Illumina-based sequencing analysis of pathogenic microorganisms in dental caries patients of different Chinese ethnic groups

Chen, Yun; Zhi-yan, LI; Chong, Zhao Zhong; Liu, Jing; Zhang, Xin; Zhu, Danshi

doi:10.1177/0300060519866939

Cited by 2 publications

(1 citation statement)

References 19 publications

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“…This demonstrated the cohorts we selected were comparative and that the samples we collected were typical ( Thompson and Pikis, 2012 ; Qudeimat et al, 2021 ). Leptotrichia was one of the predominant genera in the plaque from the WSLs niche, which was consistent with the previous report ( Yun et al, 2019 ). Leptotrichia could actively metabolize carbohydrates with higher membrane transport capacity ( Eribe and Olsen, 2017 ; Chen Y. et al, 2021 ).…”

Section: Discussionsupporting

confidence: 93%

Candida albicans enriched in orthodontic derived white spot lesions and shaped focal supragingival bacteriome

Yang

Xie

et al. 2023

Front. Microbiol.

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White spot lesions (WSLs) are common enamel infectious diseases in fixed orthodontic treatment, which might attribute to the dysbiosis of oral microbiome. However, the correlation of Candida albicans with oral bacteriome in WSLs still remains unrevealed. This study investigated the carriage of C. albicans and how it shaped the bacterial community in disease or healthy supragingival plaque, to explore the potential role of interkingdom interaction in orthodontic WSLs. In this study, 31 patients with WSLs (WSLs) and 23 healthy patients (Health) undergoing fixed orthodontic treatment were enrolled. The supragingival microbiota in both groups were determined using 16S rRNA gene sequencing. Colonization and abundance of C. albicans in the plaque were determined via culture-dependent and -independent methods. Among WSLs patients, the correlation of C. albicans and bacteriome was analyzed under QIIME2-based bioinformatics and Spearman’s correlation coefficient. The raw reads were deposited into the NCBI Sequence Read Archive (SRA) database (Accession Number: SRP404186). Significant differences in microbial diversity as well as composition were observed between WSLs and Health groups. Leptotrichia remarkably enriched in the WSLs group, while Neisseria and Cardiobacterium significantly enriched in the Health group. In addition, 45% of WSLs patients were C. albicans carriers but none in patients without WSLs. Among all WSLs patients, beta diversity and microbial composition were distinguished between C. albicans carriers and non-carriers. In C. albicans carriers, Corynebacterium matruchotii and Streptococcus mutans significantly enriched whereas Saccharibacteria_TM7_G-1 significantly depleted. The abundance of C. albicans was positively associated with bacteria such as Streptococcus mutans, while the negative correlation was detected between C. albicans and several bacteria such as Cardiobacterium hominis and Streptococcus sanguinis. Our study elucidated the distinguished supragingival plaque microbiome between orthodontic patients with and without WSLs. C. albicans frequently existed and enriched in orthodontic derived WSLs. The carriage of C. albicans shape plaque bacterial community in demineralized lesions and might play roles in WSLs pathogenesis.

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Section: Discussionsupporting

confidence: 93%

Candida albicans enriched in orthodontic derived white spot lesions and shaped focal supragingival bacteriome

Yang

Xie

et al. 2023

Front. Microbiol.

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Meta-Analysis Using NGS Data: The Veillonella Species in Dental Caries

Damé‐Teixeira

Lima

et al. 2021

Front. Oral. Health

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Objectives: In light of recent technological advances in Next-generation sequencing (NGS) and the accumulation of large, publicly available oral microbiome datasets, the need for meta-analysing data on caries microbiome is becoming feasible and essential. A consensus on the identification of enriched organisms in cariogenic dysbiotic biofilms would be reached. For example, members of the Veillonella genus have been detected in caries biofilms, and may have an underestimated contribution to the dysbiotic process. Hence, we aimed to determine the abundance of Veillonella species in dental caries in studies using NGS data.Materials and Methods: Analysis was performed according to the Preferred Reporting Items for Systematic Review and Meta-Analysis (registered at PROSPERO: CRD42020204150). Studies investigating microbial composition in saliva, dental biofilm, or carious dentin were included. Six databases and grey literature were searched. Two independent reviewers selected the papers and assessed the methodological quality.Results: Searches retrieved 1,323 titles, from which 38 studies were included in a qualitative synthesis, comprising a total of 1,374 caries and 745 caries-free individuals. Most studies analysed 16S rRNA amplicons, and only 5 studies used shotgun metagenomics and metatranscriptomics. A geographical bias was observed. The methodological quality was downrated in 81.5% of the studies due to the lack of criteria for defining cases and standard criteria used for measurement of the condition in a reliable way. Six studies on early childhood caries (ECC) were meta-analysed, confirming a significant enrichment of Veillonella spp. in caries-associated biofilms (but not saliva) when compared to caries-free controls [mean difference: 2.22 (0.54–3.90); p = 0.01].Conclusions:Veillonella spp. is more abundant in individuals suffering with ECC when compared to caries-free controls (very low evidence certainty), and should be considered for further studies to observe their metabolism in dental caries. There is an urgent need for a consensus in methodologies used to allow for more rigorous comparison between NGS studies, particularly including clinical data and details of caries diagnosis, as they are currently scarce. Inconsistent reporting on the NGS data affected the cross-study comparison and the biological connexions of the relative abundances on caries microbiome.

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Illumina-based sequencing analysis of pathogenic microorganisms in dental caries patients of different Chinese ethnic groups

Cited by 2 publications

References 19 publications

Candida albicans enriched in orthodontic derived white spot lesions and shaped focal supragingival bacteriome

Candida albicans enriched in orthodontic derived white spot lesions and shaped focal supragingival bacteriome

Meta-Analysis Using NGS Data: The Veillonella Species in Dental Caries

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