Illumina-based 16S metagenomic analysis of the indigenous gut microbiota of cavity-nesting bee <i>Megachile centuncularis</i>: a comparison with the cavity-nesting wasp <i>Ancistrocerus antilope</i>

Skrodenytė-Arbačiauskienė, Vesta; Budrienė, Anna; Blažytė‐Čereškienė, Laima; Budrys, Eduardas

doi:10.1080/00218839.2019.1614734

Cited by 3 publications

(6 citation statements)

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“…Several previous studies tested whether different insect species (mostly within the same genus or trophic niche) associate with different microbial communities. Differences between insect species have been found in aphids (Zepeda-Paulo et al, 2018;Fakhour et al, 2018;McLean et al, 2019;Gallo-Franco et al, 2019), mealybugs (Lin et al, 2019), reduviids (Rodríguez-Ruano et al, 2018), fruit flies (Ventura et al, 2018;Yong et al, 2017), beetles (Hulcr et al, 2012;Kolasa et al, 2019), silkworms (Chen et al, 2018), lepidopera (van Schooten et al, 2018;Liu et al, 2020), mosquitoes (Mancini et al, 2018), bees and wasps (Skrodenytė-Arbačiauskienė et al, 2019;Suenami et al, 2019). Conversely, other studies did not report differences when comparing different insect species (Berasategui et al, 2016;Ramalho et al, 2017;Meng et al, 2019a).…”

Section: Factors Influencing Insect Microbial Communitiesmentioning

confidence: 99%

Host species identity shapes the diversity and structure of insect microbiota

Malacrinò

2021

Preprint

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Microorganisms have an enormous impact on most of the life that inhabits our planet. Insects are an excellent example, as research showed that several microbial species are essential for insect nutrition, reproduction, fitness, defence and many other functions. More recently, we assisted to an exponential growth of studies describing the taxonomical composition of bacterial communities across insects' phylogeny. However, there is still an outstanding question that needs to be answered: which factors contribute most in shaping insects' microbiomes? This study tries to find an answer to this question by taking advantage of publicly available sequencing data and reanalysing over 4,000 samples of insect-associated bacterial communities under a common framework. Results suggest that insect taxonomy has a wider impact on the structure and diversity of their associated microbial communities than the other factors considered (diet, sex, life stage, sample origin and treatment). Also, a survey of the literature highlights several methodological limitations that needs to be considered in future research endeavours. This study proofs the amount of collective effort that lead to the current understanding of insect-microbiota interactions and their influence on insect biology, ecology and evolution with potential impact on insect conservation and management practices.

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Section: Factors Influencing Insect Microbial Communitiesmentioning

confidence: 99%

Host species identity shapes the diversity and structure of insect microbiota

Malacrinò

2021

Preprint

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“…The microbiota changes might cause by the living environment or pathogen infections (Kaltenpoth & Engl, 2014). Indeed, the significant decreased in microbiota richness has been reported from the mosquitos infected with entomopathogenic fungus, Beauveria bassiana, and also found in two hymenoptera insects, Megachile centuncularis and Ancistrocerus antelope, which have different disparate trophic specialization (Skrodenytė-Arbačiauskienė et al, 2019;Wei et al, 2017). In the current case, there is no obvious mycosis symptom was found in the P. lanyuhensis; therefore, the fungal infection and caused the abnormal physiological circumstance of P. lanyuhensis could be temporally excluded.…”

Section: Species Richness Of Bacteria From P Lanyuhensismentioning

confidence: 99%

“…Around this decade, the 16S metagenomic sequencing has been widely applied on insects for better understanding on the microbiota diversity in their intestines (Lim & Ab Majid, 2020; Sevim et al, 2010; Shelomi et al, 2013; Skrodenytė‐Arbačiauskienė et al, 2019; Villegas & Pimenta, 2014). The microbiota of several insect species have been described to unravel the bacterial communities of the intestinal tract in insects, including Dipteran ( Zeugodacus cucurbitae, Bactrocera dorsalis, Anopheles stephensi , and Aedes albopictus etc.…”

Section: Introductionmentioning

confidence: 99%

“…; Chen et al, 2018; Sevim et al, 2010), and Hymenopteran ( Megachile centuncularis, Megaphragma amalphitanum , and different honey bee [ Apis mellifera ] subspecies etc. ; Jeyaprakash et al, 2003; Skrodenytė‐Arbačiauskienė et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

“…Studies of insect gut microbiota have shown that gut microbiota may vary between insect species, their life stages (Hadapad et al, 2019; Villegas & Pimenta, 2014) or differences in feeding habit, especially for phytophagous insects (Chen et al, 2018; Lim & Ab Majid, 2020; Shelomi et al, 2013). Many studies have indicated that the insect gut microorganisms plays an important role in the development, digestion, metabolism, reproduction, immunity as well as many other physiological functions of their hosts (Chen et al, 2018; Hadapad et al, 2019; Pérez‐Ruiz et al, 2015; Skrodenytė‐Arbačiauskienė et al, 2019). The fact that exogenous bacteria may affect the lifespan of fruit fly ( Drosophila melanogaster ) further implicated the importance of gut microbiota in environmental fitness of the insect host (Brummel et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

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Comparison of gut microbiota of healthy and diseased walking sticks, Phasmotaenia lanyuhensis

Huang

Chen

et al. 2020

Arch Insect Biochem Physiol

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Research on gut microbiota of phytophagous insects has shown to be important for the physiological functions of insect hosts; however, little is known about the changes in gut microbiota when they are suffering from environmental stress or pathogen infections. During rearing of Phasmotaenia lanyuhensis (Phasmatodea: Phasmatidae), sluggish locomotion was usually followed by the death of the insect with a symptom of melanization in the front part of the abdomen. Therefore, the abnormal individuals were initially classified into moribund, light-and serioussymptom based on the level of abnormal physiological circumstances and melanization. The gut microbiota of these samples were further investigated by 16S metagenomic sequencing and the differences in bacterial abundance and structure of bacterial community were analyzed. A decrease in microbiota diversity was observed in the diseased P. lanyuhensis, with the abundance of phyla Proteobacteria and Firmicute relatively higher compared to those without symptom. Interestingly, principal component analysis based on the bacterial richness was correlated to the level of melanization symptom in the diseased P. lanyuhensis, suggested the change in bacterial microbiota

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Host species identity shapes the diversity and structure of insect microbiota

Malacrinò

2021

Molecular Ecology

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Illumina-based 16S metagenomic analysis of the indigenous gut microbiota of cavity-nesting bee Megachile centuncularis: a comparison with the cavity-nesting wasp Ancistrocerus antilope

Cited by 3 publications

References 12 publications

Host species identity shapes the diversity and structure of insect microbiota

Host species identity shapes the diversity and structure of insect microbiota

Comparison of gut microbiota of healthy and diseased walking sticks, Phasmotaenia lanyuhensis

Host species identity shapes the diversity and structure of insect microbiota

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