IgM-class antibodies to TT virus (TTV) in patients with acute TTV infection

Objective: To determine the prevalence and clinical impact of transfusion-transmitted virus (TTV) DNA in patients with chronic liver diseases in the Southeast Anatolia region of Turkey where hepatitis B and C viral infections are endemic. Subjects and Methods: Patients diagnosed with chronic liver disease by clinical, biochemical and histologic means were enrolled in the study. Serum samples of 60 patients (19 males, 41 females) with chronic liver diseases, and of 45 healthy volunteer blood donors as a control group were collected. The chronic liver disease group consisted of 11 patients with hepatitis B, 44 with hepatitis C and 5 with chronic liver disease of unknown etiology. Presence of TTV DNA was investigated by the polymerase chain reaction. Using a scoring system histological grading of inflammation and staging of fibrosis were performed only in the chronic hepatitis C group. Results: TTV DNA was detected in 47 (78%) patients with chronic liver disease and 5 (11%) volunteers in the control group. The difference was statistically significant (p < 0.001). Ten of the 11 (91%) patients with hepatitis B, 32 of 44 (73%) of those with hepatitis C-related chronic liver disease, and 5 of 5 (100%) of the patients with cryptogenic liver disease were positive for TTV DNA. Conclusion: TTV is highly prevalent in patients with chronic liver diseases in Southeast Anatolia, Turkey but no pathogenic effect attributable to TTV infection was detected.

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“…Newly defined IgM class antibodies may be important in further evaluation of this group of patients [26].…”

Section: Discussionmentioning

confidence: 99%

High Prevalence of Transfusion-Transmitted Virus Infection in Patients with Chronic Liver Diseases in an Endemic Area of Hepatitis B and C Virus

Savaş

Güney²,

Kadayıfçı³

et al. 2003

Med Princ Pract

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“…TTV specific antibodies have been detected in the sera of infected individuals by Tsuda et al [102]. Tsuda et al used the technique of immunoprecipitation to establish, that after the clearance of TTV DNA, short term antibodies IgM appeared in the blood and after them long lived IgG antibodies showed up [102].…”

Section: Ttv Immunologymentioning

confidence: 99%

“…TTV immunocomplexes have been detected in the sera of TTV positive individuals [102,105]. These immunocomplexes have been found in case of prolonged infections but not in acute infections [31].…”

Section: Ttv Immunologymentioning

confidence: 99%

Current and Future Prospects of Torque Teno Virus

Elsaie¹

2013

J Vaccines Vaccin

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Human health has a significant importance and it is directly affected by a number of factors like different diseases including microbial and viral infections. Up till now we have paid attention to the disease causing viruses and pathogens and less has been investigated about the usefulness of normal viral flora. One such orphan virus is Torque Teno Virus (TTV). The virus is present in healthy as well as diseased population, indicating its high prevalence. The virus is ubiquitous to human body as it is present in almost all tissues and organs and this is the property that can be utilized for targeted drug delivery. Another potentially useful property of this virus is TTV derived Apoptosis Inducing protein that can be a hope for the affectees.

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“…The reports from few other studies have shown the presence of IgM antibodies [8]. However, these were detected to be the short lived antibodies [37,38] and could not be frequently found in sera by different techniques used. Possibly, the screening of sera samples in this study had a similar problem where presence of IgM cannot be ruled out.…”

Section: Discussionmentioning

confidence: 85%

Expression of TTV-ORF2 Protein for Detection of Anti-TTV IgG Antibodies in Human Sera

Singh¹,

Singh²,

Mankotia³

et al. 2013

AID

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The present study describes the cloning and expression of ORF-2 region of TTV genome and the use of expressed peptide in developing immunoassay for detection of anti-TTV antibodies in serum. Presence of TTV-DNA in serum was detected by PCR amplifying N-22 region of ORF-1 of TTV genome. This was followed by genotyping of TTV by RFLP using N-22 amplicon. Using genotype-1 positive serum as the source of TTV, the ORF-2 region was amplified by PCR and subsequently cloned and expressed in pET-19b vector. The expressed protein, identified as 20 kDa protein on SDS-PAGE gel, was purified by affinity chromatography and then used as antigen to develop western blot assay for detection of anti-TTV antibodies in serum. Analysis of sera for anti-TTV antibodies and their comparison with presence of TTV-DNA, produced encouraging results. There was a good relation between presence of anti-TTV and TTV-DNA in these sera samples. Anti-TTV antibodies could be detected in all TTV-DNA positive sera irrespective of the presence of TTV-genotype. This investigation demonstrates that ORF-2 peptide may be used in developing immunoassay for identification of TTV infection.

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IgM-class antibodies to TT virus (TTV) in patients with acute TTV infection

Cited by 27 publications

References 16 publications

High Prevalence of Transfusion-Transmitted Virus Infection in Patients with Chronic Liver Diseases in an Endemic Area of Hepatitis B and C Virus

High Prevalence of Transfusion-Transmitted Virus Infection in Patients with Chronic Liver Diseases in an Endemic Area of Hepatitis B and C Virus

Current and Future Prospects of Torque Teno Virus

Expression of TTV-ORF2 Protein for Detection of Anti-TTV IgG Antibodies in Human Sera

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