IL-17 plasma levels and erythrocyte sedimentation rate on oral candidiasis animal model

Abstract

Background: A study with female animal models is important because the system immune of females is remarkably different from the male because of interaction between sex hormone and immune system. Interleukin-17 (IL-17) plays an important role in immune response toward Candida albicans (C. albicans) infection and Erythrocyte Sedimentation Rate (ERS) is an easy and sensitive test to assess the inflammation. Purpose: This study aimed to evaluate C. albicans infection, analyse the IL-17 levels and ESR in a female animal model of oral candidiasis. Methods: Female Wistar rats were used as oral candidiasis animal model. The rats divided into three groups (pre-treatment group (P0), 5th-day post-treatment group (P1) and 8th-day post- treatment group (P1). Each group consists of six rats. After the adaptation period, the P0 was sacrificed. The drinking water of the P1 and P2 was added tetracycline HCl 500mg /1L. On the day before and after inoculation C. albicans, Methylprednisolone was injected. Ten minutes before inoculation with 0.3 ml C. albicans 9.4 x 107/mL, rats were sedated by CPZ 0.7 mg IM. The rats in P1 group were sacrificed after five days and in P2 were sacrificed eight days after inoculation. The IL-17 plasma levels measured by enzyme-linked immunosorbent assay (ELISA), decreased on the 5th day but not on the 8th-day post-treatment. The obtained data were analyzed by parametric and non-parametric tests according to normality and homogeneity of the data with p<0.05. Results: The colony forming unit (CFU) of C. albicans collected over the mouth on increased almost 8-fold and on 8th-day post-treatment almost 3-fold compared with pre-treatment. The ESR increased on the 8th day but not on the 5th-day post-treatment. Conclusion: The IL-17 level was decreased on the 3rd day, ESR increased on eight days after inoculation of C. albicans in female rats’ model of oral candidiasis.

Evaluation of rat model oral candidiasis mucosal response immune

“…The elevated erythrocyte sedimentation rate may indicate increased inflammation-sensitive plasma proteins (ISPs). 17 The results of this study proved that even IL-17 levels in the oral candidiasis rat model remained at a low level on the eighth day, but the mucosal immune response in the rat model increased. Even though IL-17 is the main cytokine of the body's defence against fungi, it is well known that cytokines in the body will work together synergistically in fighting infections, including fungal infections.…”

“…15 The rat model of oral candidiasis we made in previous studies showed infection through microbiological and histopathological evaluations on the fifth and eighth day after C. albicans inoculation. 16 On day 8, there was a decrease in infection compared to day 5, but on analysis of the IL-17 plasma level, it remained lower than normal. 17 This is controversial because IL-17 is a key cytokine in the defence against fungal infections.…”

“…16 On day 8, there was a decrease in infection compared to day 5, but on analysis of the IL-17 plasma level, it remained lower than normal. 17 This is controversial because IL-17 is a key cytokine in the defence against fungal infections. 17 In this study, we directly evaluate the immune response in the infected area, the oral mucosa of the rat model.…”

“…17 This is controversial because IL-17 is a key cytokine in the defence against fungal infections. 17 In this study, we directly evaluate the immune response in the infected area, the oral mucosa of the rat model.…”