IgE-binding epitopes of enolases, a class of highly conserved fungal allergens

Simon-Nobbe, Birgit; Probst, Gerald; Kajava, Andrey V.; Oberkofler, Hannes; Susani, Markus; Crameri, Reto; Ferreira, Fátima; Ebner, Christof; Breitenbach, Michael

doi:10.1067/mai.2000.110799

Cited by 105 publications

(91 citation statements)

References 28 publications

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“…Some of the intracellular allergens and self-antigens that also occur in a similar structure within humans that have been described so far include: (Valenta et al, 2009) 15 enolases and several calcium-binding proteins, in the case of fungal allergies (Spinozzi et al, 2008;Achatz et al, 1996;Breitenbach et al, 1997;Engel et al, 1997;Unger et al, 1999;Simon-Nobbe et al, 2000) 16 malate dehydrogenase, triose phosphate isomerase and profiling in allergies to watermelon (Pastor et al, 2009) and other fruits.…”

Section: Mild and Severe Damage Resulting From The Mechanisms Of Apopmentioning

confidence: 99%

Hypothesis for a common-cause underlying both autoimmune-diseases and allergies

Arneth

2011

IJIS

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Abstract:Although autoimmune diseases and allergies have recently been associated with each other, the results of the few studies that are available are contradictory. While some of these studies suggest that there is no evidence to assume a connection between autoimmune diseases and allergies, other studies, including some recent animal studies, suggest a connection between these diseases. In this study, we describe the possible connection between autoimmune diseases and allergies. It is important to note that, for the considerations described here, it will be necessary to consider the two types of diseases in a completely different way than has been done so far.Keywords: autoimmunity; antigen presentation; food allergies; T-lymphocytes; CD4-T-lymphocytes; CD8-T-lymphocytes; allergies.Reference to this paper should be made as follows: Arneth, B.M. (2011) 'Hypothesis for a common-cause underlying both autoimmune-diseases and allergies', Int.

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Section: Mild and Severe Damage Resulting From The Mechanisms Of Apopmentioning

confidence: 99%

Hypothesis for a common-cause underlying both autoimmune-diseases and allergies

Arneth

2011

IJIS

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Section: Resultsmentioning

confidence: 99%

“…(14,15) In baker's yeast (Saccharomyces cerevisiae), enolase may comprise up to 2% of the total protein concentration (25) ; due to its important role in glycolysis, this enzyme is expressed throughout the lifecycle of the organism. (15) Enolase has been localized microscopically in the fungal cell wall and the cytosol, and is secreted during hyphal growth. Enolase has been sequenced, cloned, and identified as an immunodominant antigen for several fungal species including S. cerevisiae, (26)(27)(28) Candida albicans, (25,27,(29)(30)(31)(32) Cladosporium herbarum, (15) Curvularia lunata, (33) Alternaria alternata, (15) Penicillium citrinum,…”

Section: Resultsmentioning

confidence: 99%

“…Enolase, a 45-50 kDa enzyme that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in fungal glycolysis, is present within the cytosol and cell wall, and is secreted during hyphal growth of various fungi. (14,15) Based on these data, C. globosum enolase was selected as a candidate biomarker for the detection of C. globosum. In the present study, C. globosum enolase was cloned and a recombinant was expressed in Escherichia coli for the production of MAbs.…”

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confidence: 99%

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Production of aChaetomium globosumEnolase Monoclonal Antibody

Green

Nayak

Lemons

et al. 2014

Monoclonal Antibodies in Immunodiagnosis and Immunotherapy

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Chaetomium globosum is a hydrophilic fungal species and a contaminant of water-damaged building materials in North America. Methods to detect Chaetomium species include subjective identification of ascospores, viable culture, or molecular-based detection methods. In this study, we describe the production and initial characterization of a monoclonal antibody (MAb) for C. globosum enolase. MAb 1C7, a murine IgG 1 isotype MAb, was produced and reacted with recombinant C. globosum enolase (rCgEno) in an enzyme-linked immunosorbent assay and with a putative C. globosum enolase in a Western blot. Epitope mapping showed MAb 1C7 specific reactivity to an enolase decapeptide, LTYEELANLY, that is highly conserved within the fungal class Sordariomycetes. Cross-reactivity studies showed MAb 1C7 reactivity to C. atrobrunneum but not C. indicum. MAb 1C7 did not react with enolase from Aspergillus fumigatus, which is divergent in only two amino acids within this epitope. The results of this study suggest potential utility of MAb 1C7 in Western blot applications for the detection of Chaetomium and other Sordariomycetes species.

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“…Another example is the cross-reaction of Curvularia lunata major allergen Cur 13 with several fungi and grasses [48]. To summarize, a large number of recombinant mold allergens have similarities to one or several other fungal enzymes [49][50][51]. Fungal allergens have been recently characterized by specifying whether or not the respective cross-reactive allergen may be found within one fungal phylum, all fungal phyla, or even non-fungal species [52].…”

Section: Discussionmentioning

confidence: 99%

Cutaneous yeast microflora in patients with atopic dermatitis

Zinkevičienė¹,

Vaiciulioniene²,

Baranauskiene³

et al. 2011

Open Medicine

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AbstractThe skin of persons with atopic dermatitis (AD) is very susceptible to cutaneous infection, and some yeast species may also aggravate AD. The total yeast population of an AD patient’s skin and its relation with individual age and body part remains poorly characterized. The aim of this study was to clarify the differences in cutaneous yeast flora by age and body parts of AD patients.By swabbing affected body parts (hands, legs, face, neck or trunk), 241 samples were collected from patients with AD (132 children and 109 adults), and as controls, 40 samples were taken from healthy individuals (20 children, 20 adults).In all, 89 (36.9%) of samples were positive; the yeast isolated belonged to three genera: Candida (27.4%), Malassezia (6.6%), and Rhodotorula (2.9%). Cutaneous colonization with yeasts was two-fold higher in the adults than in children (P<0.0001). The distribution of the yeast species was dependent on the body part sampled: Malassezia predominated in the face, neck, and trunk regions (P=0.0047); Candida more frequently colonized hands and legs (P=0.0029).Our study showed that cutaneous yeast flora and distribution of yeast species depends significantly on the age of the AD patient and the body part affected by atopic dermatitis.

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IgE-binding epitopes of enolases, a class of highly conserved fungal allergens

Cited by 105 publications

References 28 publications

Hypothesis for a common-cause underlying both autoimmune-diseases and allergies

Hypothesis for a common-cause underlying both autoimmune-diseases and allergies

Production of aChaetomium globosumEnolase Monoclonal Antibody

Cutaneous yeast microflora in patients with atopic dermatitis

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