IL-1β Impaired Diabetic Wound Healing by Regulating MMP-2 and MMP-9 through the p38 Pathway

Dai, Jie; Shen, Junjie; Chai, Yimin; Chen, Hua

doi:10.1155/2021/6645766

Cited by 38 publications

(30 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is becoming increasingly evident that the high glucose-induced inflammation and oxidative stress may contribute to the impaired healing processes. The elevation of pro-inflammatory cytokines, such as IL-1β, has been found to impair diabetic wound healing [ 61 ]. The high glucose-induced oxidative stress also has been shown to impede the proliferation and migration of human gingival fibroblasts [ 62 ].…”

Section: Discussionmentioning

confidence: 99%

Verbascoside Protects Gingival Cells against High Glucose-Induced Oxidative Stress via PKC/HMGB1/RAGE/NFκB Pathway

Hsieh

Chu

et al. 2021

Antioxidants

View full text Add to dashboard Cite

Impaired wound healing often occurs in patients with diabetes and causes great inconvenience to them. Aside from the presence of prolonged inflammation, the accumulation of oxidative stress is also implicated in the delayed wound healing. In the present study, we tested the effect of verbascoside, a caffeoyl phenylethanoid glycoside, on the improvement of cell viability and wound healing capacity of gingival epithelial cells under high glucose condition. We showed that verbascoside attenuated the high glucose-induced cytotoxicity and impaired healing, which may be associated with the downregulation of oxidative stress. Our results demonstrated that verbascoside increased the activity of the antioxidant enzyme SOD and reduced the oxidative stress indicator, 8-OHdG, as well as apoptosis. Moreover, verbascoside upregulated the PGC1-α and NRF1 expression and promoted mitochondrial biogenesis, which was mediated by suppression of PKC/HMGB1/RAGE/NFκB signaling. Likewise, we showed the inhibitory effect of verbascoside on oxidative stress was via repression of PKC/HMGB1/RAGE/NFκB activation. Also, our data suggested that the PKC-mediated oxidative stress may lead to the elevated production of inflammatory cytokines, IL-6 and IL-1β. Collectively, we demonstrated that verbascoside may be beneficial to ameliorate impaired oral wound healing for diabetic patients.

show abstract

Section: Discussionmentioning

confidence: 99%

Verbascoside Protects Gingival Cells against High Glucose-Induced Oxidative Stress via PKC/HMGB1/RAGE/NFκB Pathway

Hsieh

Chu

et al. 2021

Antioxidants

View full text Add to dashboard Cite

show abstract

“…The pro-inflammatory IL-1ß which is grossly downregulated by HBO is involved in the regulation of the tissue remodeling enzymes MMP-2 and MMP-9. Metalloproteinases influence not only wound healing but also the regeneration of neural or muscular tissue [ 34 , 35 , 36 , 37 , 38 ]. The multiple and complex functions of MMPs are barely understood, yet the active form of MMP-9 seems to be the main factor contributing to the non-healing of diabetic ulcers [ 39 ].…”

Section: Discussionmentioning

confidence: 99%

“…Matrix metalloproteinases 2 and 9 (MMP-2/-9), that are in part regulated by levels of IL-1β, are key tissue remodeling enzymes involved in the degradation of extracellular matrix components. They have multiple overlapping activities critical for wound healing, and regeneration of skeletal muscle or neural tissue [ 36 , 37 , 38 ]. While matrix metalloproteinases (MMPs) play important roles in the pathology of wounds, the active form of MMP-9 seems to be the main factor contributing to the non-healing of diabetic ulcers [ 39 ].…”

Section: Hyperbaric Oxygen and Regenerationmentioning

confidence: 99%

Hyperbaric Oxygen Therapy and Tissue Regeneration: A Literature Survey

Lindenmann

Kamolz

Graier³

et al. 2022

Biomedicines

View full text Add to dashboard Cite

By addressing the mechanisms involved in transcription, signaling, stress reaction, apoptosis and cell-death, cellular structure and cell-to-cell contacts, adhesion, migration as well as inflammation; HBO upregulates processes involved in repair while mechanisms perpetuating tissue damage are downregulated. Many experimental and clinical studies, respectively, cover wound healing, regeneration of neural tissue, of bone and cartilage, muscle, and cardiac tissue as well as intestinal barrier function. Following acute injury or in chronic healing problems HBO modulates proteins or molecules involved in inflammation, apoptosis, cell growth, neuro- and angiogenesis, scaffolding, perfusion, vascularization, and stem-cell mobilization, initiating repair by a variety of mechanisms, some of them based on the modulation of micro-RNAs. HBO affects the oxidative stress response via nuclear factor erythroid 2-related factor 2 (Nrf2) or c-Jun N-terminal peptide and downregulates inflammation by the modulation of high-mobility group protein B1 (HMGB-1), toll-like receptor 4 and 2 (TLR-4, TLR-2), nuclear factor kappa-B (NFκB), hypoxia-inducible factor (HIF-1α) and nitric oxide (NO•). HBO enhances stem-cell homeostasis via Wnt glycoproteins and mammalian target of rapamycin (mTOR) and improves cell repair, growth, and differentiation via the two latter but also by modulation of extracellular-signal regulated kinases (ERK) and the phosphatidylinositol-3-kinase (PI3K)/protein kinase B (AKT) pathway. The HBO-induced downregulation of matrix metalloproteinases-2 and 9 (MMP-2/-9), rho-associated protein kinase (ROCK) and integrins improve healing by tissue remodeling. Interestingly, the action of HBO on single effector proteins or molecules may involve both up- or downregulation, respectively, depending on their initial level. This probably mirrors a generally stabilizing potential of HBO that tends to restore the physiological balance rather than enhancing or counteracting single mechanisms.

show abstract

“…In the sales forecast, it is a regression problem, and the output is a real number, so the loss function adopts MSE (mean square error). The calculation formula of MSE is shown in ( 4 ), where

represents the predicted value, y i represents the real value, and m is the number of samples [ 27 , 28 ].

…”

Section: Methodsmentioning

confidence: 99%

Using Convolutional Neural Network as a Statistical Algorithm to Explore the Therapeutic Effect of Insulin Liposomes on Corneal Inflammation

Liao

Jiang

et al. 2022

Computational Intelligence and Neuroscience

View full text Add to dashboard Cite

Aiming at the disadvantages of easy recurrence of keratitis, difficult eradication by surgery, and easy bacterial resistance, insulin-loaded liposomes were prepared, and convolutional neural network was used as a statistical algorithm to build SD rat corneal inflammation model and study insulin-loaded liposomes, alleviating effect on corneal inflammatory structure in SD rats. The INS/PFOB@LIP was developed by means of thin-film dispersive phacoemulsification, its structure was monitored using a transmission electron microscope, particle size and appearance potential were monitored using a Malvern particle sizer, and ultraviolet consumption spectrum was monitored using a UV spectrophotometer. The encapsulation rate, drug loading, and distribution of insulin liposomes in rat corneal inflammatory model were measured and calculated. The cytotoxicity of liposome materials was evaluated by CCK-8 assay, and the toxic effects of insulin and insulin liposomes on cells were detected. The cornea of SD rats was burned with NaOH solution (1 mol/L), and the SD rat corneal inflammation model was created. The insulin liposome was applied to the corneal inflammation model, and the therapeutic effect of insulin liposome on corneal inflammation was evaluated by slit lamp, corneal immunohistochemistry, corneal HE staining, and corneal Sirius red staining. Insulin-loaded liposomes were successfully constructed with an average particle size of (130.69 ± 3.87) nm and a surface potential of (−38.24 ± 2.57) mV. The encapsulation rate of insulin liposomes was (48.89 ± 1.24)%, and the drug loading rate was (24.45 ± 1.24)%. The SD rat corneal inflammation model was successfully established. After insulin liposome treatment, the staining area of corneal fluorescein sodium was significantly reduced, the corneal epithelium was significantly thickened, the content of corneal collagen was increased, the expression of inflammatory factors was significantly reduced, and new blood vessels (corneal neovascularization, CNV) growth was inhibited.

show abstract

IL-1β Impaired Diabetic Wound Healing by Regulating MMP-2 and MMP-9 through the p38 Pathway

Cited by 38 publications

References 35 publications

Verbascoside Protects Gingival Cells against High Glucose-Induced Oxidative Stress via PKC/HMGB1/RAGE/NFκB Pathway

Verbascoside Protects Gingival Cells against High Glucose-Induced Oxidative Stress via PKC/HMGB1/RAGE/NFκB Pathway

Hyperbaric Oxygen Therapy and Tissue Regeneration: A Literature Survey

Using Convolutional Neural Network as a Statistical Algorithm to Explore the Therapeutic Effect of Insulin Liposomes on Corneal Inflammation

Contact Info

Product

Resources

About