IgG subclasses and DNA detection of HHV‐6 and HHV‐7 in healthy individuals

Biganzoli, Patricia; Ferreyra, Leonardo; Sicilia, Paola; Carabajal, C. C.; Frattari, Susana Ruiz de; Littvik, Ana; Nates, Silvia; Pavan, Jorge Victorio

doi:10.1002/jmv.21880

Cited by 18 publications

(22 citation statements)

References 33 publications

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“…It is known that HHV-6 and HHV-7 herpesviruses can be detected in healthy individuals who are asymptomatic [34] as exanthema subitum can be caused by a primary infection by HHV-6 and, less frequently, by HHV-7 [35,36]. The rate of oral excretion of HHV-6 in the healthy population is low (i.e.…”

Section: Discussionmentioning

confidence: 99%

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Oral shedding of human herpesviruses in patients undergoing radiotherapy/chemotherapy for head and neck squamous cell carcinoma is not affected by xerostomia

Palmieri

Ornaghi

Martins

et al. 2018

Journal of Oral Microbiology

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Background: Xerostomia is a very relevant and frequent complication of radiotherapy, causing the irradiated oral mucosa to be affected by bacterial, fungal and viral infections. Objective: The objective of this study was to evaluate a possible relationship between oral shedding of human herpesviruses and xerostomia in patients with squamous cell carcinoma of head and neck submitted to radio/chemotherapy. Methods: In this study, oral rinse samples were collected weekly from 20 patients during radiotherapy. The samples were submitted to PCR and enzymatic digestion for detection of human herpesviruses. Xerostomia was evaluated according to the Seminars in Radiation Oncology criteria. Results: There was a higher frequency of grade 1 xerostomia (51.4%), observed first in the 1st week of radiotherapy. In the 4th week of radiotherapy, all patients presented some degree of xerostomia. Analysis of herpesviruses showed oral shedding of EBV, HHV-6 and HHV-7 in all weeks. Considering all the periods, the highest frequency was in patients with EBV excretion (55.0%), which was significantly higher than that of other viruses. Conclusion: We observed that oral shedding of herpesviruses was not affected by xerostomia as there was a progression in their excretion, even with the evolution of xerostomia. This suggested that there is a local replication in the oral cavity that is not completely dependent of salivary excretion.

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Section: Discussionmentioning

confidence: 99%

“…The rate of oral excretion of HHV-6 in the healthy population is low (i.e. about 10%), while oral shedding rates of HHV-7 can vary from 12.6% to 90% depending on the population group [34,37]. HHV-6 and HHV-7 can be observed not only in healthy patients, but also in immunocompromised ones.…”

Section: Discussionmentioning

confidence: 99%

Oral shedding of human herpesviruses in patients undergoing radiotherapy/chemotherapy for head and neck squamous cell carcinoma is not affected by xerostomia

Palmieri

Ornaghi

Martins

et al. 2018

Journal of Oral Microbiology

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“…56 No HHV-6 DNA is found in the plasma of healthy donors, other than those with ciHHV-6. [57][58][59] Similarly, HHV-6 DNA is only found in the CSF in cases of active infection. The only exception would be a ciHHV-6 patient where HHV-6 DNA can be detected because of pleocytosis or contamination of the sample at the time of lumbar puncture.…”

Section: Discussionmentioning

confidence: 99%

HHV-6 encephalitis in umbilical cord blood transplantation: a systematic review and meta-analysis

Scheurer

Pritchett

Amirian

et al. 2012

Bone Marrow Transplant

108

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Reactivation of human herpesvirus-6 (HHV-6) frequently occurs following hematopoietic SCT (HSCT), and has been associated with clinical consequences in many patient populations. HHV-6 reactivation and HHV-6 encephalitis seem to occur more frequently in patients undergoing HSCT with cord blood (CB) as the stem cell source. We have conducted a systematic literature review and meta-analysis to investigate the clinical significance of this correlation. A systematic review of publications indexed in PubMed was performed for HSCT studies published over the past 10 years that fit inclusion criteria. Data on prevalences of HHV-6 reactivation and HHV-6 encephalitis post HSCT were abstracted from 19 papers. Meta-analyses were conducted to calculate combined prevalence estimates. The prevalences of HHV-6 reactivation and encephalitis were compared among CB vs non-CB HSCT. Prevalences of HHV-6 reactivation and HHV-6 encephalitis were significantly higher in patients receiving CB as the stem cell source than in patients receiving another stem cell source (72.0% vs 37.4%, Po0.0001; 8.3% vs 0.50%, Po0.0001, respectively). HHV-6 reactivation and HHV-6 encephalitis are significant complications in the post-HSCT setting, particularly in patients receiving CB as the stem cell source. Thus, patients undergoing umbilical CB transplantation should be closely monitored for HHV-6 reactivation.

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“…The modern serological methods detect the presence of IgM and IgG [51] usually by ELISA. This technique does not detect the virus in early stages of infection, as antibodies are produced by the host only after this phase.…”

Section: Serological Methodsmentioning

confidence: 99%

“…It is fast, direct and sensitive, and is considered a quantitative technique for viral load [54,55]. CMV antigenemia is one of the earlier tests with positive results [17,25,[51][52][53][54][55][56][57][58] and becomes positive on average 9-18 days before establishment of the disease. It has been widely used for the early detection of active infection in organ transplant recipients [17,24,25,36,56].…”

Section: Immunofluorescence (If)mentioning

confidence: 99%