A potent, minimally invasive and simple strategy of enhancing intracellular targeted delivery of Tat peptide-conjugated quantum dots: organic solvent-based permeation enhancer

Yong, Xueqing; Yang, Xuan; Emory, Steven R.; Wang, Jun; Dai, Jie; Yu, Xiang; Mei, Ling; Xie, Jianping; Ruan, Gang

doi:10.1039/c8bm00928g

Cited by 14 publications

(11 citation statements)

References 48 publications

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“…Exosomes, 40–150 nm extracellular vesicles (EVs) released by cells, have emerged as a novel endogenous delivery system [18–21]. As natural carriers that transfer bioactive molecules between cells, exosomes present several features, including low immunogenicity, biodegradability, ability to encapsulate endogenous bioactive molecules, and the ability to cross the BBB [22–24]. In previous work, to improve the targeting characteristics of exosomes, we developed a rapid and efficient method to conjugate functional ligands onto exosomal surfaces using bio-orthogonal copper-free azide alkyne cyclo-addition (click chemistry) [25].…”

Section: Introductionmentioning

confidence: 99%

Exosome-mediated targeted delivery of miR-210 for angiogenic therapy after cerebral ischemia in mice

et al. 2019

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Background Accumulating evidence shows that microRNA-210 (miR-210) holds great promise to improve angiogenesis for brain tissue repair after cerebral ischemia. However, safe and efficient delivery of miR-210 via intravenous administration is still a challenge. In the past decade, exosomes have emerged as a novel endogenous delivery system. Here, c(RGDyK) peptide is conjugated to exosomes, and they are loaded with cholesterol-modified miR-210 (RGD-exo:miR-210). Results In a transient middle cerebral artery occlusion (MCAO) mouse model, the RGD-exo:miR-210 targets the lesion region of the ischemic brain after intravenous administration, resulting in an increase in miR-210 at the site. Furthermore, RGD-exo:miR-210 are administered once every other day for 14 days, and the expressions of integrin β 3 , vascular endothelial growth factor (VEGF) and CD34 are significantly upregulated. The animal survival rate is also enhanced. Conclusions These results suggest a strategy for the targeted delivery of miR-210 to ischemic brain and provide an angiogenic agent for the treatment of ischemic stroke. Electronic supplementary material The online version of this article (10.1186/s12951-019-0461-7) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Exosome-mediated targeted delivery of miR-210 for angiogenic therapy after cerebral ischemia in mice

et al. 2019

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“…In this study, the results demonstrated that smart nano delivery efficiently accumulated in tumour sites and induced apoptosis in treated cells [100]. Moreover, Yong et al investigated the use of permeation enhancers based on organic solvents [101]. They demonstrated that the addition of organic solvents, such as dimethyl sulfoxide, to the aqueous nanomaterial solution can enhance intracellular vesicle escape without compromising membrane integrity.…”

Section: Cell-penetrating Peptide Conjugation With Nanoparticles Fmentioning

confidence: 97%

“…They demonstrated that the addition of organic solvents, such as dimethyl sulfoxide, to the aqueous nanomaterial solution can enhance intracellular vesicle escape without compromising membrane integrity. Through conjugation of QD NP with CPP and organic solvent, the authors observed a significant enhancement in translocation across cell membranes [101].…”

Section: Cell-penetrating Peptide Conjugation With Nanoparticles Fmentioning

confidence: 99%

Combination of Cell-Penetrating Peptides with Nanoparticles for Therapeutic Application: A Review

2019

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Cell-penetrating peptides (CPPs), also known as protein translocation domains, membrane translocating sequences or Trojan peptides, are small molecules of 6 to 30 amino acid residues capable of penetrating biological barriers and cellular membranes. Furthermore, CPP have become an alternative strategy to overcome some of the current drug limitations and combat resistant strains since CPPs are capable of delivering different therapeutic molecules against a wide range of diseases. In this review, we address the recent conjugation of CPPs with nanoparticles, which constitutes a new class of delivery vectors with high pharmaceutical potential in a variety of diseases.

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“…After conjugation with CPPs, the cellular internalization of NPs can be enhanced significantly. In particular, the peptides containing special lysine (K)-, arginine (R)-, or proline (P)-rich motifs (known as nuclear localization sequences (NLSs)) can efficiently transport NPs into the nucleus [ 133 , 138 , 139 , 140 , 141 , 142 , 143 , 144 , 145 , 146 , 147 , 148 , 149 ]. As early as 1999, Josephson and colleagues found that Tat peptide modified SPIOs were internalized into lymphocytes over 100-fold more efficiently than nonmodified NPs [ 138 ].…”

Section: Employing Peptide Functionalized Nanoparticle As Positive Tumor-targeting Nanomedicinesmentioning

confidence: 99%

The Peptide Functionalized Inorganic Nanoparticles for Cancer-Related Bioanalytical and Biomedical Applications

Jian

Sun

et al. 2021

Molecules

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In order to improve their bioapplications, inorganic nanoparticles (NPs) are usually functionalized with specific biomolecules. Peptides with short amino acid sequences have attracted great attention in the NP functionalization since they are easy to be synthesized on a large scale by the automatic synthesizer and can integrate various functionalities including specific biorecognition and therapeutic function into one sequence. Conjugation of peptides with NPs can generate novel theranostic/drug delivery nanosystems with active tumor targeting ability and efficient nanosensing platforms for sensitive detection of various analytes, such as heavy metallic ions and biomarkers. Massive studies demonstrate that applications of the peptide–NP bioconjugates can help to achieve the precise diagnosis and therapy of diseases. In particular, the peptide–NP bioconjugates show tremendous potential for development of effective anti-tumor nanomedicines. This review provides an overview of the effects of properties of peptide functionalized NPs on precise diagnostics and therapy of cancers through summarizing the recent publications on the applications of peptide–NP bioconjugates for biomarkers (antigens and enzymes) and carcinogens (e.g., heavy metallic ions) detection, drug delivery, and imaging-guided therapy. The current challenges and future prospects of the subject are also discussed.

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A potent, minimally invasive and simple strategy of enhancing intracellular targeted delivery of Tat peptide-conjugated quantum dots: organic solvent-based permeation enhancer

Abstract: Organic solvent in low percentage offers a great way to enhance vesicle escape and intracellular targeted delivery of nanomaterials.

Cited by 14 publications

References 48 publications

Exosome-mediated targeted delivery of miR-210 for angiogenic therapy after cerebral ischemia in mice

Exosome-mediated targeted delivery of miR-210 for angiogenic therapy after cerebral ischemia in mice

Combination of Cell-Penetrating Peptides with Nanoparticles for Therapeutic Application: A Review

The Peptide Functionalized Inorganic Nanoparticles for Cancer-Related Bioanalytical and Biomedical Applications

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