IgE cross-reactivity of phospholipase A2 and hyaluronidase of Apis dorsata (Giant Asian Honeybee) and Apis mellifera (Western Honeybee) venom: Possible use of A. mellifera venom for diagnosis of patients allergic to A. dorsata venom

Gunasekara, Peshala; Handunnetti, Shiroma; Premawansa, Sunil; Witharana, E.W.R.A.; Dasanayake, W.M.D.K.; Ratnayake, Indra P.; Seneviratne, Suranjith L.; Dias, R.K.S.; Premakumara, G. A. S.; Silva, Rajiva de

doi:10.1016/j.toxicon.2017.07.015

Cited by 8 publications

(19 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Currently, most people pay more attention to the IgG and IgG antibodies induced by wasp venom. For example, it was demonstrated that serum IgE and IgG concentrations increased significantly during wasp sting-induced immune responses after 3 h and that IgE was associated with allergies, perhaps being the main factor causing allergic reactions after wasp stings [ 30 , 33–35 ]. In the present study, to screen new epitopes, we focussed on 4 days after the wasp sting and detected the IgM indicators.…”

Section: Discussionmentioning

confidence: 99%

Biopanning of allergens from wasp sting patients

et al. 2018

View full text Add to dashboard Cite

Objective: Wasp venom is a potentially important natural drug, but it can cause hypersensitivity reactions. The purpose of the present study was to systematically study the epitopes of wasp venom. Methods: Using a random 12-peptide phage library, we performed antibody-binding epitope panning on ten serum samples from wasp sting victims at 3 h and 4 days after the sting. The panning epitopes were identified by high-throughput sequencing and matched with wasp venom proteins by BLAST. The panned antibody-binding epitopes were verified by ELISA. Results: A total of 35 specific potential wasp venom epitopes in 4 days were identified. Amongst them, twelve peptide epitopes were matched with nine wasp venom proteins, namely, vitellogenin precursor, hexamerin 70b precursor, venom carboxylesterase-6 precursor, MRJP5, major royal jelly protein 8 precursor, venom acid phosphatase Acph-1 precursor, phospholipase A2, venom serine protease 34 precursor, and major royal jelly protein 9 precursor. The changes in serum IgM antibodies induced by wasp venom were confirmed by ELISA based on the 12 peptide epitopes. Conclusion: The nine wasp venom proteins are potential allergens, which should be excluded or modified in the potential biomedical applications of wasp venom.

show abstract

Section: Discussionmentioning

confidence: 99%

Biopanning of allergens from wasp sting patients

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Briefly, the presence of orange or red metasomal segments I and II followed by posterior black segments and ventrally well-defined, nearly contiguous metapleural punctures. The venom of V. affinis was extracted by electrical stimulation as described previously [30]. Briefly, V. affinis were stimulated using a voltage which could eject venom from the stinger on to a glass plate without killing the hornets.…”

Section: Methodsmentioning

confidence: 99%

“…SDS-PAGE was performed according to the method described previously [30]. Briefly, 10 µl of venom together with 5 µl of 3× Lammelli sample buffer was incubated at 95 °C for 5 min.…”

Section: Methodsmentioning

confidence: 99%

“…Immunoblotting was carried out to determine the specific IgE to venom components [30]. Briefly, the venom proteins were first separated by SDS-PAGE and were subsequently transferred to a nitrocellulose membrane using a mini protein tetra system (Bio-Rad).…”

Section: Methodsmentioning

confidence: 99%

“…However, cross-reactivity due to cross-reactive carbohydrate determinants (CCD) would need to be excluded [28, 29]. True cross reactivity has been identified between two bee species; Apis dorsata (Giant Asian Honeybee) and Apis mellifera (Western honeybee) [30]. Characterization of the venom of V. affinis is still in its infancy when compared to V. vulgaris (wasp prevalent in Western countries) Vespa crabro L. (hornet in Western countries) or Polybia paulista I.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Diagnosis of Vespa affinis venom allergy: use of immunochemical methods and a passive basophil activation test

Gunasekara

Handunnetti

Premawansa

et al. 2019

Allergy Asthma Clin Immunol

Self Cite

View full text Add to dashboard Cite

BackgroundAllergy to Vespa affinis venom is common in the Asia Pacific region. Venom preparations for diagnosis are not commercially available for this species.MethodsThe prominent allergens in V. affinis venom were identifiedusing immunochemical methods. Use of ImmunoCAP of Vespula vulgaris crude venom/its components and a passive basophil activation test (BAT) in the diagnosis of patients who had anaphylaxis to V. affinis venom (n = 30) were also accessed. The IgE double-positivity rates (positive to both hornet and honeybee) in ImmunoCAP and the passive BAT were determined.ResultsHigh IgE reactivity was seen with the five allergens in V. affinis venom; 96% (29/30) for 34 and 24 kDa, 93% (28/30) for 45 kDa and 90% (27/30) reactivity for the 100 and 80 kDa respectively. IgE cross-reactivity was low with ImmunoCAP using V. vulgaris venom (43%; 13/30) and Ves v1 (3%; 1/30), but relatively high with Ves v5 (73%; 22/30). All patients (100%) were positive to V. affinis venom in passive BAT. In ImmunoCAP, a high double-positivity rate (76%; 23/30) was detected while no double-positivity was detected in passive BAT.ConclusionsHigh IgE reactivity for five allergens of V. affinis points to the potential of using these allergens in component resolved diagnosis (CRD). The passive BAT has shown its importance as a promising diagnostic tool with high accuracy. It would be particularly useful in cases with doubtful double-positive results of other diagnostic tests.

show abstract