IL-6 promoter polymorphisms in rheumatoid arthritis

Genetic polymorphisms in Spanish rheumatoid arthritis patients: an association and linkage study

“…IKBL-specific PCR 16 was performed with the following primers: 5 0 -AGAAGCAGAGG-GATCCCG-3 0 for IKBL+738C, 5 0 -AGAAGCAGAGGGATCCTG-3 0 for IKBL+738 T and 5 0 -TCTCACGCAGCTCTTCCT-3 0 as common primer. IL-6 promoter was studied by a PCR-RFLP technique 3 with primers 5 0 -GGAGTCACACACTCCACCT-3 0 and 5 0 -CTGATTGGAAACCT-TATTAAG-3 0 followed by digestion with FokI and Hsp92II. A similar approach was used for FcgRIIIA genotyping, 4 with primers 5 0 -ATAAGGTCACATATTTACAGAATGGCCAAG-3 0 and 5 0 -CAGTCTCTGAAGACACATTTTTACTCCGTA-3 0 , followed by digestion with Rsa I and Sty I.…”

“…[1][2][3][4] Here we attempt to extend further some of those results and to investigate possible associations with a new set of markers. We have focussed on polymorphisms with presumed functional relevance or previously reported to be associated with an autoimmune disease.…”

“…The IL-6 promoter region contains a few well-characterised single nucleotide polymorphisms (SNPs), including a G/A transition at position À622 and a G/C transversion at position À174, which are in complete linkage disequilibrium. 3 Allele À174C has been associated with systemic juvenile chronic arthritis, and with reduced production of this cytokine. 5 A previous case-control study with Spanish RA patients has also been recently published.…”

“…5 A previous case-control study with Spanish RA patients has also been recently published. 3 IL-10 is an anti-inflammatory cytokine and it regulates the balance of Th1-Th2 responses. The human IL-10 gene promoter contains three SNPs, at positions À1082 (A/G), À819 (T/C) and À592 (C/A).…”

-174G>C interleukin-6 gene polymorphism in Tunisian patients with coronary artery disease

“…Total genomic DNA was extracted from the buffy coat layer by the salting-out procedure and was stored in TE buffer (10 mmol/L Tris-HCl, 1 mmol/L EDTA, pH 8.0). Genotyping of -174G>C (Nla III) was performed by PCR-restriction fragment length polymorphism (PCR-RFLP) as described 20. The following primers were used to amplify a 525-bp fragment containing -174 sites: 5’-GGA GTC ACA CAC TCC ACC T-3’ (sense) and 5’-CTG ATT GGA AAC CTT ATT AAG-3’ (antisense).…”