IL-17 Gene Polymorphism Is Associated with Chronic Periodontitis and Peri-implantitis in Iranian Patients: A Cross-Sectional Study

Abstract

Periodontal disease (PD) and peri-implantitis (PI) are characterized by an immune response leading to destructive inflammation. The prominent impact of genetic factors on periodontitis has been previously evaluated and IL-17 has found to play a critical role in this process. This cytokine has a controversial behavior. This study aimed at finding out whether the polymorphism of this cytokine plays a significant role in chronic periodontitis (CP) and PI or it is just a pro-inflammatory regulatory cytokine. Fresh human blood samples were obtained and three main genotypes were traced carefully. The samples were transferred into 96-well plates and sent to KBioscience Institute in the United Kingdom for genotyping the polymorphism using Competitive Allele Specific PCR (KASP) technique. SPSS version19 software and chi-square and Kruskal-Wallis tests were used for statistical differences considering p-value less than 0.05. A significant difference was detected between the three groups in terms of specific SNP studied in this experiment (P = 0.00). The CC genotype of IL17 polymorphism (rs10484879) may contribute to the pathogenesis of peri-implantitis and periodontitis. The association of IL-17 polymorphism with PI and CP is a promising finding that may help in future similar studies on other ethnicities and larger study populations.

The effect of periodontal scaling and root polishing on serum IL-17E concentrations and the IL-17A:IL-17E ratio

“…In humans, polymorphisms in the IL-17A gene have been associated with incidence of chronic periodontal diseases [3][4][5]. Furthermore, elevated levels of IL-17A have been detected in serum [6][7][8] saliva [8,9] and gingival crevicular fluid (GCF) [8,10,11] of periodontitis patients.…”

Nramp1 gene expression in different tissues of Meishan piglets from newborn to weaning

“…Nramp1 encodes a phosphoglycoprotein that functions as an ion channel during molecular transport (Cellier et al, 1995;Vidal et al, 1996). It is primarily expressed by macrophages in endothelial cell organs, including peripheral blood leukocytes, and spleen and lung tissues, which affects not only autoimmunity, but also resistance to Salmonella and various other intracellular pathogenic microorganisms (Blackwell, 1996;Lam-Yuk-Tseung et al, 2006;McDermid and Prentice, 2006;Tanaka et al, 2007;Kadkhodazadeh et al, 2016). In addition, another study found that the Nramp1 gene could influence Bacillus CalmetteGuérin (BCG) tuberculosis vaccine efficacy, indicating that Nramp1 plays an important immune regulation function with regard to pathogenic response (Badawy et al, 2013).…”

Letter from the Editor

“…In addition, popular articles in 2013 include: CD8þ T cell exhaustion during persistent viral infection is regulated independently of the virus-specific T cell receptor (Jackson et al, 2013); Measurement of suppressor activity of T CD4þCD25þ T reg cells using bromodeoxyuridine incorporation assay (Avalos-Martin, 2013); IL-17 gene polymorphism is associated with chronic periodontitis and periimplantitis in Iranian Patients: A cross-sectional study (Kadkhodazadeh et al, 2013); Biomarkers of lung injury in critical care medicine: Past, present, and future (Tunceroglu et al, 2013); Reconstitution and phenotype of tregs in CMV reactivating patients following allogeneic hematopoietic stem cell transplantation (Velaga et al, 2013); Immunological studies of reactive oxygen species damaged catalase in patients with Systemic Lupus Erythematosus: Correlation with Disease Activity Index (Al-Shobaili et al, 2013); Role of TLR-2 and fungal surface antigens on innate immune response against Sporothrix schenckii (Negrini et al, 2013); Intracellular CTLA4 and regulatory T cells in patients with laryngeal squamous cell carcinoma (Erfani et al, 2013); Protection Against Cyclophosphamide-Induced Myelosuppression by ZPDC Glycoprotein (24 kDa) (Lee & Lim, 2013).…”

“…T-cell IFN-β IFN-γ Souza and Lerner, 2013 In the four years prior to this thematic, five articles which discuss bone or inflammation and periodontal disease were published in Immunological Investigations: Development of a high-specificity enzyme-linked immunosorbent assay (ELISA) system for the quantification and validation of intact rat osteocalcin (Funaoka et al, 2010); The clinical significance of OPG/ sRANKL ratio in thalassemia patients suffering from osteopenia or osteoporosis in Egyptian patients (Salah, 2010); Myeloperoxidase as inflammatory marker of periodontal disease: experimental study in rats (Gomes et al, 2009); IL-17 gene polymorphism is associated with chronic periodontitis and periimplantitis in Iranian patients: A cross-sectional study (Kadkhodazadeh et al, 2013); and TLR4 and IL-18 gene variants in chronic periodontitis: impact on disease susceptibility and severity (Noack et al, 2009). These articles have whet our appetite for understanding the puzzle of the array of interactions of bone cells with cells and factors of both the innate and the acquired immune system.…”