IL-5 enhances the resistance of Actinobacillus pleuropneumoniae infection in mice through maintaining appropriate levels of lung M2, PMN-II and highly effective neutrophil extracellular traps

Chen, Peiru; Bao, Chuntong; Zhu, Rining; Wang, Jun; Zhu, Jiali; Li, Ziheng; Li, Fengyang; Gu, Jingmin; Feng, Xin; Li, Na; Lei, Liancheng

doi:10.1016/j.vetmic.2022.109438

Cited by 4 publications

(7 citation statements)

References 41 publications

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“…Morphologically, FSC low and FSC high subtypes display similar characteristics to the murine neutrophil subtypes reported in a systemic inflammatory syndrome in response to intravenous Staphylococcus aureus infection 110 . In addition, infection of IL‐5 deficient mice with Actinobacillus pleuropneumoniae significantly increased PMN‐II (N2) cells in the lung 111 …”

Section: Neutrophil Functionality and Heterogeneity In Mucosal Tissuessupporting

confidence: 59%

“…110 In addition, infection of IL-5 deficient mice with Actinobacillus pleuropneumoniae significantly increased PMN-II (N2) cells in the lung. 111 The endocytosis capacity of neutrophils has recently been suggested as a factor for neutrophil heterogeneity. Two subsets of neutrophils have been characterized in murine lungs, bone marrow, peripheral blood, and spleen both under steady state and after inflammatory challenge based on endocytosis capacity.…”

Section: Neutrophil Fun C Tionalit Y and He Terog Eneit Y In Mucosal ...mentioning

confidence: 99%

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Neutrophils are gatekeepers of mucosal immunity

Silva

Kim

Moutsopoulos

2022

Immunological Reviews

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The neutrophil, a polymorphonuclear leukocyte, is the most abundant immune cell in our circulation and is considered classically as the cornerstone of the innate arm of immunity. 1 Approximately 5 × 10 10 -10 11 neutrophils are produced daily in the bone marrow, in order to maintain a steady supply of cells to the circulation and tissues. 2,3 This constant supply is necessary due to the short life span of neutrophils (6-12 hours in mice in the circulation and 5.4 days in humans and within some tissues) and occurs through a well-coordinated developmental process of granulopoiesis, which can adapt quickly and become exaggerated, when needed, to account for increased needs during infection and inflammation.Granulopoiesis or neutrophil development is initiated from hematopoietic stem cells which differentiate into multipotent progenitor (MPP) cells that do not have further potential for self-renewal.MPPs give rise to lymphoid-primed multipotent progenitors (LMPPs) and subsequently to granulocyte-monocyte progenitors (GMPs).GMPs, with the support of the granulocyte colony-stimulating factor (G-CSF), commit to neutrophil differentiation by turning into myeloblasts and subsequently transitioning through the stages of promyelocyte, myelocyte, metamyelocyte, band cell, and finally into mature neutrophils. 4 During the differentiation /maturation process, cells will have evident changes in nuclear morphology, granule content, granular protein expression, proliferative capacity, and transcriptional activity 5,6 and will express distinct cell surface markers. Specifically, primary (azurophil) granules are found at the myeloblast to promyelocyte stage. Secondary (specific) granules are detected at myelocyte and metamyelocyte stages. Tertiary (gelatinase) granules are found at the band cell stage and secretory vesicles are detected only in mature neutrophils. These granules store an arsenal of serine proteases, including elastase, myeloperoxidase, cathelicidins, defensins, and matrix metalloproteinases. 7 During maturation, the neutrophil nucleus will also mature from a round shape into a banded and then a lobulated morphology. With maturation, transcriptional, and proliferative capacity of neutrophils will also diminish, giving rise to what is considered a terminally differentiated cell state. 8

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Section: Neutrophil Functionality and Heterogeneity In Mucosal Tissuessupporting

confidence: 59%

Section: Neutrophil Fun C Tionalit Y and He Terog Eneit Y In Mucosal ...mentioning

confidence: 99%

Neutrophils are gatekeepers of mucosal immunity

Silva

Kim

Moutsopoulos

2022

Immunological Reviews

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“…After bacterial lung infection, mice were monitored daily for mortality, clinical score, and body weight loss for a period of over 5 days. A clinical score was obtained from the summation of the activity, fur, appetite, mental status, and eye secretions of the mice as described in [13]. The clinical score assessment criteria were modified as previously described in [13].…”

Section: Calculation Of Mortality Clinical Score and Body Weightmentioning

confidence: 99%

“…A clinical score was obtained from the summation of the activity, fur, appetite, mental status, and eye secretions of the mice as described in [13]. The clinical score assessment criteria were modified as previously described in [13]. Body weight loss (%) was obtained from the formula, and body weight loss (%) = 100% × (1 − (BWday x/BWday 0)).…”

Section: Calculation Of Mortality Clinical Score and Body Weightmentioning

confidence: 99%

“…Despite these numerous merits, the narrowness of the respiratory tract and pathogen-to-host specificity in mice make it challenging to establish a murine model of bacterial infections in the lung. To overcome the hurdles, intraperitoneal injection and nasal instillation have been adopted to infect mice with bacteria [11][12][13][14]. At first glance, the intraperitoneal injection of bacteria seems to be an easier method.…”

Section: Introductionmentioning

confidence: 99%

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Novel Experimental Mouse Model to Study the Pathogenesis and Therapy of Actinobacillus pleuropneumoniae Infection

Bui,

Lee,

Kuo

et al. 2024

Pathogens

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Actinobacillus pleuropneumoniae (APP) is a major cause of lung infections in pigs. An experimental mouse has the edge over pigs pertaining to the ease of experimental operation, disease study and therapy, abundance of genetic resources, and cost. However, it is a challenge to introduce APP into a mouse lung due to the small respiratory tract of mice and bacterial host tropism. In this study, an effective airborne transmission of APP serovar 1 (APP1) was developed in mice for lung infection. Consequently, APP1 infected BALB/c mice and caused 60% death within three days of infection at the indicated condition. APP1 seemed to enter the lung and, in turn, spread to other organs of the mice over the first 5 days after infection. Accordingly, APP1 damaged the lung as evidenced by its morphological and histological examinations. Furthermore, ampicillin fully protected mice against APP1 as shown by their survival, clinical symptoms, body weight loss, APP1 count, and lung damages. Finally, the virulence of two extra APP strains, APP2 and APP5, in the model was compared based on the survival rate of mice. Collectively, this study successfully established a fast and reliable mouse model of APP which can benefit APP research and therapy. Such a model is a potentially useful model for airway bacterial infections.

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Tracheal epithelial cell-exosome-derived MiR-21-5p inhibits alveolar macrophage pyroptosis to resist pulmonary bacterial infection through PIK3CD-autophagy pathway

Wang,

Gan,

et al. 2024

Life Sciences

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IL-5 enhances the resistance of Actinobacillus pleuropneumoniae infection in mice through maintaining appropriate levels of lung M2, PMN-II and highly effective neutrophil extracellular traps

Cited by 4 publications

References 41 publications

Neutrophils are gatekeepers of mucosal immunity

Neutrophils are gatekeepers of mucosal immunity

Novel Experimental Mouse Model to Study the Pathogenesis and Therapy of Actinobacillus pleuropneumoniae Infection

Tracheal epithelial cell-exosome-derived MiR-21-5p inhibits alveolar macrophage pyroptosis to resist pulmonary bacterial infection through PIK3CD-autophagy pathway

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