Illuminating life's building blocks

Fessenden, Marissa

doi:10.1038/533565a

Cited by 7 publications

(3 citation statements)

References 11 publications

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“…MP = 256 °C. 1 H NMR (400 MHz, Acetone-d6), δ (ppm): 7.40 and 7.70 (2H, NH 2 ), 6.69 and 7.55-8.62 (6H, CH Ar ), 8.84 (s, 1H, =CH-Ar), 9.60 (s, 1H, OH), 10.30 (s, 1H, NH). Selected IR bands (KBr pellet, cm -1 ): 3420 (OH), 3320a /3200s (NH 2 ), 3177 (NH), 3030 (C-H Ar ), 1577 (C=C), 1511 (C=N), 1115/ 783 (C=S), 967 (N-N).…”

Section: Synthesis Of (E)-2-((4-hydroxynaphthalen-1-yl)methylene)hydrmentioning

confidence: 99%

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New thiosemicarbazone Schiff base ligands: Synthesis, characterization, catecholase study and hemolytic activity

Nehar

Mahboub

Louhibi

et al. 2020

Journal of Molecular Structure

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Two novel thiosemicarbazones ligands have been synthesized and characterized by FT-IR, ESI-MS, 1 H NMR, and also by single-crystal X-ray diffraction for L1. The crystal structure shows that L1 molecules are planar and are connected via N-H-S and O-H-S interactions. The catecholase activity of is situ copper and cobalt complexes of this ligands has been investigated against 3,5-di-tert-butylcatechol. The progress of the oxidation reactions was closely monitored over time following the strong peak of 3,5-DTBC using UV-Vis. Oxidation rates were determined from the initial slope of absorbance vs. time plots, then analyzed by Michaelis-Menten equations. Catechol oxidation reactions were realized using different concentrations of copper and cobalt acetate and ligands (L/Cu: 1/1, 1/2, 2/1). The results show that all complexes were able to catalyze the oxidation of 3,5-DTBC. Acetate complexes have the highest activity. CuL1 and CoL1 complexes act as a catalyst and inhibitor. While copper and cobalt complexes obtained from ligand L2 illustrate concentration-independent oxidation activation. The hemolysis study performed by L1 increases as a function of its concentration. However, ligand L2 has no hemolytic effect.

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Section: Synthesis Of (E)-2-((4-hydroxynaphthalen-1-yl)methylene)hydrmentioning

confidence: 99%

“…Proteins are the most important building blocks of a living cell. They carry out a large variety of functions [1], such as: structural stabilization, molecule transport and catalysis. Metalloproteins are estimated to represent about one third of all proteins [2].…”

Section: Introductionmentioning

confidence: 99%

New thiosemicarbazone Schiff base ligands: Synthesis, characterization, catecholase study and hemolytic activity

Nehar

Mahboub

Louhibi

et al. 2020

Journal of Molecular Structure

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“…Semiconductor quantum dots (QDs) are an emerging class of fluorescent probes with outstanding optical properties (e.g., fluorescent intensity and photostability) compared with fluorescent dyes and fluorescent proteins. − However, the applications of QDs for imaging specific targets in the interior of live cells have been greatly limited by poor performance of intracellular targeted delivery of QDs. ,,− Intracellular targeted delivery of nanometer scale entities such as QDs is highly challenging due to the many formidable cellular transport barriers to overcome in order to reach the intracellular targets. ,,− These biotransport barriers primarily include the cell membrane, intracellular vesicle membrane (if the cellular entry mechanism is endocytosis), crowded cytoplasm, and nonspecific binding with proteins. ,,− We propose that greatly enhanced ability of overcoming the cellular transport barriers could be provided by a dramatic change to the surface design of QD probes, that is, using QD probes based on predominantly hydrophobic surface instead of the commonly used predominantly hydrophilic surface. In the present work, we investigate the hypothesis that bare hydrophobic QDs can directly penetrate through live cell membranes without disrupting the membrane integrity.…”

Section: Introductionmentioning

confidence: 99%

Direct and Noninvasive Penetration of Bare Hydrophobic Quantum Dots through Live Cell Membranes

Xie

Mei

Sun

et al. 2019

ACS Biomater. Sci. Eng.

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Semiconductor quantum dots (QDs) possess outstanding optical properties as fluorescent probes, but their applications in live cell intracellular imaging are hindered by various cellular transport barriers. Inspired by membrane proteins inserting their nanometer-scale hydrophobic surface into biomembranes, the present work aims to investigate the possibility that bare hydrophobic QDs could penetrate through live cell membranes without disrupting the membrane integrity. We utilize live cell spinning disk confocal microscopy to image and track the cellular transport process of bare hydrophobic QDs in the presence of a small percentage of three different organic cosolvents, namely, tetrahydrofuran (THF), chloroform, and hexane. A major finding is that, under certain cosolvent conditions, bare hydrophobic QDs can indeed penetrate through biomembranes in a noninvasive manner. Results of this work offer us guidance to design a new class of nanobioprobes based on combining hydrophobic nanoscale surface and cosolvent, and they provide key new pieces to the emerging complex and sophisticated picture of nanostructure–biosystem interactions.

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A potent, minimally invasive and simple strategy of enhancing intracellular targeted delivery of Tat peptide-conjugated quantum dots: organic solvent-based permeation enhancer

et al. 2018

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Illuminating life's building blocks

Cited by 7 publications

References 11 publications

New thiosemicarbazone Schiff base ligands: Synthesis, characterization, catecholase study and hemolytic activity

New thiosemicarbazone Schiff base ligands: Synthesis, characterization, catecholase study and hemolytic activity

Direct and Noninvasive Penetration of Bare Hydrophobic Quantum Dots through Live Cell Membranes

A potent, minimally invasive and simple strategy of enhancing intracellular targeted delivery of Tat peptide-conjugated quantum dots: organic solvent-based permeation enhancer

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