Illumina MiSeq sequencing reveals the key microorganisms involved in partial nitritation followed by simultaneous sludge fermentation, denitrification and anammox process

Wang, Bo; Peng, Yongzhen; Guo, Yuanyuan; Zhao, Mingming; Wang, Shuying

doi:10.1016/j.biortech.2016.01.072

Cited by 131 publications

(22 citation statements)

References 35 publications

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“…Similar results were also reported in aerobic sludge samples collected from tannery WWTPs, and extremely high abundance was detected (4.64%-15.81%) [26]. In addition, Thauera bacteria were frequently detected in other wastewater treatment bioreactors, and they were considered to be functionally important populations [15,32]. In a recent study, the genus Thauera was identified as the most abundant microorganism in a granular sludge system, and it was proved to harbor perfect nitrification ability, with a 99.8% NH 4 + -N (50 mg/l) removal efficiency in 48 h [6].…”

Section: Identification Of the Core Generasupporting

confidence: 84%

“…In this study, relatively high Shannon indexes were obtained for all samples expect archaeal community in sample AS-B, corresponding to high OTU values. The diversity and richness of the tannery wastewater were similar to those of municipal wastewater plants and significantly higher than those of other industrial wastewaters [15,18,25]. Ma et al [25] investigated the bacterial community in coking wastewater, and the Shannon indexes were in the range of 3.0-4.0, corresponding to low OTU values ranging from 365 to 776.…”

Section: Overall Analysis Of Illumina Miseq Sequencingmentioning

confidence: 96%

“…In a recent study, the genus Thauera was identified as the most abundant microorganism in a granular sludge system, and it was proved to harbor perfect nitrification ability, with a 99.8% NH 4 + -N (50 mg/l) removal efficiency in 48 h [6]. With the exception of nitrification ability, Thauera was also known to be capable of denitrification and organic compound biodegradation [15,26]. Mao et al [35] isolated three Thauera strains from a coking wastewater treatment system that could degrade The color intensity in each panel indicates the relative abundance of a genus in each sample, and the abundance was defined as the number of sequences assigned to that taxon divided by the total number of sequences per sample.…”

Section: Identification Of the Core Generamentioning

confidence: 99%

“…Recently, high-throughput sequencing technologies such as 454 or Illumina sequencing have been developed to comprehensively identify the microbial diversity in different samples, including activated sludge, soil, ocean water, and food [14][15][16][17]. In addition, it was found that the municipal activated sludge from different plants shared some dominant and core genera, such as Zoogloea and Prosthecobacter [18].…”

Section: Introductionmentioning

confidence: 99%

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Microbial Community of Tannery Wastewater Involved in Nitrification Revealed by Illumina MiSeq Sequencing

Ma¹,

Wu²,

Huang³

et al. 2018

Journal of Microbiology and Biotechnology

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The aim of this study was to investigate the microbial community of three tannery wastewater treatment plants (WWTPs) involved in nitrification by Illumina MiSeq sequencing. The results showed that highly diverse communities were present in tannery wastewater. A total of six phyla, including Proteobacteria (37-41%), Bacteroidetes (6.04-16.80), Planctomycetes (3.65-16.55), Chloroflexi (2.51-11.48), Actinobacteria (1.91-9.21), and Acidobacteria (3.04-6.20), were identified as the main phyla, and Proteobacteria dominated in all the samples. Within Proteobacteria, Beta-proteobacteria was the most abundant class, with the sequence percentages ranging from 9.66% to 17.44%. Analysis of the community at the genus level suggested that Thauera, Gp4, Ignavibacterium, Phycisphaera, and Arenimonas were the core genera shared by at least two tannery WWTPs. A detailed analysis of the abundance of ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB) indicated that Nitrosospira, Nitrosomonas, and Nitrospira were the main AOB and NOB in tannery wastewater, respectively, which exhibited relatively high abundance in all samples. In addition, real-time quantitative PCR was conducted to validate the results by quantifying the abundance of the AOB and total bacteria, and similar results were obtained. Overall, the results presented in this study may provide new insights into our understanding of key microorganisms and the entire community of tannery wastewater and contribute to improving the nitrogen removal efficiency. S 1170 Ma et al. J. Microbiol. Biotechnol.

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Section: Identification Of the Core Generasupporting

confidence: 84%

Section: Overall Analysis Of Illumina Miseq Sequencingmentioning

confidence: 96%

Section: Identification Of the Core Generamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Microbial Community of Tannery Wastewater Involved in Nitrification Revealed by Illumina MiSeq Sequencing

Ma¹,

Wu²,

Huang³

et al. 2018

Journal of Microbiology and Biotechnology

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show abstract

“…The microbial community structure of a denitrifying reactor can change with different influent composition, process configuration and operating parameters . Thus assessing of microbial community composition and diversity in a biological wastewater treatment process is very useful in explaining the complex microbial interactions and substrate utilization . Nitrate can be reduced to nitrogen gas with four steps of enzymatic reaction, each step catalyzed by a specific reductase.…”

Section: Introductionmentioning

confidence: 99%

Impact of hydraulic retention time and current on the microbial community and denitrification genes in a continuous‐flow biofilm electrode reactor

Zhai

Pavlostathis

et al. 2018

J of Chemical Tech & Biotech

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BACKGROUND: A biofilm-electrode reactor (BER) was developed on the basis of hydrogenotrophic denitrification. The BER can be strengthened by adding organic matter, and the nitrate removal will be enhanced by a combination of heterotrophic and autotrophic denitrification. Denitrification in a biological system can be affected by operating parameters, and the microbial community and functional genes can be changed at the same time. In this study, the effect of hydraulic retention time (HRT) and current on the denitrification of a low chemical oxygen demand to nitrogen ratio (COD/N), saline wastewater in a continuous-flow BER was evaluated and compared to a conventional biofilm reactor (BR). RESULTS:The highest extent of nitrate removal was obtained at the optimum HRT (8 h) and current (10 mA). Low levels of ammonia (0.5-4 mg L −1 ), produced through dissimilatory nitrate reduction to ammonia (DNRA), were observed. Quantitative real-time polymerase chain reaction analysis revealed that the proportion of nirS-type denitrifiers (3.75-8.30%) exceeded nirK-type denitrifiers (0.24-0.90%) in both BR and BER. The genera of T78, Paracoccus and Azoarcus were identified as the dominant denitrifying bacteria in the BER. The cooperative activity of nitrate, nitrite and nitrous oxide reductase is necessary to reduce nitrate to dinitrogen in the denitrification process. The highest ratio of nosZ/nir genes in the bacterial community was 0.25 when the applied current was 10 mA. CONCLUSION: Compared to other denitrification reactors, the BER is more effective to treat low-COD/N, saline wastewater with short HRT and low current. RESULTS AND DISCUSSION Effect of HRT on denitrification performanceThe denitrification performance of BR and BER was investigated at five HRTs, decreasing from 16 to 12, 8, 4 and 2 h. The variation J Chem Technol Biotechnol 2019; 94: 933-941

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Recent Advances in the Biochemistry and Molecular Biology of Ammonia-Oxidizing Bacteria

Jha

Dutta

Dhawan

et al. 2023

Anammox Technology in Industrial Wastewater Treatment

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Illumina MiSeq sequencing reveals the key microorganisms involved in partial nitritation followed by simultaneous sludge fermentation, denitrification and anammox process

Cited by 131 publications

References 35 publications

Microbial Community of Tannery Wastewater Involved in Nitrification Revealed by Illumina MiSeq Sequencing

Microbial Community of Tannery Wastewater Involved in Nitrification Revealed by Illumina MiSeq Sequencing

Impact of hydraulic retention time and current on the microbial community and denitrification genes in a continuous‐flow biofilm electrode reactor

Recent Advances in the Biochemistry and Molecular Biology of Ammonia-Oxidizing Bacteria

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