Host species identity shapes the diversity and structure of insect microbiota

Malacrinò, Antonino

doi:10.1111/mec.16285

Cited by 26 publications

(25 citation statements)

References 153 publications

(171 reference statements)

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“…Thus, our results support the idea that the soil microbiota shapes plant and herbivorous microbial communities via plants. Indeed, different soil microbiota can influence plant metabolism or physiology [33,34], and this can drive changes in leaf metabolite or physical composition [35], ultimately altering the diet of M. sexta and leading to changes in herbivore-associated microbiota [11,36]. Interestingly, our results showed a higher number of ASVs shared between herbivores and plant roots compared to the number of ASVs shared between herbivores and leaves and herbivores and rhizosphere.…”

Section: Discussionmentioning

confidence: 87%

Soil microbiota and herbivory drive the assembly of plant-associated microbial communities through different mechanisms

Malacrinò

Bennett

2022

Preprint

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Plant-associated microbiomes are key to shaping many aspects of plant biology, including their fitness, ecology, and evolution. While several studies focused on testing the effect of different factors (e.g., plant genotype, soil, stressors) on the assembly of plant microbiomes, we still know a little about the possible mechanisms that drive these effects. In this study we tested whether soil microbial community and herbivory influence the microbial community of tomato plants, and whether their influence in different plant compartments is driven by active (via plant) or passive (via microbial spillover) mechanisms. We grew our plants in soils hosting three different microbial communities, we covered (or not) the soil surface to prevent (or allow) passive microbial spillover between compartments, and we exposed them (or not) to herbivory by Manduca sexta. Results suggest that the soil-driven effect was detectable regardless of soil coverage, suggesting that this effect might be mediated by the plant. On the other hand, the herbivore-driven effect on the rhizosphere microbiota was detectable only when the soil cover was absent, suggesting that this effect might be driven by microbial spillover. These results promote understanding of the drivers of plant microbiome assembly, their relative strength, and the mechanisms behind them.

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Section: Discussionmentioning

confidence: 87%

Soil microbiota and herbivory drive the assembly of plant-associated microbial communities through different mechanisms

Malacrinò

Bennett

2022

Preprint

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“…One possible explanation for these differences is that host specificity may be more important than diet in shaping the fecal bacterial communities of these dragonflies. Host specificity refers to the degree to which a particular microbial community has adapted to the gut environment of the host, and the findings suggest that the closely related species O. lineostigma and O. melania may have developed unique microbial communities over time due to differences in their gut environment, despite their similar diets (Gupta & Nair 2020; Malacrinò 2022). As well, P. zonata have high microbial communities.…”

Section: Discussionmentioning

confidence: 99%

Gut microbiome of three species of Odonata

Lim

Park

et al. 2023

Entomological Research

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The influence of diet and host specificity on the fecal microbiome of three adult dragonfly species, Pseudothemis zonata, Orthetrum lineostigma, and Orthetrum melania, was investigated. The fecal bacterial communities were analyzed using 16S rRNA gene sequencing, and stable isotope analysis was used to investigate their food sources. The results showed significant differences in the composition of fecal bacterial communities among the three species, with host specificity potentially playing a more important role than diet. The dominant phyla in the fecal bacterial communities of all three species were Firmicutes, Proteobacteria, and Bacteroidetes. The operational taxonomic units (OTUs), Shannon index, and phylogenetic diversity index were not significantly different among the three species, indicating that there were no major differences in the diversity of the fecal bacterial communities. The stable isotope analysis showed that the food sources were similar among the three species, being primarily small insects found near the aquatic habitats. However, the fecal bacterial communities of two closely related species, O. lineostigma and O. melania, were different despite their similar food sources. In contrast, the fecal bacterial communities of O. lineostigma and P. zonata were similar, despite the different food sources of these two species. Our findings suggest that host specificity and diet can influence the composition of the intestinal microbiome in these insects, but the degree of influence may depend on the specific host and environmental conditions.

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“…The microbiomes of generalists and specialist herbivores could also vary simply due to differences in the contribution of heritable versus environmentally acquired microbes, the latter being more variable and transient. However, diet may in fact generally be a poor predictor of insect bacterial community composition 22 , 58 , with exceptions only in certain groups of herbivores 59 . For example, Lepidopteran larva have variable gut microbiomes that are likely shaped by the host plant they feed on, but general patterns associated with diet breadth are not observed due to the high turnover rate of these microbial communities 14 .…”

Section: Discussionmentioning

confidence: 99%

Aphid species specializing on milkweed harbor taxonomically similar bacterial communities that differ in richness and relative abundance of core symbionts

Enders

Hansen

Brichler

et al. 2022

Sci Rep

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Host plant range is arguably one of the most important factors shaping microbial communities associated with insect herbivores. However, it is unclear whether host plant specialization limits microbial community diversity or to what extent herbivores sharing a common host plant evolve similar microbiomes. To investigate whether variation in host plant range influences the assembly of core herbivore symbiont populations we compared bacterial diversity across three milkweed aphid species (Aphisnerii, Aphis asclepiadis, Myzocallis asclepiadis) feeding on a common host plant (Asclepias syriaca) using 16S rRNA metabarcoding. Overall, although there was significant overlap in taxa detected across all three aphid species (i.e. similar composition), some structural differences were identified within communities. Each aphid species harbored bacterial communities that varied in terms of richness and relative abundance of key symbionts. However, bacterial community diversity did not vary with degree of aphid host plant specialization. Interestingly, the narrow specialist A.asclepiadis harbored significantly higher relative abundances of the facultative symbiont Arsenophonus compared to the other two aphid species. Although many low abundance microbes were shared across all milkweed aphids, key differences in symbiotic partnerships were observed that could influence host physiology or additional ecological variation in traits that are microbially-mediated. Overall, this study suggests overlap in host plant range can select for taxonomically similar microbiomes across herbivore species, but variation in core aphid symbionts within these communities may still occur.

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Host species identity shapes the diversity and structure of insect microbiota

Cited by 26 publications

References 153 publications

Soil microbiota and herbivory drive the assembly of plant-associated microbial communities through different mechanisms

Soil microbiota and herbivory drive the assembly of plant-associated microbial communities through different mechanisms

Gut microbiome of three species of Odonata

Aphid species specializing on milkweed harbor taxonomically similar bacterial communities that differ in richness and relative abundance of core symbionts

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