong

Nilai³

2014

ARRB

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Biosensors have emerged as new alternatives in environmental toxicity assessment. In the development of biosensors for heavy metals detection in environment, whole cells are highly favored as these cells are able to reflect the real toxicity effects of heavy metals to living organisms. For heavy metals detection, the integration of several types of cells such as bacteria, cyanobacteria, and algae into biosensors development has been widely reported. The usage of other cells such as plant cell, protozoa, and yeast has been reported as well. Although these biosensors are highly sensitive to heavy metals, the detection is still limited to the heavy metals which are bioavailable to the cells. Besides, the response of whole cells to wide range of heavy metals makes them excellent tools for wide spectrum screening but lack of specificity in detection. Whole cells are living entities with complex biochemical processes, which make the optimization of whole cell-based biosensors a tedious process, while maintaining the stability and storability are still challenging tasks. Although naturally occurring cells are highly favored, some reports show that recombinant cells can be a choice with better performance. In this paper, the usage of whole cells in biosensors for heavy metals detection and some of the current issues which are tied to the development of these biosensors are reviewed.

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Construction of green fluorescent protein based bacterial biosensor for heavy metal remediation

Cited by 28 publications

References 41 publications

Bioavailability of Heavy Metals in Soil: Impact on Microbial Biodegradation of Organic Compounds and Possible Improvement Strategies

Bioavailability of Heavy Metals in Soil: Impact on Microbial Biodegradation of Organic Compounds and Possible Improvement Strategies

Characterization of a Multi-Metal Resistant Bacillus cereus Strain TWSL_5 and it’s Bioremediation Potential for Cr, Pb, Zn and Fe

Whole Cell-based Biosensors for Environmental Heavy Metals Detection

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