IL-17A and IL-17F induce autophagy in RAW 264.7 macrophages

Orosz, László; Papanicolaou, Elena Gouitel; Seprényi, György; Megyeri, Klára

doi:10.1016/j.biopha.2015.12.020

Cited by 34 publications

(27 citation statements)

References 23 publications

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“…COX2 , an upregulated pro‐inflammatory cytokine in periodontitis, was also reported to be increased in colon tissue of the impaired autophagy‐murine model. However, IL17F, also positively related to periodontitis, was reported to be capable of activating autophagy in murine macrophages and participate in the elimination of Mycobacterium terrae . The computer algorithm indicates that COX2 and IL17F may be potential periodontitis‐associated target genes of miR‐144‐5p, and the interactions between miRNA and mRNAs showed good base pairing.…”

Section: Discussionmentioning

confidence: 99%

“…However, IL17F, also positively related to periodontitis, was reported to be capable of activating autophagy in murine macrophages and participate in the elimination of Mycobacterium terrae. 58 The computer algorithm indicates that COX2 and IL17F may be potential periodontitisassociated target genes of miR-144-5p, and the interactions between miRNA and mRNAs showed good base pairing. Both of these two genes were negatively correlated with the expression of miR-144-5p in inflamed gingiva of chronic periodontitis patients.…”

Section: Discussionmentioning

confidence: 99%

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Assessment of microRNA‐144‐5p and its putative targets in inflamed gingiva from chronic periodontitis patients

Wang

Chen

et al. 2018

J of Periodontal Research

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Background and Objective This study aimed to discover the distinctive MicroRNAs (miRNA) functioning in the pathogenesis of periodontal inflammation, which might be potential therapy targets of chronic periodontitis. Material and Methods miRNA profiles of human inflamed gingival tissue from three previous microarrays were re‐analysed. Gingival tissues were collected for the validation of overlapping miRNAs, and a network was constructed to show regulatory connection between overlapping miRNAs and periodontitis‐associated target genes. Potential miRNAs were screened based on their expression levels and predicted target genes. Correlation analysis and binding site prediction were conducted to reveal the relationship between the potential miRNAs and their target genes. Results miR‐144‐5p, found to be upregulated in all three studies, showed the greatest upregulation (P < 0.0001). Another 16 miRNAs (10 upregulated and six downregulated) overlapped between any two of the three studies. All overlapping miRNAs had expected expression levels except for miR‐203 during validation. Ten miRNAs (six upregulated and four downregulated) were found to have periodontal inflammation‐associated targets. Cyclooxygenase 2 (COX2) and interleukin‐17F (IL17F), predicted target genes of upregulated miR‐144‐5p, showed significant decreases and were negatively correlated with miR‐144‐5p in the periodontitis group (r = −0.742 for COX2, r = −0.615 for IL17F). Conclusion This re‐analysis of miRNA signatures has implied the potential regulatory mechanism of miR‐144‐5p and its potential for exploring alternative therapeutic approaches, especially those that use miRNA delivery systems to treat chronic periodontitis. Nevertheless, further study based on larger sample size and homogenous cells is needed to reveal the exact roles of miRNAs in chronic periodontitis.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Assessment of microRNA‐144‐5p and its putative targets in inflamed gingiva from chronic periodontitis patients

Wang

Chen

et al. 2018

J of Periodontal Research

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“…IL-17A and IL-17F are important pro-inflammatory cytokines that can be expressed in many cell types (Wedebye Schmidt et al, 2013;Giles et al, 2016;Orosz et al, 2016). They can induce the secretion of pro-inflammatory cytokines, such as tumor necrosis factor alpha (TNF-a), IL-1, IL-6, IL-18, granulocyte macrophage colony, colony growth stimulating factor, chemokines, antimicrobial peptides (mucin, b defense element, and S100A-9 protein), matrix metalloproteinase 1, and NF-kB receptor activation factor ligand expression, causing tissue invasion and damage (Chabaud et al, 2001;Kordasti et al, 2009;Qu et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Association between interleukin-17 gene polymorphisms and the risk of laryngeal cancer in a Chinese population

Feng

Han

2017

Genet. Mol. Res.

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IL-17 is associated with the occurrence and development of laryngeal cancer. However, no study has reported the association between IL-17 polymorphisms and laryngeal cancer susceptibility. Therefore, we analyzed the association of three polymorphism loci (rs2275913, 197 G/A; rs3748067, 383 A/G; and rs763780, 7488 T/C) of IL-17A and IL-17F with laryngeal cancer in the Chinese population. A case-control study was performed with 325 patients and 325 controls. Polymorphisms were detected by polymerase chain reaction and sequencing methods. SPSS17.0 software was used for statistical analysis. Allele and genotype frequencies of IL-17A rs2275913 were significantly different between patients and controls (P < 0.05). Frequencies of rs2275913 (197 G/A) AA and GA+AA genotypes compared to the GG genotype were significantly higher in patients than in controls, indicating the association of these genes with laryngeal cancer susceptibility; adjusted OR values were 2.54 (1.50-4.23) and 1.62 (1.19-2.17), respectively. Furthermore, individuals with the GA+AA genotype, compared to the GG genotype, aged ≤60 years, with smoking and alcohol consumption habits, and without a family history of cancer showed a higher cancer risk (OR = 2.74, 95%CI = 1.41-5.23; OR = 2.11, 95%CI = 1.21-3.55; OR = 1.91, 95%CI = 1.02-3.70; OR = 1.99, 95%CI = 1.08-3.39, respectively). In conclusion, the rs2275913 IL-17A (197 G/A) is associated with the incidence and development of laryngeal cancer in the Chinese population, and the AA and GA+AA genotypes harbor a high laryngeal cancer risk.

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“…If DAF-16 is not phosphorylated, it will enter the nucleus and regulate the expression of antibacterial genes such as lys-7, which is related to immunity and stress [11]. It is obvious that the DAF-2/DAF-16 pathway can be used to study the antimicrobial innate immunity of nematodes [14][15][16][17]. Most reports have shown that anti-infective agents increase the survival of Staphylococcus aureus-infected worms by inducing the expression of the lys-7 gene in a DAF-16-dependent manner.…”

Section: Introductionmentioning

confidence: 99%

Brevinin-2 Drug Family—New Applied Peptide Candidates Against Methicillin-Resistant Staphylococcus aureus and Their Effects on Lys-7 Expression of Innate Immune Pathway DAF-2/DAF-16 in Caenorhabditis elegans

et al. 2018

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The issue of Staphylococcus aureus (MRSA) developing a resistance to drugs such as methicillin has long been the focus for new drug development. In recent years, antimicrobial peptides, such as small molecular peptides with broad-spectrum antibacterial activity and special antibacterial mechanism, have shown a strong medicinal potential. In particular, the Brevinin-2 family has been shown to have a significant inhibitory effect against gram-positive bacteria (G+). In this study, we researched the influence of MRSA on the behavior and survival rate of nematodes. We established an assay of Caenorhabditis elegans–MRSA antimicrobial peptides to screen for new potent anti-infective peptides against MRSA. From the Brevinin-2 family, 13 peptides that had shown strong effects on G+ were screened for their ability to prolong the lifespan of infected worms. Real-time Polymerase Chain Reaction (PCR) tests were used to evaluate the effect on the innate immune pathway dauer formation defective (DAF)-2/DAF-16 of C. elegans. The assay successfully screened and filtered out four of the 13 peptides that significantly improved the survival rate of MRSA-infected worms. The result of real-time PCR indicated that the mRNA and protein expression levels of lys-7 were consistently upregulated by being treated with four of the Brevinin-2 family. The Brevinin-2 family peptides, including Brevinin-2, Brevinin-2-OA3, Brevinin-2ISb, and Brevinin-2TSa, also played an active role in the DAF-2/DAF-16 pathway in C. elegans. We successfully demonstrated the utility of anti-infective peptides that prolong the survival rate of the MRSA-infected host and discovered the relationship between antibacterial peptides and the innate immune system of C. elegans. We demonstrated the antimicrobial effects of Brevinin-2 family peptides, indicating their potential for use as new drug candidates against MRSA infections.

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IL-17A and IL-17F induce autophagy in RAW 264.7 macrophages

Cited by 34 publications

References 23 publications

Assessment of microRNA‐144‐5p and its putative targets in inflamed gingiva from chronic periodontitis patients

Assessment of microRNA‐144‐5p and its putative targets in inflamed gingiva from chronic periodontitis patients

Association between interleukin-17 gene polymorphisms and the risk of laryngeal cancer in a Chinese population

Brevinin-2 Drug Family—New Applied Peptide Candidates Against Methicillin-Resistant Staphylococcus aureus and Their Effects on Lys-7 Expression of Innate Immune Pathway DAF-2/DAF-16 in Caenorhabditis elegans

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