II. The Diagnostic Application of Fluorescent Antibodies

Coons, Albert H.

doi:10.1159/000160655

Cited by 7 publications

(2 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…III. IMMUNOFLUORESCENT STAINING OF MICROORGANISMS An extensive bibliography of this subject may be found in Coons' recent review on the diagnostic applications of the method (29). The objectives here are to review the most significant contributions that have been made since Coons' review of 1956 (24) and to discuss the literature in terms of the various ways in which immunofluorescence has been brought to bear on microbiological problems.…”

mentioning

confidence: 99%

Immunofluorescent Staining: The Fluorescent Antibody Method1

Beutner¹

1961

Bacteriological Reviews

View full text Add to dashboard Cite

marize the major original contributions made by this method since 1956 (older papers are reviewed only as they relate to the more recent developments); (c) to relate immunofluorescence to the classic concepts of immunology. A. Fluorescent Antibody Labeling Compounds which emit light in one wave length range when illuminated by light of a shorter wave length range are fluorescent. Most fluorescent compounds that have been studied are excited by light in the ultraviolet or near ultraviolet region and emit light in the visible range. Fluorescent dyes are called fluorochromes. The coupling of fluorochromes to antibodies forms the basis of staining with labeled antibody. Briefly, three methods of coupling fluorescent dyes to proteins have been utilized to prepare conjugates for IF staining. (As used here the term "conjugates" refers to fluorochrome-labeled proteins.) According to the original procedure of Coons, Creech, Jones, and Berliner (30) an isocyanate derivative of fluorescein is prepared by reaction of amino group of the fluor with phosgene. The isocyanate reacts with free amino groups of proteins to form a carbamido linkage: 49

show abstract

mentioning

confidence: 99%

Immunofluorescent Staining: The Fluorescent Antibody Method1

Beutner¹

1961

Bacteriological Reviews

View full text Add to dashboard Cite

show abstract

“…θέματος τούτου πολύ διαφωτιστικά εΐναι τα άρθρα τώνCoons (1959),Beutner (1961) και ή μονογραφία του Cherry και συν.(1960). Δια της μεθό δου ταύτης θεωρητικώς δύναται τις ν' απόδειξη τήν ΰπαρξιν μικροοργανι σμών άπ' ευθείας έπί παρασκευασμάτων εκ παθολογικών υλικών, άλλ' εν τη πράξει τούτο μόνον εν μέρει δύναται να έφαρμοσθη.…”

unclassified

Η Σημασια Της Εμμεσου Μεθοδου Του Ανοσοφθοριμου Εις Την Οροδιαγνωστι- Κην Των Σαλμονελλωσεων

Πιτσιγαυδακησ¹

View full text Add to dashboard Cite

Εις την επιμελήτριαν του 'Εργαστηρίου κ. Δέσποιναν Πετροπούλου -Αεωτσάκη εκφράζω θερμός ευχαριστίας δια την συμπαράστασίν της.Εις τον επιμελητήν τοΰ 'Εργαστηρίου κ. Γεώργιον Αηλαναν, δστις δια των χρησίμων συμβουλών και οδηγιών του προς άρτιωτεραν σύνταξιν της παρούσης εργασίας τα μέγιστα συνέβαλεν, εκφράζω τάς θερμός μου ευχαριστίας.'Ωσαύτως ευχαριστώ θερμώς την παρασκευάστριαν κ. Χατζηε^θεριάδου -Τζανετάκου δια την βοήθειάν της ε/ç το τεχνικον μέρος της εργασίας.

show abstract

Quantitative Untersuchungen �ber den direkten und indirekten fluoreszenz-serologisehen Nachweis der Komplementbindung an Enterovirus-Antik�rper-Komplexe

Mueller¹

1961

Archiv f Virusforschung

View full text Add to dashboard Cite

II. The Diagnostic Application of Fluorescent Antibodies

Cited by 7 publications

References 21 publications

Immunofluorescent Staining: The Fluorescent Antibody Method1

Immunofluorescent Staining: The Fluorescent Antibody Method1

Η Σημασια Της Εμμεσου Μεθοδου Του Ανοσοφθοριμου Εις Την Οροδιαγνωστι- Κην Των Σαλμονελλωσεων

Quantitative Untersuchungen �ber den direkten und indirekten fluoreszenz-serologisehen Nachweis der Komplementbindung an Enterovirus-Antik�rper-Komplexe

Contact Info

Product

Resources

About