Antioxidant activity and irritation property of venoms from Apis species

Somwongin, Suvimol; Chantawannakul, Panuwan; Chaiyana, Wantida

doi:10.1016/j.toxicon.2018.02.049

Cited by 56 publications

(51 citation statements)

References 35 publications

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“…The IS of 1% w/v SLS was 14.0 ± 0.2. It was well in accordance with the previous study of Somwongin et al (2018), who reported that 1% w/v SLS induced severe irritation with the IS of 10.6 ± 0.8 [42]. All signs of irritation, including hemorrhage, coagulation, and vascular lysis, were detected in the CAM exposed to 1% w/v SLS after 60 min, as shown in Figure 4.…”

Section: Irritation Profiles Of C Militaris Extractssupporting

confidence: 92%

“…DPPH, ABTS, and FTC assays were associated with the electron transfer reaction and represented the free radical scavenging activity of the tested compounds [40,41]. In contrast, FRAP assay was associated with the ion reduction process that represented the reducing ability, i.e., an ability of the tested compound to transform ferric ion (Fe 3+ ) to ferrous ion (Fe 2+ ) [42].…”

Section: Antioxidant Activities Of C Militaris Extractsmentioning

confidence: 99%

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Enhancement of Chemical Stability and Dermal Delivery of Cordyceps militaris Extracts by Nanoemulsion

et al. 2020

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This study aimed to develop nanoemulsions for enhancing chemical stability and dermal delivery of Cordyceps militaris extracts. C. militaris was extracted by maceration and infusion. The extracts were investigated for cordycepin, phenolic, and flavonoid content. The antioxidant activity was investigated by in vitro spectrophotometric methods. The irritation profile was investigated by hen’s egg-chorioallantoic membrane test. Nanoemulsions were developed using high-pressure homogenizer. C. militaris extract was incorporated into the nanoemulsion and investigated for safety, release profile, permeation, and skin retention. The results demonstrated that water extract (CW) contained the significantly highest content of cordycepin, phenolics, and flavonoids, which were responsible for antioxidant activity. CW was the most potent antioxidant. CW possessed comparable 2,2′-diphenyl-1-picrylhydrazyl radical scavenging activity and lipid peroxidation inhibition to l-ascorbic acid (96.9 ± 3.1%) and alpha-tocopherol (87.2 ± 1.0%). Consequently, ten mg/mL of CW was incorporated into nanoemulsions composing of sugar squalene, Tween® 85, and deionized water. Nanoemulsion, which had the smallest internal droplet size (157.1 ± 2.6 nm), enhanced the stability of CW, had no cytotoxicity effect and no skin irritation, released the most CW (0.9 ± 0.0% w/w after 24 h), and delivered the highest CW into the skin layer (33.5 ± 0.7% w/w). Therefore, nanoemulsion was suggested for enhancing the stability and dermal delivery of CW.

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Section: Irritation Profiles Of C Militaris Extractssupporting

confidence: 92%

Section: Antioxidant Activities Of C Militaris Extractsmentioning

confidence: 99%

Enhancement of Chemical Stability and Dermal Delivery of Cordyceps militaris Extracts by Nanoemulsion

et al. 2020

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“…The irritation of 0.1% w/w O. sanctum ethanolic extract solution, blank nanodelivery systems, and nanodelivery systems containing 0.1% w/w O. sanctum ethanolic extract was investigated using hen's egg test on the chorioallantoic membrane (HET-CAM) with slight modifications [15][16][17]. This experiment was convenient and famous because the ethical approval did not need to be applied when the age of animal's embryo was less than half of the total incubation period [18].…”

Section: Irritation Study By Hen's Egg Test On the Chorioallantoic Mementioning

confidence: 99%

Dermal Delivery Enhancement of Natural Anti-Ageing Compounds from Ocimum sanctum Linn. Extract by Nanostructured Lipid Carriers

et al. 2020

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This study aimed to develop nanodelivery systems for enhancing the Ocimum sanctum Linn. extract delivery into the skin. Rosmarinic acid (RA) was used as a marker for the quantitative determination of the extract by high-performance liquid chromatography. Nanostructured lipid carriers (NLC), nanoemulsion, liposome, and niosome, were developed and characterized for internal droplet size, polydispersity index (PDI), and zeta potential using photon correlation spectroscopy. Irritation properties of each formulations were investigated by hen’s egg test on the chorioallantoic membrane. In vitro release, skin permeation, and skin retention are determined. NLC was suggested as the most suitable system since it enhances the dermal delivery of RA with the significant skin retention amount of 27.1 ± 1.8% (p < 0.05). Its internal droplet size, PDI, and zeta potential were 261.0 ± 5.3 nm, 0.216 ± 0.042, and −45.4 ± 2.4 mV, respectively. RA released from NLC with a sustained release pattern with the release amount of 1.29 ± 0.15% after 24 h. NLC induced no irritation and did not permeate through the skin. Therefore, NLC containing O. sanctum extract was an attractive dermal delivery system that was safe and enhanced dermal delivery of RA. It was suggested for further used as topical anti-ageing products.

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“…In spite of the promising properties that are attracting much interest from pharmacological and biotechnological fields, MLT (whose initial structure is helical, when embedded in a lipid bilayer) can change to a dimeric form that destabilizes and alters artificial lipid bilayers containing dioleoylphosphatidylcholine (Somwongin et al, 2018). Therefore, MLT may induce morphologic changes in a dose-dependent manner, being a nonselective cytolytic peptide that may induce the lysis of red blood cells and human peripheral blood lymphocytes, among others (Gajski et al, 2016).…”

Section: Bioactive Compounds Of Bee Venom and Their Biological Effectsmentioning

confidence: 99%

“…Indeed, while in small doses MLT has antiinflammatory effects, increases capillary permeability, in higher doses it may cause inflammation, itching, irritation and local pain (Lee and Bae, 2016). According to the literature, this compound may cause slight, moderate and severe irritation at 0.5 mg/ml, 1 mg/ml and concentrations above 2 mg/ml, respectively (Somwongin et al, 2018). The mechanism underlying toxicity is based in the disruption of phospholipid bilayers which lead to mast cells' lysis and the release of compounds like lysosomal enzymes, histamine and serotonin, triggering pain and inflammation.…”

Section: Bioactive Compounds Of Bee Venom and Their Biological Effectsmentioning

confidence: 99%

An overview of the bioactive compounds, therapeutic properties and toxic effects of apitoxin

Pascoal

Estevinho

Choupina

et al. 2019

Food and Chemical Toxicology

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Melittin (MLT), also known as allergen Api m4 (Hossen et al., 2017), is the main compound of BV, comprising approximately 40-60% of its dry weight. MLT (together with apamin) has been reported to be found only in bee venom produced by the genus Apis (Moreno and Giralt, 2015). It is a linear, amphipathic, cationic and a-helical polypeptide molecule composed of 26 amino acid residues and is soluble in water (Silva et al., 2015; Kachel et al., 2018). Recent studies provided evidence on several biological properties of MLT (Table 1). MLT has been reported to be a very potent anti-inflammatory agent, being 100 times more potent than hydro-cortisol in animal models (Vick and Shipman, 1972; Eze et al., 2016). The antiinflammatory activity of this compound has been explored in several settings, including acne vulgaris, neuro inflammation, atherosclerosis, arthritis and liver inflammation (Lee and Bae, 2016).

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Antioxidant activity and irritation property of venoms from Apis species

Cited by 56 publications

References 35 publications

Enhancement of Chemical Stability and Dermal Delivery of Cordyceps militaris Extracts by Nanoemulsion

Enhancement of Chemical Stability and Dermal Delivery of Cordyceps militaris Extracts by Nanoemulsion

Dermal Delivery Enhancement of Natural Anti-Ageing Compounds from Ocimum sanctum Linn. Extract by Nanostructured Lipid Carriers

An overview of the bioactive compounds, therapeutic properties and toxic effects of apitoxin

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