A 30-Minute Preparative Method for Isolation
of IgG from Human Serum

Webb, A.J.

doi:10.1159/000466556

Cited by 11 publications

(10 citation statements)

References 9 publications

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“…Rat IgG was prepared from rat serum using DEAE Sephadex A50 (course) following the method of Webb (1972). The yield of this method was 7-8 mg/ml.…”

Section: Preparation Of Labelled Proteinmentioning

confidence: 99%

The transmission of −125‐I‐labelled immunoglobulin G by proximal and distal regions of the small intestine of 16‐day‐old rats.

Morris¹

1975

The Journal of Physiology

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SUMMARY1. Standard doses of 1251-labelled rat IgG were injected into the intestinal lumen of rats aged 16 days, and their sera were sampled 2 and 3 hr later. High concentration quotients were obtained after injection into the proximal small intestine, whereas very little immunoglobulin was transmitted from doses injected into the terminal 20 cm of the small intestine.2. The villi of the terminal 18-20 cm of the small intestine of 16-day-old rats, the region from which very little transmission of IgG occurred, were lined by tall columnar absorptive cells with very large supra-nuclear vacuoles. The extent of the terminal intestine, in which this cell type predominated in the absorptive epithelium, varied with age. The importance of defining the precise location of the region of the intestine under examination is stressed.3. The experimental results and the histological observations are discussed in relation to (a) the results which have been obtained using PVP, which is unsuitable as an indicator of immunoglobulin transport in the rat and (b) the histological composition of the absorptive epithelium and the maturation changes which affect the epithelium between 18 and 21 days.

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“…Rat IgG was prepared from rat serum using DEAE Sephadex A50 (course) following the method of Webb (1972). The yield of this method was 7-8 mg/ml.…”

Section: Preparation Of Labelled Proteinmentioning

confidence: 99%

The transmission of −125‐I‐labelled immunoglobulin G by proximal and distal regions of the small intestine of 16‐day‐old rats.

Morris¹

1975

The Journal of Physiology

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“…Preparation of labelled protein Rat IgG was prepared from rat serum using DEAE Sephadex A 50 (coarse) following the method of Webb (1972). The yield of the method was 7-9 mg/ml.…”

Section: Animalsmentioning

confidence: 99%

The absorption of ¹²⁵I‐labelled immunoglobulin G by different regions of the gut in young rats

Morris¹,

Morris²

1974

The Journal of Physiology

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SUMMARY1. 125I-labelled homologous IgG was injected into different regions of the small intestine of rats aged 12, 16, 18, 20 and 22 days. At 12 days the proximal and middle regions of the intestine readily absorbed globulin and transmitted it to the circulation. The distal region of the intestine transmitted little to the circulation at all ages tested.2. The intestine loses its ability to transmit globulin to the circulation in a distal-proximal direction. At 16 and 18 days the ability of the middle region had declined significantly, and this decline continued so that little globulin was transmitted from this region at 20 and 22 days.3. The proximal intestine retained the ability to transmit globulin to the circulation in significant amounts up to 20 days.4. There is a close negative correlation between body weight and total radioactivity of the sera of rats which had received doses of labelled globulin into the proximal and middle regions of the intestine. There was no such correlation after injection into the distal intestine -suggesting a restricted throughput of radioactive material by the absorptive cells of this region.5. These results are discussed in the context of the termination of antibody absorption, and in relation to the results obtained using polyvinyl pyrrolidone.

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“…The separation was carried out by means of a batch procedure [2] and not by chromatography. The technique corresponded to a modification of the batch pro cedure as given by Webb [14].…”

Section: Resultsmentioning

confidence: 99%

On the Problems of Efficacy of Separation of IgM and IgG by Different Methods

Sugg¹,

Schneider²,

Frösner³

et al. 1979

Vox Sanguinis

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Using ultracentrifugation, gel filtration, and ion exchange, an attempt was made to completely separate immunoglobulin IgM and IgG present in pooled human serum of blood group O. The quality of separation was studied by testing IgG fractions for the presence of IgM by a sensitive detection method. It is demonstrated by rheophoresis that small amounts of IgM always remain detectable in the IgG fraction. This is of clinical importance because conclusions on the presence of specific antibody of different Ig classes are usually drawn under the assumption of complete separation. The control of the efficacy of the separation process using a sensitive detection method for Ig of different classes is recommended.

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A 30-Minute Preparative Method for Isolation of IgG from Human Serum

Cited by 11 publications

References 9 publications

The transmission of −125‐I‐labelled immunoglobulin G by proximal and distal regions of the small intestine of 16‐day‐old rats.

The transmission of −125‐I‐labelled immunoglobulin G by proximal and distal regions of the small intestine of 16‐day‐old rats.

The absorption of ¹²⁵I‐labelled immunoglobulin G by different regions of the gut in young rats

On the Problems of Efficacy of Separation of IgM and IgG by Different Methods

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A 30-Minute Preparative Method for Isolation of IgG from Human Serum

Cited by 11 publications

References 9 publications

The transmission of −125‐I‐labelled immunoglobulin G by proximal and distal regions of the small intestine of 16‐day‐old rats.

The transmission of −125‐I‐labelled immunoglobulin G by proximal and distal regions of the small intestine of 16‐day‐old rats.

The absorption of 125I‐labelled immunoglobulin G by different regions of the gut in young rats

On the Problems of Efficacy of Separation of IgM and IgG by Different Methods

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The absorption of ¹²⁵I‐labelled immunoglobulin G by different regions of the gut in young rats