IgG‐Type Cold Agglutinins in Children and Corresponding Antigens

Roelcke, D.; Anstee, David J.; Jungfer, H.; Nützenadel, W.; Webb, A. J.

doi:10.1111/j.1423-0410.1971.tb00433.x

Cited by 58 publications

(31 citation statements)

References 32 publications

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“…In one patient (JSh), the serolo gy was unusual in that the IgG autoanti body reacted much better at room temper ature than at 37 °C; a few other examples of IgG cold autoantibodies causing hae molysis in children have been noted [29,30],…”

Section: Distribution O F Cases Clinical and Laboratory Featuresmentioning

confidence: 99%

Autoimmune Haemolysis in Childhood and Adolescence

et al. 1984

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The clinico-pathological features of 42 children with autoimmune haemolysis are described. Over 65 % of cases were seen before their 5th birthday. In this group males predominated by the ratio of 2.5:1, but in the older children both sexes were equally affected. The incidence decreased from 1 in 188 × 10³ in young males to 1 in 1,780 × 10³ in children over 10. Cases were classified serologically. Of particular note was the frequency of Donath-Landsteiner haemolysis which equalled that due to warm autoantibodies; together these groups made up 79% of the total cases. Most haemolytic episodes followed an acute infection. This was frequently mild and often involved the upper respiratory tract; in only 2 patients was haemolysis associated with underlying collagenosis. Typically there was a sudden onset of pallor and malaise; jaundice, splenomegaly and hepatomegaly were found in about half the subjects. Haemoglobinuria was characteristic of Donath-Landsteiner haemolysis. The illness was severe, with Hb levels falling below 6.0 g/dl in 28 patients. Prednisolone, blood transfusion and, where indicated, antibiotics were usually effective in treating the illness, with splenectomy reserved for cases where this treatment was unsatisfactory. In several individuals no treatment was required. Recovery was rapid, and complete recovery occurred in 83 % of patients, usually within 6 months. Although 2 patients died, a generally optimistic prognosis can be given, particularly in the absence of an underlying chronic disorder.

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Section: Distribution O F Cases Clinical and Laboratory Featuresmentioning

confidence: 99%

Autoimmune Haemolysis in Childhood and Adolescence

et al. 1984

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“…Details of this case are being prepared for publication (Lockyer and Webb). An exceptional hightitre cold agglutinin which as found to have a new specificity anti-Pra by Roelke et al [8] was confined to the IgG fraction, behaving similary to one of the enzyme auto-antibodies tested. Two anti-i cases had activity in both IgG and IgM fractions in agreement with Capra et al [2], Both were macro-globulinaemic, precipitated heavily in distilled water and caused abnormal shrinkage of the gel.…”

Section: Discussionmentioning

confidence: 91%

“…IgG antibodies can cause agglutination in saline medium, for example anti-D and anti-Kell [6,7] and anti-Pra [8], We had thought that the saline reaction of D--/D--cells with some incomplete anti-D sera was brought about by IgG antibody due to a reduced zeta-potential caused by cell membrane abnormalities. It was therefore surprising to find that the specific saline activity eluted with the IgM and IgA molecules from the gel.…”

Section: Discussionmentioning

confidence: 99%

A 30-Minute Preparative Method for Isolation of IgG from Human Serum

Webb¹

1972

Vox Sanguinis

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IgG antibody activity may be isolated in good yield from non-dialysed serum in under 30 min. The method requires the mixing of serum with prepared DEAE Sephadex A-50 for a few minutes, followed by centrifugation and aspiration of the IgG-rich fraction. Immuno-electrophoresis, anti-IgG neutralisation and partition of mixed anti-red cell antibodies suggests good separation of IgG from other immunoglobulin classes.

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“…The purified material strongly inhibited anti-P, antisera (Table 1) but did not inhibit the agglutination of normal human red cells by antisera of the specificities : anti-A, anti-B, anti-H, anti-I (6 sera examined), anti-i (3 sera examined), anti-P (2 sera examined), anti-Pr,, anti-Pr,, anti-Pr, [21]. (Table 4).…”

Section: Properties Of Purified €'-Active Materialsmentioning

confidence: 92%

Structural Aspects of Heterologous Blood‐Group‐P₁ Substance

Anstee

Pardoe

1973

European Journal of Biochemistry

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Blood-group-P, substance from sheep hydatid-cyst fluid was purified by a combination of gel filtration through Sepharose 6B and preparative polyacrylamide-gel electrophoresis. The resultant glycoprotein contained D-galactose ; D-glucose ; N-acetyl-D-ghcosamhe and N-acetyl-D-galactosamine in molar ratios 5.2 : 1 : 2 : 2; unidentified carbohydrate was also present. The amino-acid composition resembled that of vertebrate A, B, H and Lewis active glycoproteins.Prolonged incubation of P, substance with coffee bean ol-galactosidase resulted in the liberation of 17-240/, of the total D-galactose present and a reduction in blood-group-P, inhibitory activity. The serological activity of the glycoprotein was unaffected by treatment with pronase or papain.P, substance has receptors for the agglutinins from Salmo trutta, Clupm harengus, Soja hispida, Maclura aurantiaca and Triticum vulgaris. Some loss of receptor for Soja hispida and Maclura aurantiaca occurred after ol-D-galactosidase treatment.The structural immunochemistry of the P bloodgroup system of human erythrocytes has not yet been elucidated, although, in common with the ABO system, blood-group-active substances of heterologous origin are available and have provided insight into cross-reactive antigens. Cameron and Staveley [I] reported that hydatid-cyst fluid from sheep carrying tapeworm parasites (Echinococcus granulosus) had potent P, inhibitory activity. Freezedried hydatid-cyst fluid was extracted with phenol, by the procedure used to extract blood-group materials from human ovarian-cyst fluid. The resultant glycoprotein was reported to be similar in composition to human ovarian-cyst blood-group substances [2].Serological studies demonstrated strong specific PI inhibitory activity and indicated the importance of a-D-galactosyl groupings in P, blood-group activity We are reporting additional observations relating to the structure and serological characteristics of the P, active glycoprotein. ~31.

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IgG‐Type Cold Agglutinins in Children and Corresponding Antigens

Cited by 58 publications

References 32 publications

Autoimmune Haemolysis in Childhood and Adolescence

Autoimmune Haemolysis in Childhood and Adolescence

A 30-Minute Preparative Method for Isolation of IgG from Human Serum

Structural Aspects of Heterologous Blood‐Group‐P₁ Substance

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IgG‐Type Cold Agglutinins in Children and Corresponding Antigens

Cited by 58 publications

References 32 publications

Autoimmune Haemolysis in Childhood and Adolescence

Autoimmune Haemolysis in Childhood and Adolescence

A 30-Minute Preparative Method for Isolation of IgG from Human Serum

Structural Aspects of Heterologous Blood‐Group‐P1 Substance

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Structural Aspects of Heterologous Blood‐Group‐P₁ Substance