IL‐1α Counteract TGF‐β Regulated Genes and Pathways in Human Fibroblasts

Sydow, Anita Koskela von; Janbaz, Chris; Kardeby, Caroline; Repsilber, Dirk; Ivarsson, Mikael

doi:10.1002/jcb.25455

Cited by 8 publications

(5 citation statements)

References 41 publications

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“…Changes in cell activities and matrix components are usually associated with alteration in growth factor synthesis. TGF- β is an important tissue marker that can be produced by various cells in response to an injury and is typically produced for short periods and in limited amounts, since its action takes place in distinctive phases of the process [ 55 , 56 ]. Our results showed large amounts of TGF- β produced by groups receiving a high-fat diet and alcohol, contradicting the above statement that its production would be short and limited.…”

Section: Discussionmentioning

confidence: 99%

High-Fat Diet and Alcohol Intake Promotes Inflammation and Impairs Skin Wound Healing in Wistar Rats

Rosa

Sarandy

Novaes

et al. 2018

Mediators of Inflammation

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The wound-healing process is complex and remains a challenging process under the influence of several factors, including eating habits. As improper diets may lead to disorders such as dyslipidemia, insulin resistance, and chronic inflammation, potentially affecting the tissue ability to heal, we decided to investigate the effect of a high-fat diet and alcohol intake on the inflammatory process and skin wound healing in Wistar rats. Male rats (n = 30) were individually housed in cages with food and water ad libitum (registration number 213/2014). After anesthesia, at day 40, three circular wounds (12 mm diameter) were made on the back of each animal, which were then randomly assorted into five treatment groups: C1 (control 1)—water via gavage and standard chow diet; C2 (control 2)—water (no gavage) and standard chow diet; AL (alcohol)—water (no gavage) and alcohol (40%) via gavage and standard chow diet; HF (high fat)—water (no gavage) and high-fat diet (50%); and HF + AL (alcohol/high fat)—water (no gavage), alcohol (40%) via gavage, and high-fat diet. Animals were treated for 61 days. Every seven days, the area and the rate of wound contraction were evaluated. Tissue samples were removed for histopathological analysis and biochemical analyses. Our results showed that wound contraction was not complete in the HF + AL rats. Two specific indices of wound-healing impairment (total cell number and levels of the inflammatory cytokine TGF-β) were increased in the HF + AL rats. We also observed decreased type I and III collagen fibers in the HF, AL, and HF + AL groups and increased oxidative stress markers in the same groups. We suggest that a high-fat diet combined with alcohol intake contributed to delayed skin wound healing through increase of the inflammatory phase and promoting oxidative stress, which may have led to morphological alterations and impaired matrix remodeling.

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Section: Discussionmentioning

confidence: 99%

High-Fat Diet and Alcohol Intake Promotes Inflammation and Impairs Skin Wound Healing in Wistar Rats

Rosa

Sarandy

Novaes

et al. 2018

Mediators of Inflammation

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“…We believed that skin mast cells play a fundamental role in the regulation of inflammation due to the secretion of chemical mediators, mainly in the production of anti-inflammatory molecules that work directly on the resolution of inflammation by promoting cell proliferation, differentiation, migration and tissue remodeling [ 60 ]. TGF-β and IL-10 are considered universal mediators that can be synthesized by different cells, with positive action in the cutaneous repair process due to their capacity to accelerate matrix synthesis and tissue remodeling [ 61 , 62 ]. The results of our study corroborate this proliferative and remodeling effect, since the groups that received S pseudoquina extract showed increased amount of collagen and predominance of type I fibers, which give the tissue strength and resistance.…”

Section: Discussionmentioning

confidence: 99%

Strychnos pseudoquina modulates the morphological reorganization of the scar tissue of second intention cutaneous wounds in rats

et al. 2018

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Natural substances are used in folk medicines to treat injuries. Strychnos pseudoquina has scarring, antipyretic, and antimalarial actions. The present study aimed to analyze the effect of S. pseudoquina on cutaneous wound healing in rats. The S. pseudoquina extract was submitted to phytochemical prospection. The levels of flavonoids and total phenolic compounds in the extract were 50.7 mg/g and 2.59 mg/g, respectively. Thirty Wistar rats were individualized in cages with food and water ad libitum (registration no. 730/2014). After anesthesia, three circular wounds (12mm diameter) were made in the animals, which were randomly separated into five treatments: Sal, saline; VO, ointment vehicles (lanolin and Vaseline); SS, positive control (silver sulfadiazine 1%); LE 5, freeze-dried extract 5%; and LE 10, lyophilized extract 10%. The animals were treated with the ointment daily for 21 days. Every seven days, the area and the rate of wound contraction were evaluated. Tissue samples were removed for histopathological analysis of the number of mast cells, elastic and collagen fibers, and biochemical analyses, quantification of malondialdehyde (MDA), carbonylated proteins (PCN), superoxide dismutase (SOD), catalase (CAT), transforming growth factor β (TGF-β), Interleukin 10 (IL-10) and tumor necrosis factor (TNF). The number of mast cells, collagen and elastic fibers in the rat wounds were higher in the treatments with the plant. The extract also stimulated the activity of antioxidant enzymes, particularly SOD, presenting high levels, and maintained low levels of PCN. The TGF-β and IL-10 concentration was higher in the LE5 and LE10 treatment of the extract than in the Sal, OV and SS treatments on day 7. The ointment based on S. pseudoquina closed the wound faster and accelerated wound healing in animals.

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“…The increase in cellularity and in mast cells number can justify the increase in TGF- β expression that was observed in this study [ 15 ]. TGF- β is considered to be a universal mediator that can be synthesized by different cells with positive action in the cutaneous repair process due its anti-inflammatory effect and capacity to decrease cell migration and accelerate the matrix synthesis [ 24 , 71 ].…”

Section: Discussionmentioning

confidence: 99%

Hydroethanolic Extract of Strychnos pseudoquina Accelerates Skin Wound Healing by Modulating the Oxidative Status and Microstructural Reorganization of Scar Tissue in Experimental Type I Diabetes

Sarandy

Novaes

Xavier

et al. 2017

BioMed Research International

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The effect of topical application of ointment based on Strychnos pseudoquina hydroethanolic extract in the cutaneous wounds healing in diabetic rats was evaluated. Samples of S. pseudoquina were submitted to phytochemical prospection and in vitro antioxidant assay. Thirty Wistar rats were divided into 5 groups: Sal-wounds treated with 0.9% saline solution; VH-wounds treated with 0.6 g of lanolin cream (vehicle); SS-wounds treated with silver sulfadiazine cream (10 mg/g); ES5- and ES10-wounds treated with an ointment of S. pseudoquina extract, 5% and 10%, respectively. Fragments of wounds were removed for histological and biochemical analysis every 7 days during 21 days. ES showed equivalent levels per gram of extract of total phenols and flavonoids equal to 122.04 mg for TAE and 0.60 mg for RE. The chlorogenic acid was one of the major constituents. S. pseudoquina extract presented high antioxidant potential in vitro. ES5 and ES10 showed higher wound healing rate and higher amount of cells, blood vessels, and type III and I collagen. The oxidative stress markers were lower in the ES5 and ES10 groups, while the antioxidants enzymes levels were higher. Ointment based on S. pseudoquina extract promotes a fast and efficient cutaneous repair in diabetic rats.

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IL‐1α Counteract TGF‐β Regulated Genes and Pathways in Human Fibroblasts

Cited by 8 publications

References 41 publications

High-Fat Diet and Alcohol Intake Promotes Inflammation and Impairs Skin Wound Healing in Wistar Rats

High-Fat Diet and Alcohol Intake Promotes Inflammation and Impairs Skin Wound Healing in Wistar Rats

Strychnos pseudoquina modulates the morphological reorganization of the scar tissue of second intention cutaneous wounds in rats

Hydroethanolic Extract of Strychnos pseudoquina Accelerates Skin Wound Healing by Modulating the Oxidative Status and Microstructural Reorganization of Scar Tissue in Experimental Type I Diabetes

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