IL-8 gene polymorphism in acute biliary and non biliary pancreatitis: probable cause of high level parameters?

Abstract

Backgrounds/AimsInflammatory mediators of the innate immune response play fundamental roles in the pathogenesis of acute pancreatitis. The correlation between interleukin-8 (IL-8) gene polymorphism with types of acute pancreatitis and severity of pancreatitis, was evaluated in this study.MethodsAccording to the diagnostic criteria, 176 patients with acute pancreatitis were grouped into biliary (n=83) and nonbiliary pancreatitis (n=93). Healthy blood donors (n=100) served as controls. Serum alanine transaminase, aspartate transaminase, total and direct bilirubin, amylase, lypase, white blood cell count and c-reactive protein levels were evaluated to correlate with IL-8 rs4073 (-251T/A) polymorphism, which was analyzed using a real-time polymerase chain reaction method with melting point analysis.ResultsThe IL-8 AA genotype was detected with a significantly higher frequency among the patients with acute biliary pancreatitis having higher alanine transaminase levels than the median range. Homozygote alleles were significantly higher among patients with acute biliary pancreatitis having amylase levels higher than the median range.ConclusionsDetermination of the frequency of IL-8 polymorphism in acute pancreatitis is informative and provides further evidence concerning the role of IL-8 in laboratory tests.

Weak association between the interleukin-8 rs4073 polymorphism and acute pancreatitis: a cumulative meta-analysis

“…In the initial screening process, a total of 178 articles were retrieved based on our selection strategy from the databases. After the removal of duplicated literatures and articles that did not meet the inclusion criteria, 10 studies involving 1646 AP patients and 1816 controls were finally included in this meta-analysis [19–28]. Of these selected studies, six studies were published in English [19–21, 24–26], three were published in Chinese [22, 27, 28], the other one was published in Russian [23].…”

“…After the removal of duplicated literatures and articles that did not meet the inclusion criteria, 10 studies involving 1646 AP patients and 1816 controls were finally included in this meta-analysis [19–28]. Of these selected studies, six studies were published in English [19–21, 24–26], three were published in Chinese [22, 27, 28], the other one was published in Russian [23]. There were six studies conducted in China [20, 22, 24, 26–28], and one study conducted in U.S. [21], Russia [23], Turkey [19] and Hungary [25], respectively.…”

Genetic determination of pancreatitis

“…In one of the studies, heterozygous variants of IL-8 A/T mutation were more frequent in patients with severe AP, whereas a normal TT genotype was more often observed in patients with mild AP [29]. In turn, studies of IL-8 251A/T gene polymorphism in Chinese and Turkish populations did not show any significant correlations with the severity of the course of AP [30,31]. The researchers concluded that IL-8-251 gene polymorphism may be related with ethnic differences, which exert an effect on the results of studies [31].…”

“…The studies focused on genetic factors causing an increase in IL-8 during the inflammatory process. IL-8 plays a key role in the development of acute respiratory failure by activation of neutrophils, which is of basic importance for severe pancreatitis [30]. However, the results of these studies are unequivocal.…”

“…One of the potential risk factors of transition from mild to severe AP may be the production of proinflammatory cytokines, which depends on gene polymorphisms [29,30]. The studies focused on genetic factors causing an increase in IL-8 during the inflammatory process.…”