IL1B triggers inflammatory cytokine production in bovine oviduct epithelial cells and induces neutrophil accumulation via CCL2

Abstract

Problem The oviduct is essential for reproduction. We previously showed that oviduct epithelial cells (OECs) isolated from aged cows expressed higher levels of inflammatory cytokines, including interleukin (IL) 1A and IL1B. In addition, aging is associated with tissue dysfunction and cellular senescence via a senescence‐associated secretory phenotype (SASP) and immune cell accumulation. We investigated whether IL1A or IL1B causes SASP production, cellular senescence, and inflammatory responses in bovine OECs. Method of Study The OECs were isolated from bovine oviducts from young (mean 50.3 months) and aged cows (mean 157.0 months) and cultured. Results Treatment with IL1A or IL1B induced SASP production (IL8, IL6, TNFA, and CCL2) and mRNA expression of cell adhesion molecules in bovine OECs, but both IL1s did not induce cellular senescence in OECs and migration of polymorphonuclear neutrophils (PMNs). Cultured medium of OECs treated with IL1s, especially IL1B, dramatically induced PMN migration. Treatment with the CCL2 inhibitor, but not IL8 or its receptor CXCR2 inhibitors, significantly reduced immune cell migration in IL1B‐treated OEC‐cultured medium. Treatment with IL1B increased PMN adhesion to OECs, resulting in further SASP production in OECs due to a PMN‐OEC interaction. Conclusion We suggest that senescence‐associated IL1s cause SASP production in bovine OECs and CCL2 induced by IL1B is essential for the migration of immune cells to OECs. Specifically, IL1B regulates PMN migration and adhesion to bovine OECs, and PMNs accelerate inflammatory cytokine production from bovine OECs via a direct interaction. These phenomena may contribute to chronic oviductal inflammation, resulting in subfertility.

Comprehensive Analysis of Myeloid Signature Genes in Head and Neck Squamous Cell Carcinoma to Predict the Prognosis and Immune Infiltration

“…Some of the myeloid genes in our model have been shown to regulate multiple immune cells by previous studies. IL1B, belonging to the IL1 system, can drive senescence-associated secretory phenotypes (SASPs), which may affect immune cell activation and induce neutrophil accumulation ( 76 , 77 ). In addition, the shed ectodomain of LYVE1 expressed on M2 cells inhibited cancer cell proliferation ( 78 ).…”

Inhibition of ACSS2-mediated histone crotonylation alleviates kidney fibrosis via IL-1β-dependent macrophage activation and tubular cell senescence

“…Other than mediating macrophage polarization, considerable studies report that IL-1β may be involved in the senescence of several types of cells, including vascular smooth muscle cells 23 , astrocytes 24 , bovine oviduct epithelial cells 25 , mature chondrocytes 26 , etc. Recently, several studies have shown positive correlations between senescent cell accumulation and fibrosis in kidneys during ageing 27 – 29 and disease 30 – 32 .…”

Refining Protein-Level MicroRNA Target Interactions in Disease from Prediction Databases Using Sentence-BERT

“…But here, we also have a question whether only cancer-related genes have such a pattern. So we additionally selected 10 genes related to immune and inflammatory pathways, namely TNF, IL6, TGFB1, IL1B, IL10, IL17A, MMP9, CRP, TLR4, NLRP3 [29][30][31][32][33][34][35][36][37][38]. 342 miRNA gene pairwise scores were found using the Connection Building Method.…”