IgG, IgM and IgA antibodies against the novel polyprotein in active tuberculosis

Xia, Feng; Yang, Xinglun; Xiu, Bingshui; Qie, Shuang; Dai, Zhenhua; Chen, Kun; Zhao, Ping; Zhang, Li; Nicholson, Russell A.; Wang, Guohua; Song, Xi; Zhang, Heqiu

doi:10.1186/1471-2334-14-336

Cited by 23 publications

(21 citation statements)

References 36 publications

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“…Our data suggest that patients with positive cultures show both IgA and IgG positive profiles. It has been reported that patients with active disease show a strong IgG response but poor IgA and IgM responses [8,14,15]. Although some studies show better values of diagnostic sensitivity for IgA than for IgG [16][17][18], we found a clear correlation between active disease and IgA and IgG.…”

Section: Discussioncontrasting

confidence: 53%

Comparative analysis of antibodies and lymphocytes in pulmonary tuberculosis patients with negative and positive cultures

Cázares-Sosa

Hernández-Solís

Andrés-Dionicio

et al. 2019

J Infect Dev Ctries

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Introduction: The sputum smear or the culture are the definitive diagnosis of pulmonary tuberculosis. Only a fraction of clinical patients are culture-confirmed. Methodology: A total of 24 clinical cases (40 ± 14 years old) with positive smear and negative co-morbidity were studied. Cases were selected from 600 patients who attended the pneumology service over two years. A sputum sample was cultured in Löwenstein-Jensen medium with consequent amplification of the rrnA V2 promoter, the differentiation region 4, and the IS6110 insertion sequence of Mycobacterium tuberculosis. After the culture result, the patients were divided into negative (n = 14) or positive (n = 10) culture groups. In addition, 30 samples from healthy donors (45 ± 10 years) were studied. The numbers of CD4, CD8 and CD19 lymphocytes were determined by flow cytometry. Levels of IgA and IgG to M. tuberculosis were measured by ELISA. Results: IgG and IgA levels were detected in patients with positive culture, while only IgA was found in patients with negative cultures. The lymphocyte populations in the two groups were similar. The presence of a pleural apical cap was found more frequently in patients with negative- (57%) than with positive cultures (10%). Conclusions: The isotype profile in patients with positive cultures was both IgA and IgG positive, while in patients with negative culture, only IgA was found. The results will contribute to improve the diagnostic algorithm and appropriate treatment of patients with clinical tuberculosis. Further studies are needed to determine if this profile is predictive of the outcome of isolation.

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Section: Discussioncontrasting

confidence: 53%

Comparative analysis of antibodies and lymphocytes in pulmonary tuberculosis patients with negative and positive cultures

Cázares-Sosa

Hernández-Solís

Andrés-Dionicio

et al. 2019

J Infect Dev Ctries

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“…Using immunodominant fragments instead of the whole protein for antibody detection has been well established in our lab for several years. Previously, we have successfully cloned many antigen fragments of immunodominant epitopes and published several papers about the application of this method to identify biomarkers for disease diagnosis [ 17 , 21 , 28 ]. There are two main advantages of using antigen fragments over using the whole protein.…”

Section: Discussionmentioning

confidence: 99%

“…The active PTB was diagnosed based on clinical symptoms, including coughing, fever, coughing up of blood, and pulmonary fibrocavity infiltrates on chest radiograph. For the suspected TB cases, sputum smear and culture test was performed as reported previously [ 21 ]. Final TB diagnosis was based on the result of sputum smear and culture test as well as symptomatic improvement after anti-TB therapy.…”

Section: Methodsmentioning

confidence: 99%

“…The diagnostic value of the five antigens with indirect ELISA assay was evaluated by the receiver operating characteristic (ROC) curve analysis. In ROC curve, the optimal operating point (OOP) was determined via Youden's index according to the method reported previously [ 21 ]. Briefly, the sensitivity and specificity of each operating point in the ROC curve were automatically calculated using GraphPad Prism 4.0 software, followed by calculation of Youden's index of each point (Youden's index = sensitivity + specificity − 1).…”

Section: Methodsmentioning

confidence: 99%

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Identification of Novel RD1 Antigens and Their Combinations for Diagnosis of Sputum Smear−/Culture+ TB Patients

Liu¹,

Qie²,

et al. 2016

BioMed Research International

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Rapid and accurate diagnosis of pulmonary tuberculosis (PTB) is an unresolved problem worldwide, especially for sputum smear− (S−) cases. In this study, five antigen genes including Rv3871, Rv3874, Rv3875, Rv3876, and Rv3879 were cloned from Mycobacterium tuberculosis (Mtb) RD1 and overexpressed to generate antigen fragments. These antigens and their combinations were investigated for PTB serodiagnosis. 298 serum samples were collected from active PTB patients, including 117 sputum smear+ (S+) and sputum culture+ (C+) cases, 101 S−/C+ cases, and 80 S−/C− cases. The serum IgG levels of the five antigens were measured by ELISA. Based on IgG levels, the sensitivity/specificity of Rv3871, Rv3874, Rv3875, Rv3876, and Rv3879 for PTB detection was 81.21%/74.74%, 63.09%/94.78%, 32.21%/87.37%, 62.42%/85.26%, and 83.56%/83.16%, respectively. Furthermore, the optimal result for PTB diagnosis was achieved by combining antigens Rv3871, Rv3876, and Rv3879. In addition, the IgG levels of Rv3871, Rv3876, and Rv3879 were found to be higher in S−/C+ PTB patients than in other PTB populations. More importantly, combination of the three antigens demonstrated superior diagnostic performance for both S−/C+ and S−/C− PTB. In conclusion, the combination of Rv3871, Rv3876, and Rv3879 induced higher IgG response in sputum S−/C+ PTB patients and represents a promising biomarker combination for diagnosing of PTB.

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“…Notably, while serodiagnosis of TB by combining immunoglobulin classes was suggested as a useful point-of-care test in South Africa (12), poor performance of the commercial serological tests for diagnosing pulmonary TB was reported in India (13), and to date, no commercial serodiagnostic tests have demonstrated acceptable sensitivity and specificity for routine laboratory use. In previously developed tests, these have varied, depending on the recombinant antigens tested (14), and changes in antibody levels following suc-cessful anti-TB treatment also varied with different antigens (15)(16)(17). Inconsistent M. tuberculosis antigen-specific antibody titers were also found in populations with various levels of M. tuberculosis exposure (18).…”

mentioning

confidence: 99%

Evaluation of Antigen-Specific Immunoglobulin G Responses in Pulmonary Tuberculosis Patients and Contacts

et al. 2015

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dThis study aimed to evaluate the serodiagnostic potential of immunoglobulin G (IgG) responses to Mycobacterium tuberculosis antigens in pulmonary tuberculosis (TB) patients, recent TB contacts with latent TB infection (LTBI), and healthy subjects. Infections were assessed using tuberculin skin tests, QuantiFERON-TB Gold In-Tube tests, drug susceptibility testing, and molecular genotyping of clinical isolates. Serum IgG responses to selective M. tuberculosis antigens, including the 38-kDa and 16-kDa antigens, lipoarabinomannan (LAM), and recombinant early secreted antigen target 6 kDa (ESAT-6) and culture filtrate protein 10 kDa (CFP-10), were determined. We found that the serum IgG responses to all antigens might differentiate between active TB and LTBI, with LAM having the highest diagnostic value (area under the curve [AUC] of 0.7756, P < 0.001). Recurrent TB cases showed significantly higher IgG responses to 38 kDa, CFP-10 (P < 0.01), and LAM (P < 0.05) than new cases, and male patients had higher levels of antigen-specific IgG than females (P < 0.05). Conversely, drug resistance and patient body mass index did not affect IgG responses (P > 0.05). LAM-specific IgG responses differentiated between acid-fast bacillus (AFB) smearpositive and -negative patients (P < 0.01), whereas antigen-specific IgG responses did not vary with the M. tuberculosis genotype (P > 0.05). Significantly higher IgG responses to 38 kDa and 16 kDa were observed in AFB smear-negative patients than in controls. These results suggest that assessment of serum IgG responses to selective purified M. tuberculosis antigens may help improve the diagnosis of active TB, particularly for sputum smear-negative patients or recurrent cases, and these may also help to differentiate between active TB and LTBI.

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IgG, IgM and IgA antibodies against the novel polyprotein in active tuberculosis

Cited by 23 publications

References 36 publications

Comparative analysis of antibodies and lymphocytes in pulmonary tuberculosis patients with negative and positive cultures

Comparative analysis of antibodies and lymphocytes in pulmonary tuberculosis patients with negative and positive cultures

Identification of Novel RD1 Antigens and Their Combinations for Diagnosis of Sputum Smear−/Culture+ TB Patients

Evaluation of Antigen-Specific Immunoglobulin G Responses in Pulmonary Tuberculosis Patients and Contacts

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